Báo cáo Y học: O-GalNAc incorporation into a cluster acceptor site of three consecutive threonines Distinct specificity of GalNAc-transferase isoforms
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O-Glycosylation of three consecutive Thr residues in a fluorescein-conjugated peptide PTTTPLK)which mimics a portion of mucin 2)by four isozymes of UDP-N-ace-tylgalactosaminyltransferases (pp-GalNAc-T1, T2, T3, or T4) was investigated. Partially glycosylated versions of this peptide, PT*TTPLK, PTTT*PLK, PT*TT*PLK, PTT*T*PLK, PT*TTPLK, andPTTT*PLK(*,N-acetyl-galactosamine;, galactose), were also tested.
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