KY Y E U C A C D E TAI, D U A N N G H I E N C L f U KHOA H O C & C O N G N G H E<br />
<br />
<br />
DE TAI:<br />
<br />
NGHIEN CLfU SAN XUAT PHAN SINH HOC DA CHUNG<br />
<br />
BON CHO LUA CAO SAN, BAP LAI, MIA DUdNG VA KHOM<br />
<br />
TRONG TINH HAU GIANG<br />
<br />
<br />
Ca quan chu tri: Vien Cong nghe sinh hoc - Dai hoc Can Tho*<br />
CM nhiem de tdi: P G S . T s . C a o N g o c D i e p<br />
Thdi gian thuc hien: 10/2005 - 3/2007<br />
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<br />
I. DAT VAN DE:<br />
N g a y nay, v i e c su" d u n g phan d a m hoa hoc qua n h i e u se l a m gia t a n g ltfOng nitrat trong ntfdc<br />
ngam, hOn ntfa gia phan hda hoc l a i k h o n g n g t f n g t a n g l e n nhtfng h i e u qua stf d u n g l a i thap. M o t<br />
trong n h t f n g p h t f d n g phap g i a i q u y e t v a n de t r e n la l a m sao de' c a i t h i e n dat t r o n g cd n h i e u n i t d hdn<br />
va phtfdng each n a y cd t i n h kha t h i hdn b a n g each stf d u n g cac v i k h u a n v u n g re k i c h t h i c h stf tang<br />
trtfdng. O n g h i e n c t f u nay stf d u n g n h f f n g d d n g v i k h u a n cd ich la Gluconacetobacter diazotrophicus<br />
va Pseudomonas spp de' san xuat phan sinh hoc da c h u n g cho t f f n g l o a i cay t r d n g nhff bap, lua cao<br />
san, k h o m , m i a .<br />
II. M U C TIEU, N O I D U N G V A P H U d N G PHAP N G H I E N CU"U:<br />
1. M u c t i e u c u a d e t d i :<br />
- Stf d u n g n h f f n g d d n g v i k h u a n cd i c h la Gluconacetobacter diazotrophicus va Pseudomonas spp<br />
de san x u a t p h a n sinh hoc da c h u n g cho t f f n g l o a i cay t r o n g .<br />
- D a n h gia h i e u qua phan sinh hoc da n a n g d i e u k i e n ngoai d d n g de' t i n h h i e u qua k i n h te' so<br />
sanh v d i p h a n hda hoc.<br />
2. N d i d u n g n g h i e n c t f u :<br />
- T h i n g h i e m p h a n sinh hoc t r e n bap l a i ( G i o n g G-49)<br />
- T h i n g h i e m p h a n sinh hoc t r e n lua cao san ( G i o n g O M 3536)<br />
- T h i n g h i e m p h a n sinh hoc t r e n k h o m ( k h o m Q u e e n )<br />
- T h i n g h i e m p h a n sinh hoc t r e n m i a d f f d n g ( Gid'ng M i a D L M 24, R 570, V D 86-368)<br />
3. P h U d n g p h d p n g h i e n c t f u :<br />
T r f f d c k h i t h i n g h i e m , da't se thu theo h i n h c h e o (5 d i e m ) , d d d sau t f f 0 d e n 15 c m ( l d p da't m a t ) ,<br />
trdn d e u va l a y 1 phan ( k h o a n 0,5 k g ) p h d i k h d trong mat, dap nhd qua ray 2 m m de do p H , N t d n g<br />
sd', P de t i e u , K trao d d i , Chat h f f u c d ; Sau k h i t h i n g h i e m , dat c u n g thu nhff da t r i n h bay d phan t r e n<br />
trong tat ca cac n g h i e m thtfc [20 l d ] , n h f f n g c h i p h a n tich N tdng sd va P de t i e u .<br />
- T h i n g h i e m phan sinh hoc t r e n bap lai (Gid'ng G-49) : t h i n g h i e m cd 5 n g h i e m thffc, 4 lan lap l a i<br />
v d i 20 l d , d i e n tich 25 m / l d , d i e n tich can stf d u n g 750 m " bao g o m ca m f f d n g d a n va thoat n f f d c ; t h i<br />
2<br />
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<br />
