Báo cáo Y học: Purification and properties of the extracellular lipase, LipA, of Acinetobacter sp. RAG-1
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An extracellular lipase, LipA, extracted fromAcinetobacter sp. RAG-1 grown on hexadecane was purified and proper-ties of the enzyme investigated. The enzyme is released into the growth medium during the transition to stationary phase. The lipase was harvested from cells grown to sta-tionary phase, and purified with 22% yield and 10-fold purification. The protein demonstrates little affinity for anionexchange resins,withcontaminatingproteins removed by passing crude supernatants over a Mono Q column....
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