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Nghiên cứu điều kiện thu nhận Chondroitin sulfate (CS) từ sự thủy phân sụn ức gà bằng enzyme Alcalase

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Bài viết xác định điều kiện thủy phân tối ưu sụn ức gà bằng enzyme Alcalase 2.4L để hiệu suất thi nhận Glycosaminoglycans sulphate đạt cao nhất.

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Nội dung Text: Nghiên cứu điều kiện thu nhận Chondroitin sulfate (CS) từ sự thủy phân sụn ức gà bằng enzyme Alcalase

  1. 117 (2017) 086-092 Nguy n Th Ng c Bình*, Tr nh Th Th a Ân, Nguy n Uy Th , ng Th i h c Bách k i h c Qu c gia Thành ph H Chí Minh n Tòa so n: 20-6-2016; ch p nh -02-2017 Tóm t t Glycosaminoglycans sulphate (GAGs) s . là Chondroitin sulfate (CS) . Glycosaminoglycans sulphate sulphate .K o 4%v/wpro ian = 212 phút. Glycosaminoglycans sulfate 27,7 Glycosaminoglycans sulfate Glycosaminoglycans sulfate là 53,61%; Chondroitin sulfate Abstract Glycosaminoglycans sulphate (GAGs) belonging to a family of proteoglycans are complex natural polysacchrides in animals. Chondroitin sulfate (CS) is a major class of Glycosaminoglycans sulphate used for treatment of arthritis, especial osteoarthritis. Glycosaminoglycans sulphate can be extracted from cartilage of as cows, pigs, chicken, sharks, etc. The extraction of Glycosaminoglycans sulphate from agricultural waste sources for food supplements is meaningful for Vietnam to substitute the exported material. Our research is aimed to determine the optimal condition of hydrolysis of chicken keel cartilage by Alcalase 2.4 L to obtain Glycosaminoglycans sulphate. The optimal values for the hydrolysis process are pH at 7,0; temperature at 540C; the ratio of enzyme and substances at 4 %v/wpro and hydrolysis time of 212 minutes. Meanwhile, Glycosaminoglycans sulphate sulfate content are obtained according to the predictions of the model at 27,78% (dry weight of raw material). Glycosaminoglycans sulphate powder product was found to be Glycosaminoglycans sulphate: 53,61% (dry basis); Chondroitin sulfate determined by HPLC: 37,32% (dry basis). Keywords: Chondroitin sulfate, Glycosaminoglycan, Alcalase, chicken keel cartilage, hydrosis. * *
  2. 117 (2017) 086-092 2.3.2. nh h at tính c a enzyme Alcalase 2.4L o o 2.3.3. ng GAGs sulfate Carcharhinus Sorrah Dasyatis Kuhlii 2.1. V t li u o Bacillus Licheniformis. 2.3.4. Phân tích th ng kê s li u 2.2. B trí thí nghi m 3.1. Thành ph n c a s n c gà o pro 2.3. tích 2.3.1. nh thành ph n c a s n c gà
  3. 117 (2017) 086-092 3.3. ng c a pH n ng GAGs GAGs GAGs 3.2. ng c a t l s c n ng GAGs GAGs GAGs GAGs GAG GAGs
  4. 117 (2017) 086-092 3.4. ng c a nhi n ng thu 0 0 GAGs 0 GAGs 0 0 0 3.7. T ut n quá trình th y phân s n c gà 0 GAGs 0 0 1 3.5. ng c a t n 0 2 ng th 3 pro pro 4 GAGs k 4 0 pro 0 0 pro 3.6. ng c a th i gian n ng thu 2 GAGs 2 GAGs GAGs 0 1 1 2 2 3 3 4 4 12 1 2 13 1 3 14 1 4 23 2 3 24 2 4 34 3 4 2 2 2 2 2 2 2 2 1 1 2 2 3 3 4 4 1 2 3 4 1 2 2 1 3 2 3 1 2 2 2 2 3 4
