Báo cáo Y học: Caged O2 Reaction of cytochrome bo3 oxidase with photochemically released dioxygen from a cobalt peroxo complex

We developed the synthesis of the caged oxygen donor (l-peroxo)(l-hydroxo)bis[bis(bipyridyl)cobalt(III)] complex (HPBC) as nitrate salt,which has,compared with the perchlorate-form described previously [MacArthur,R., Sucheta,A.,Chong,F.F. & Einarsdottir,O¨ . (1995) Proc. Natl Acad. Sci. USA, 92,8105–8109],greatly enhanced solubility. Now,the quantum efficiency of the photolytical release of dioxygenwas determined tobe 0.4 per photon at a laser wavelength of 308 nm,which was used to observe biological reactions....