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Báo cáo khoa học: The influence of temperature and osmolyte on the catalytic cycle of cytochrome c oxidase
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The influence of temperature on cytochromec oxidase (CCO) catalytic activity was studied in the temperature range 240–308 K. Temperatures below 273 K required the inclusion of the osmolyte ethylene glycol. For steady-state activity between 278 and 308 K the activation energy was 12 kcalÆmol )1 ; the molecular activity or turnover number was 12 s )1 at 280K in the absence of ethylene glycol. CCO activity was studied between 240and 277 K in the presence of ethylene glycol.
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