Báo cáo khoa học: THE THEODOR BU¨CHER LECTURE Investigating signal transduction with genetically encoded fluorescent probes

Ca 2+ and cAMP are ubiquitous second messengers in euk-aryotes and control numerous physiological responses ran-ging fromfertilization to cell death induction 1 . Todistinguish between these different responses, their subtle regulation in time, space and amplitude is needed. Therefore, the char-acterization of the signalling process requires measurement of secondmessengers with tools of precise localization, high dynamic range and as little disturbance of cell physiology as possible.