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n g h i e m t h f f c h i e n trong 1 v u t a i 2 dia d i e m trong m o t h u y e n de d a n h gia c h i n h xac. G o m cac<br />
nghiem thffc:<br />
+ N T 1 : D d i chung<br />
+ N T 2 : B d n 180 k g N - 90 k g P 0 : 5 /ha<br />
+ N T 3 : B d n 100 kg phan sinh hoc/ha<br />
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+ N T 4 : B o n 100 k g p h a n sinh hoc/ha + 60 k g N / h a<br />
+ N T 5 : B o n 100 k g p h a n sinh hoc/ha + 60 k g N / h a + 100 l i t d i c h l e n m e n / h a @<br />
@ d i c h l e n m e n v i sinh vat tong hop I A A t u o i 3 l a n / v u cho bap<br />
- T h i n g h i e m phan sinh hoc t r e n l u a cao san ( G i o n g O M 3536) : t h i n g h i e m co 5 n g h i e m thu'c, 4<br />
lan l a p l a i v d i 20 l d , d i e n t i c h 20 m / l d , d i e n t i c h c a n su" d u n g 650 m<br />
2 2<br />
bao g o m ca mUOng dan va<br />
thoat ntfdc; t h i n g h i e m thtfc h i e n trong 2 v u l i e n tie'p de' d a n h gia c h i n h xac. G o m cac n g h i e m thu'c:<br />
+ N T 1 : B d i chung<br />
+ N T 2 : B d n 100 k g N - 60 k g P 0 2 5 /ha<br />
+ N T 3 : B d n 100 k g phan sinh hoc/ha<br />
+ N T 4 : B d n 100 k g phan sinh hoc/ha + 20 k g N / h a<br />
+ N T 5 : B d n 100 k g phan sinh hoc/ha + 20 k g N / h a + 100 l i t d i c h l e n m e n / h a @<br />
@ d i c h l e n m e n v i sinh vat tdng hop I A A t u d i 3 l a n / v u cho l u a .<br />
- T h i n g h i e m phan sinh hoc t r e n k h o m ( n h o m Q u e e n ) : t h i n g h i e m c d 5 n g h i e m thtfc, 4 l a n lap lai<br />
v d i 20 l d , d i e n t i c h 50 m71d, d i e n t i c h c a n stf d u n g 1.500 m " bao g o m ca mtfOng d a n va thoat ntfdc;<br />
t h i n g h i e m thtfc h i e n trong 1 v u trong sud't 10 hay 11 thang de d a n h g i a c h i n h xac. G o m cac nghiem<br />
9<br />
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thtfc:<br />
+ N T 1 : B d i chung<br />
+ N T 2 : B d n 4g ure, 2 g supe l a n , 4 g KC1 cho m d i gdc k h o m<br />
+ N T 3 : B d n 50 g phan sinh hoc/gdc k h o m<br />
+ N T 4 : B d n 50 g phan sinh hoc + 2 g ure, 1 g supe l a n , 2 g K C l / g d c k h o m<br />
+ N T 5 : B d n 5 0 k g phan sinh hoc + 2g ure, l g supe l a n , 2g K C l / g d c k h o m + 0,2 l i t d i c h l e n men/Ian<br />
tudi @<br />
@ d i c h l e n m e n v i sinh vat tdng hop I A A t u d i 3 lan/vu cho k h o m<br />
- T h i n g h i e m phan sinh hoc t r e n m i a d t f d n g (Gid'ng M i a B L M 24, R 5 7 0 , V B 86-368 do Cong ty<br />
M i a d t f d n g C a n T h d k h u y e n cao va cung cap): t h i n g h i e m cd 5 n g h i e m t h t f c , 4 l a n l a p l a i v d i 20 16,<br />
d i e n t i c h 50 m71d, d i e n tich can stf d u n g 1.500 m bao g o m ca m t f d n g d a n va thoat ntfdc; t h i nghiem<br />
2<br />
<br />
<br />
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<br />
thtfc h i e n trong 1 v u trong sud't 10 hay 11 t h a n g de' d a n h gia c h i n h xac. G o m cac n g h i e m thtfc:<br />
+ N T 1 : B d i chtfng<br />
+ N T 2 : B d n 200 kg N - 90 k g P Os /ha<br />
2<br />
<br />
