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Báo cáo Y học: Differences in the binding capacity of human apolipoprotein E3 and E4 to size-fractionated lipid emulsions
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We describe sensitive new approaches for detecting and quantitatingprotein–lipid interactionsusinganalytical ultra-centrifugation and continuous size-distribution analysis [Schuck (2000)Biophys. J.78,1606–1619]. The newmethods were developed to investigate the binding of human apo-lipoprotein E (apoE) isoforms to size-fractionated lipid emulsions, and demonstrate that apoE3 binds preferentially tosmall lipidemulsions,whereas apoE4exhibits apreference for large lipid particles.
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