Đề tài: Khuếch đại vùng gen ITS1 - 5,8 S - ITS2 các nguồn nấm Beauveria basiana có tính độc cao (part 7)

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Beauveria bassiana tạo rất nhiều bào tử, bào tử thường có hình cầu, hình trứng, có khi chiều dài lên tới 20 m với 1 m chiều rộng. Các sợi nấm khí sinh mang các giá bào tử trần ngắn, phồng to 3 - 5 x 3 - 6 m. Các giá bào tử này hoặc tạo thành các nhánh ở phần ngọn, hoặc trực tiếp tạo thành các tế bào sinh bào tử trần

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58 PHỤ LỤC Phụ lục I: Thành phần các hóa chất trong phản ứng PCR TAE buffer 50X: +Tris-HCl 242 g/l. +Glacial acetic acid 57,1 ml/l. +EDTA 0,5M, pH 8 100 ml/l. TE +Tris HCl 10 mM, pH 8,3. +EDTA 1mM. Loading buffer +100 % glycerol 5 ml. +TE 5X buffer 2,5 ml. +Bromphenol Blue 0,015 ml. Ethidium bromide +15 l Ethidiumbromide. +300 ml TAE 0,5 X. Ladder +65 % TAE 0,5X. +25 % loading dye 6X. +15 % ladder.
59 Phụ lục II: Kết quả đọc trình tự với primer ITS4
60 Phụ lục III: Kết quả đọc trình tự PUL-Q2-4 với primer ITS5
61 Phụ lục IV: Kết quả đọc trình tự RN-LA-10 với primer ITS4
62 Phụ lục V: Kết quả đọc trình tự RN-LA-10 với primer ITS5