Báo cáo Y học: Purification and characterization of two secreted purple acid phosphatase isozymes from phosphate-starved tomato (Lycopersicon esculentum) cell cultures
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Two secreted acid phosphatases (SAP1 and SAP2) were markedly up-regulated during Pi-starvation of tomato suspension cells.SAP1 and SAP2 were resolved during cat-ion-exchange FPLC of culture media proteins from 8-day-old Pi -starved cells, and purified to homogeneity and final p-nitrophenylphosphatehydrolyzing specificactivitiesof 246 and 940lmol Pi producedÆmin )1 mgÆprotein )1 , respect-ively.SDS/PAGE, periodic acid-Schiff staining and analyt-ical gel filtration demonstrated that SAP1 and SAP2, respectively, exist as 84 and57 kDa glycosylatedmonomers....
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