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Báo cáo khoa học: The role in the substrate specificity and catalysis of residues forming the substrate aglycone-binding site of a b-glycosidase
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The relative contributions to the specificity and catalysis of aglycone, of residues E190, E194, K201 and M453 that form the aglycone-binding site of ab-glycosidase from Spodoptera frugiperda(EC 3.2.1.21), were investi-gated through site-directed mutagenesis and enzyme kinetic experiments.
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