<br />
<br />
+ N T 3 : B d n 100 k g phan sinh hoc/ha<br />
+ N T 4 : B d n 100 k g phan sinh hoc/ha + 50 k g N / h a<br />
+ N T 5 : B d n 100 k g phan sinh hoc/ha + 50 k g N / h a + 100 l i t d i c h l e n m e n / h a @<br />
@ d j c h l e n m e n v i sinh vat tdng hop I A A t u d i 3 lan/vu cho l u a .<br />
- So l i e u t h i n g h i e m dtfdc phan tich thd'ng ke theo chtfdng t r i n h E X C E L 2003, cac t r i trung binh<br />
se dtfdc t r i n h bay t r e n cac b a n g hay do t h i ( E x c e l ) v d i chu t h i c h p h a n k h a c b i e t nhd nhat cd y nghia<br />
nhtf L S B 5 9 r hay k i e m d i n h B u n c a n .<br />
III. KET Q U A N G H I E N C L / U :<br />
1. B a p l a i :<br />
B e tai t i e n hanh n g h i e n c t f u d 4 d i e m : thi tran M o t N g a n va xa N h d n N g h i a A ( h u y e n Chau<br />
T h a n h A ) : xa H d a M y va xa Phung H i e p ( h u y e n Phung H i e p )<br />
Qua n g h i e n c t f u cho thay. h i e u qua b d n 100 k g phan sinh hoc b d sung 60 k g N/ha va 100 l i t dich<br />
vi k h u a n cho nang suat k h o n g khac biet y nghia thd'ng ke d d i v d i bap bdn phan hda hoc ( 1 8 0 kg N -<br />
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90 kg P 07ha), neu so vdi luc ban dau chua trong thi ham ltfOng N tdng sd va P de tieu cao hOn luc<br />
2<br />
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<br />
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ban d a u m a c du gitfa cac n g h i e m thtfc k h o n g co stf khac b i e t y nghia. B i e u nay cho t h a y , b o n p h a n<br />
sinh hoc k h o n g l a m suy t h o a i h a m ltfOng cac chat trong dat ma v a n d a m bao nang suat hat bap.<br />
B a n g 1. Hieu qua phan sinh hoc vd phan hod hoc tren cdy bdp lai (G.49) trong tren ddt phu sa<br />
tinh Hau Giang nam 2006 (trung binh cua 4 thi nghiem d 4 dia diem trong tinh)<br />
<br />
Trong<br />
C h i e u cao Chieu dai Chieu sd'hot<br />
Nghiem ltfdng T I . 100 h o t<br />
cay trai hoanh trai /trai<br />
thtfc trai (g)<br />
(cm) (cm) (cm)<br />
(g)<br />
NT 1 164,76 11,12 11,64 56,41 220,63 16,91<br />
NT 2 224,60 18,11 13,98 126,53 474,94 23,35<br />
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NT 3 184,23 13,68 12,76 82,69 290,81 20,52<br />
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NT 4 208,94 15,88 13,67 107,71 405,50 23,06<br />
NT 5 216,11 17,43 13,80 117,15 436,00 23,16<br />
<br />
F tmh ** ** ** ** **<br />
<br />
LSD.05 15,88 2,95 0,79 19,51 86,27 3,41<br />
<br />
C.V 5,16% 12,54% 3,9% 12,91% 15,31% 10,35%<br />
<br />
2. L u a c a o san:<br />
B i e m t h i n g h i e m : xa T a n T h u a n h u y e n C h a u T h a n h A , t h i tran N a n g M a u , h u y e n V i T h i i y .<br />
T o n g k e t 4 t h i n g h i e m tai 2 dia d i e m t r e n cho thay t h a n h phan n a n g suat lua d n g h i e m thtfc co<br />
bon p h a n hoa hoc cao nhat, trong do stf khac b i e t d a n g ke la sd' b o n g l u a / b u i . K e d e n la n g h i e m<br />
thtfc b o n p h a n sinh hoc b d sung 20 k g N / h a va 100 l i t d i c h v i k h u a n . T u y n h i e n , tdng l t f d n g p r o t e i n<br />
