Two-step pretreatment for improving enzymatic hydrolysis of spent coffee grounds

Spent coffee ground (SCG) has attracted increasing attention since it contains many useful components such as polysaccharides, protein, lipid, and bioactive compounds. The aim of this research was to enhance the enzymatic hydrolysis to release important sugars in the SCG using different pretreatment methods. Spent coffee grounds were pretreated by alkali pretreatment, organosolv pretreatment, and the combined process