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Báo cáo Y học: Ubiquitination of soluble and membrane-bound tyrosine hydroxylase and degradation of the soluble form
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Tyrosine hydroxylase (TH) demonstrates by two-dimen-sional electrophoresis amicroheterogeneitybothas a soluble recombinant humanTH(hTH1) andas amembrane-bound bovine TH (bTHmem). Part of the heterogeneity is likely due to deamidation of labile asparagine residues. Wild-type (wt)-hTH1was foundtobeasubstrate for theubiquitin(Ub) conjugating enzyme systemina reconstitutedin vitrosystem.
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