trong gao ciia 2 n g h i e m thtfc k h o n g k h a c b i e t y nghia. B i e u nay cho thay p h a n sinh hoc v d i n h f f n g<br />
dong v i k h u a n huy d o n g d f f d n g chat c u n g cap cho cay lua n h f f n g k h o n g l a m anh h f f d n g d e n dinh<br />
dffdng ciia dat.<br />
B a n g 2. Hieu qua phan sinh hoc va phan hoa hoc tren cay lua cao sdn (OM 3536) trong tren ddt<br />
phii sa trong tinh Hau Giang [trung binh cua 4 thi nghiem]<br />
<br />
C h i e u cao Chieu dai sd'hot so' b o n g TL.1000 H a m ltfdng protein<br />
Nghiem<br />
cay lua bong lua chac lua hot lua t r o n g gao<br />
thtfc<br />
(cm) (cm) /bong lua /bui (g) (%)<br />
<br />
NT 1 74,65 17,19 43,75 2,30 25,64 6,171<br />
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NT 2 90,08 21,77 72.74 3,95 26,35 6,671<br />
<br />
NT 3 74,38 17,43 43,73 2,55 25,63 6,521<br />
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NT 4 82.38 19.75 57,67 2.94 26,08 6,431<br />
<br />
NT 5 86,23 21.27 71,08 3,18 26,34 6,440<br />
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F tinh ** n.s<br />
<br />
LSD.05 5,93 2,13 12,17 0,42 0,39 -<br />
<br />
C.V 4,72 7,08 13,88 9,23 0,96 4,82<br />
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3. K h o m : ^ ^ ^ ^<br />
D i a d i e m t h i n g h i e m xa H o a T i e n (nha o n g D a n g , nha o n g V a n ) | |<br />
xa H o a L t f u (nha o n g Sen, nha o n g V o ) .<br />
T o n g ket trung hinh 4 thi n g h i e m k h o m t a i t h i xa V i T h a n h cho H S ^ - '<br />
thay b o n phan hoa hoc va p h a n sinh hoc l a m t a n g c h i e u d a i t r a i<br />
dirdng k i n h t r a i va t r o n g l t f d n g t r a i k h o m so v d i d d i c h d n g k h o n g ^ V-/<br />
bdn phan. T u y n h i e n , p h a n b d n sinh hoc t a n g d d B r i x c i i a t r a i k h o m ^V'J^L<br />
cd y nghia t h o n g k e so v d i k h o m c h i b d n p h a n hda hoc hay k h o n g H m h 1 5 T o d n ><br />
cc mh C l i a g<br />
m t fh ><br />
b d n phan ( d d i chd'ng). D i e u n a y cho thay, p h a n sinh hoc c a i t h i e n nghiem Khom d xa Hod Luu, thi<br />
chat l t f d n g t r a i k h o m , b d n p h a n sinh hoc b d sung t h e m p h a n ntfa - ' -'<br />
V£ v T h a n h<br />
<br />
<br />
<br />
<br />
ltfOng phan hda hoc va 100 l i t v i k h u a n cho n a n g suat k h o m t r a i<br />
cao nhat so v d i dd'i c h t f n g n h t f n g k h o n g a n h h t f d n g d e n ltfOng N va P t r o n g da't.<br />
B a n g 3. Hieu quid ciia phan sinh hoc va phan hda hoc tren thdnh phan ndng suat vd do Brix ciia trdi Khom<br />
trong tren dot phen trong thixa Vi Thanh (trung hinh 4 TN)<br />
<br />
Dtfdng Trpng<br />
C h i e u cao<br />
Nghiem kinh trai ltfOng t r a i Do<br />
trai khom<br />
khom khom Brix<br />
thtfc<br />
(cm)<br />
(cm) (kg)<br />
<br />
Dd'i c h t f n g 18,30 10,32 1,083 11,470<br />
<br />
4 g ure, 2 g l a n , 4 g kali/gd'c 18,96 10,81 1,165 11,427<br />
<br />
50 g phan sinh hoc/gd'c 19,09 10,70 1,182 11,895<br />
<br />
50 g phan sinh hoc + 2 g ure, 1 g l a n , 2<br />
19,24 10,64 1,190 11,981<br />
g kali/gd'c<br />
<br />
50 g phan sinh hoc + 2 g ure, 1 g l a n , 2<br />
