Citrus tristeza virus variants and their distribution in mandarin orchards in northeastern Himalayan Hill region of India
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Northeastern Himalayan hill region of India is known to produce quality mandarin (C. reticulata) commercially. Citrus tristeza virus (CTV), an aphid (Toxoptera citricidus) transmitted closterovirus, is a major problem to cause decline of mandarin in this region. The different orchards of Mirik in the Darjeeling and Rumtek of East Sikkim were surveyed. CTV incidence of 48-53.3% in Mirik and 40-60% in Rumtek were estimated using direct antigen coated-ELISA and polymerase chain reaction.
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