Báo cáo Y học: The membrane-bound [NiFe]-hydrogenase (Ech) from Methanosarcina barkeri : unusual properties of the iron-sulphur clusters
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The purified membrane-bound [NiFe]-hydrogenase from Methanosarcina barkeriwas studied with electron para-magnetic resonance (EPR) focusing on the properties of the iron–sulphur clusters. TheEPRspectra showed signals from threedifferent [4Fe)4S] clusters.Twoof the clusters couldbe reduced under 101 kPa of H2, whereas the third cluster was only partially reduced.
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