19,39 10,69 1,234 12,293<br />
g k a l i + 0,2 lit/gd'c<br />
<br />
LSD.05 0,30 0,32 0,038 0,423<br />
<br />
CV(%) 1,05 2,01 2,19 2,42<br />
<br />
4. M i a d t f d n g :<br />
D i a d i e m t h i n g h i e m : xa H i e p H t f n g , t h i t r a n C a y DtfOng, h u y e n P h u n g H i e p ; xa H d a T i e n , TX<br />
V i T h a n h ; xa V i n h V i e n , h u y e n L o n g M y .<br />
K e t qua t d n g hop ttf 4 t h i n g h i e m danh gia h i e u qua phan sinh hoc d 4 d i e m t r d n g m i a trong tinh<br />
H a u G i a n g cho thay b d n 100 k g p h a n sinh hoc b d sung 50 k g N / h a ( c h i b a n g A ltfOng phan dam X<br />
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k h u y e n cao) va cd t t f d i t h e m d i c h v i k h u a n l e n m e n hay k h o n g d e u cho n a n g suat va tdng ltfdng<br />
d t f d n g k h o n g thua n g h i e m thtfc m i a c h i b d n p h a n d a m va l a n h d a hoc. D i e u n a y cho thay, trong dat<br />
phii sa d t i n h H a u G i a n g . ltfOng p h a n d a m t i e t k i e m hay c h i stf d u n g b a n d a u i t . N h t f v a y , bdn phan<br />
sinh hoc t i e t k i e m dtfdc 150 k g N va 90 k g P 0 / h a , gia t h a n h c a y m i a c d the ha x u o n g n h i e u lan va<br />
2 5<br />
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n d n g d a n cd the thu nhap cao hOn. D i e u quan t r o n g la stf t a n d u n g chat t h a i m i i n m i a ciia nha may<br />
d t f d n g l a m chat m a n g cho v i k h u a n sd'ng va phat t r i e n trong d o . Stf h o a n tra p h e ' p h a m c i i a cay mia<br />
sau k h i c h e ' b i e n la b i e n p h a p k i n h t e ' k h o n g n h f f n g g i i i p cho nha m a y g i a i q u y e t chat t h a i de khong<br />
bi d n h i e m m d i t r f f d n g d d n g t h d i t a n d u n g l a m p h a n b d n sinh hoc cho c h i n h c a y m i a d f f d n g .<br />
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B a n g 4. Hieu qua cua phan sinh hoc va phan hda hoc tren thanh phan ndng suat va do Brix cua cay mia<br />
dudng trong tren ddi phu sa trong tinh Hau Giang (TB 4 thi nghiem)<br />
Chieu Dtfdng Trong<br />
So c a y<br />
cao c a y k i n h cay lu'o'ng 1 Do<br />
N g h i e m thu'c mia/ ,<br />
mia mia cay mia Brix<br />
40 m 2<br />
<br />
<br />
(m) (cm) (kg)<br />
D o i c h f f n g ( k h o n g b o n p h a n N va P) 3,10 257,88 2,47 1,42 18;66<br />
200 k g N + 90 k g P 0 / h a 2 5 3,28 308,39 2,58 1,77 19,99<br />
100 k g p h a n sinh hoc/ha 3,03 271,00 2,55 1,56 19,15<br />
100 k g p h a n sinh hoc/ha + 50 k g N / h a 3,24 296,60 2,56 1,71 19,43<br />
100 k g phan sinh hoc/ha + 50 k g N / h a +<br />
3,22 305,21 2,57 1,77 20,10<br />
100 l i t d i c h v i k h u a n l e n m e n<br />
LSD.05 n.s 17,37 n.s 0,13 n.s<br />
F tmh - ** - ** -<br />
<br />
CV(%) 4,21 5,76 3,97 5,49 4,61<br />
<br />
5. L d i n h u d n tCf stf t i e t k i e m p h d n b d n :<br />
a. Bap lai: V d i k e t qua 4 t h i n g h i e m trong t i n h H a u G i a n g , n a n g suat bap l a i ( g i o n g G 4 9 ) cd b d n<br />
180 k g N / h a va 60 k g P2O5 ttfOng dtfOng v d i n a n g suat l d b d n 100kg phan sinh hoc + 60 k g N nhtf<br />