  5. 117 (2017) 086-092 1 2 3 4 1 2 1 3 2 3 2 2 2 Sum of Mean 1 2 3 Degree of F P Source Squares Squares Freedom (DF) value value 4 (SS) (MS) Có Regression 14 145.087 10.3634 22.274 0.000 1 Residual 16 7.4442 0.465262 0 Không 2 Lack of Fit 10 6.45082 0.645082 3.8963 0.055 3 Pure Error 6 0.99337 0.165562 pro Total 30 152.531 5.08437 Corrected 4 3.8. K t qu ki m ch ng giá tr d HLGAGs (%) t mô hình v i giá tr th c nghi m 1 2 3 4 1 2 3 4 0 pro GAGs
  6. 117 (2017) 086-092 0 pro
  7. 117 (2017) 086-092 [5]. D. Uebelhart et al (2004) Intermittent treatment of knee osteoarthritis with oral chondroitin sulfate: a one-year, randomized, double-blind, multicenter study versus placebo. Osteoarthritis Cartilage, vol. 12, 269-276. [6]. M.T. Osterman et al (2007) Current and future anti- TNF therapy for inflammatory bowel disease. Curr Treat Options Gastroenterol, vol. 10, 195-207. [7]. J.W. Cho et al (2007) Curcumin attenuates the expression of IL- -6, and TNF- cyclin E in TNF- -treated HaCaT cells; NF- MAPKs as potential upstream targets. International journal of molecular medicine, vol. 19, 469-474. [8]. C. Jomphe et al (2007) Chondroitin sulfate inhibits 0 the nuclear translocation of nuclear factor-kappaB in pro interleukin-1beta stimulated chondrocytes. Basic Clin Pharmacol Toxicol, vol. 102, 59-65. [9]. kuhlii) và cá nhám ( , vol. 16, 26-30. [10]. W.R. Farndale, D.J. Buttle, A.J. Barrett (1986) Improved quantitation and discrimination of sulphated glycosaminoglycans by use of dimethylmethylene blue. Biochimca Biophysica Acta 883, 173 177. [11]. S.C. Shin et al (2006) Study on Extraction of Mucopolysaccharide-protein Containing Chondroitin Sulfate from Chicken Keel Cartilage. Asian- Australasian Journal of Animal Sciences, vol. 19, 601-604. [12]. C.Y. Cheng et al, "Optimization of microwave- Tài li u tham kh o assisted extraction of chondroitin sulfate from tilapia byproduct by response surface methodology," [1]. Johanne Martel-Pelletier, Aina Farran, Eulàlia Consumer Electronics, Communications and Montell, Josep Vergés and Jean-Pierre Pelletier Networks (CECNet), 2011 International Conference (2015) Discrepancies in Composition and Biological on, pp. 1462-1465, 2011. Effects of Different Formulations of Chondroitin Sulfate. Molecules 20, 4277-4289. [13]. C. Kun-Nan and C. Ming-Ju, "Statistical Optimization: Response Surface Methodology [2]. J.C.F. Kwok et al (2012) Chondroitin sulfate: A key Optimization in Food Engineering," in Optimization molecule in the brain matrix. The International in Food Engineering, Ferruh Erdogdu, Ed. New York, Journal of Biochemistry & Cell Biology, vol. 44, 582- USA: Taylor & Francis Group, 2008, ch. 6, pp. 115- 586. 141. [3]. Tadahisa Mikami et al (2013) Biosynthesis and [14]. AOAC, Official Methods of Analysis: The function of chondroitin sulfate. Biochimica et Association of Offiial Analytical Chemists. Biophysica Acta, vol. 1830, 4719 4733. Washington DC, USA, 1999. [4]. D. Uebelhart et al (1998) Effects of oral chondroitin sulfate on the progression of knee osteoarthritis: a pilot study. Osteoarthritis and cartilage, 39-466.
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