vay t i e t k i e m dtfOc 120 k g N va 60 k g P2O5 ttfOng dtfOng v d i 260,87 k g ure va 4 0 0 k g p h a n l a n supe<br />
tdng c o n g la 1.653.915d<br />
b. Lua cao sdn: K e t qua t h i n g h i e m lua cao san d h u y e n Chau T h a n h A va h u y e n V i T h i i y cho thay<br />
nghiem thtfc b d n 100 k g N/ha - 60 k g P 05/ha cd nang suat ttfOng dtfOng v d i nghiem thtfc bdn 100 k g<br />
2<br />
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<br />
<br />
phan sinh hoc + 20 k g N/ha nen ndng dan tiet k i e m dtfOc 80 k g N/ha - 60 k g P 0 / h a . T o n g chi p h i la 2 5<br />
<br />
<br />
1.557.000d trtf d i 220.000d t i e n phan sinh hoc va dich v i khuan l e n m e n tiet k i e m dffdc 1.457.000d<br />
c. Mia dudng: T o n g k e t 4 t h i n g h i e m m i a d t f d n g trong t i n h H a u G i a n g cho thay n g h i e m thtfc b d n<br />
200 k g N / h a - 90 k g P Os cho m i a d t f d n g cd nang suat va t d n g ltfOng d t f d n g ttfOng dtfOng v d i n g h i e m<br />
2<br />
<br />
<br />
thtfc b d n 100 k g p h a n sinh hoc + 50 k g N / h a n e n n d n g d a n cd the t i e t k i e m d f f d c 150 k g N - 90 k g<br />
P 0 . N h t f v a y n d n g d a n t i e t k i e m d f f d c sd' t i e n la 2.660.000d trtf d i 200.000d t i e n p h a n sinh hoc va<br />
2 5<br />
<br />
<br />
dich v i k h u a n l e n m e n , n d n g d a n t i e t k i e m d f f d c 2.460.000d<br />
d. Khom: T m n g b i n h 4 t h i n g h i e m d 2 xa H d a L t f u va H d a T i e n cho thay n g h i e m thtfc b d n 50 g<br />
phan sinh hoc/gd'c ttfOng dtfOng v d i n g h i e m thtfc b d n 4 g ure + 1 g l a n + 4 g kali/gd'c n e n n d n g d a n<br />
tiet k i e m d f f d c 1.120.000d<br />
IV. KET L U A N V A K I E N N G H h<br />
1. K e t l u d n :<br />
Qua n h f f n g k e t qua t h i n g h i e m t r i n h b a y d phan t r e n , c h u n g t d i r i i t ra n h f f n g k e t l u a n sau:<br />
- B d n 100 k g phan sinh hoc va 60 k g N / h a (cd the b d sung d i c h v i k h u a n l e n m e n hay k h o n g ) cho<br />
nang suat bap hot tffOng d f f d n g bap b d n 180 k g N va 90 k g P 0 / h a . 2 5<br />
<br />
<br />
- B d n 100 k g phan sinh hoc + 20 k g N va 100 l i t v i k h u a n l e n men/ha cho cay lua cao san cd nang<br />
suat thap hOn lua bdn 100 k g N - 60 k g P 03/ha n h f f n g tdng l f f d n g protein trong gao/ha tffOng d f f d n g .<br />
2<br />
<br />
<br />
<br />
- B d n 100 k g phan sinh hoc va 50 k g N / h a (cd the b d sung d i c h v i k h u a n l e n m e n hay k h o n g ) cho<br />
nang suat m i a cay va t d n g l f f d n g d f f d n g / h a t f f d n g d f f d n g v d i m i a b d n 2 0 0 k g N - 90 k g P 0 / h a .2 5<br />
<br />
<br />
<br />
- B d n 50 g phan sinh hoc/gd'c cho nang suat k h o m tffdng dffdng v d i bdn 4 g ure, 1 g lan va 4 g kali/gd'c.<br />
2. K i e n n g h j :<br />
Cac cd quan chtfc nang tao d i e u k i e n tie'p tuc nghien ctfu sau hOn ve hieu qua phan sinh hoc tren lua<br />
cao san va k h o m d trong tinh H a u Giang nham t i m ra b i e n phap canh tac hieu qua va t d i ffu nhat."<br />
<br />
<br />
87<br />