Adenylation domain

Phomafungin is a recently reported broad spectrum antifungal compound but its biosynthetic pathway is unknown. We combed publicly available Phoma genomes but failed to find any putative biosynthetic gene cluster that could account for its biosynthesis.

18p visilicon2711 20-08-2021 12 1   Download

Mammalian adenylate cyclases are predicted to possess complex topologies, comprising two cassettes of six trans-membrane-spanningmotifs followedbyacytosolic, catalytic ATP-binding domain. Recent studies have begun to provide insights on the tertiary assembly of these proteins; crystal-lographic analysis has revealed that the two cytosolic domains dimerize to forma catalytic core, whilemore recent biochemical and cell biological analysis shows that the two transmembrane cassettes also associate to facilitate the functional assembly and trackingof the enzyme. ...

9p research12 23-04-2013 35 1   Download

This work presents a computational analysis of the molecular characteris-tics shared by the adenylation domains from traditional nonribosomal pep-tide synthetases (NRPSs) and the group of the freestanding homologous enzymes: a-aminoadipate semialdehyde dehydrogenase, a-aminoadipate reductase and the protein Ebony. The results of systematic sequence com-parisons allow us to conclude that a specificity-conferring code, similar to that described for the NRPSs, can be recognized in such enzymes.

13p awards 05-04-2013 32 2   Download

In our continued studies on corticotropin releasing factor receptor (CRFR1) signaling in the skin, we tested functional activity of CRFR1a, e, f, g and h isoforms after transfection to COS cells.Both membrane-bound and soluble variants are translatedin vivointofinal proteinproducts that undergo further post-translational modifications.

10p dell39 03-04-2013 38 2   Download

The interaction of the adenylate cyclase catalytic domain (AC) of the Bordetella pertussismajor exotoxin with its activator calmodulin (CaM) was studied by time-resolved fluorescence spectroscopy using three fluorescent groups located indifferent regions ofAC: tryptophan residues (W69 and W242), a nucleotide analogue (3¢-anthraniloyl-2¢-deoxyadenosine 5¢-triphosphate, Ant-dATP) and a cysteine-specific probe (acrylodan).

13p dell39 03-04-2013 22 3   Download

The human 2¢)5¢ oligoadenylate synthetases (OAS) form a conserved family of interferon-induced proteins consisting of four genes:OAS1, OAS2, OAS3and the 2¢)5¢ oligo-adenylate synthetase-like gene (OASL). When activated by double-strandedRNA,OAS1–3polymerizeATP into2¢)5¢-linkedoligoadenylates; 2¢)5¢-linkedoligoadenylates, in turn, activate a latent endoribonuclease that degrades viral and cellular RNAs. In contrast, while the p59 OASLprotein is highlyhomologous to theOAS family (45%identity), its 350 amino acid N-terminal domain lacks 2¢)5¢ oligoadenylate synthetase activity. ...

9p dell39 03-04-2013 40 3   Download

Aryl acid adenylation domains are the initial enzymes for aryl-capping of catecholic siderophores in a plethora of microorganisms. In order to over-come the problem of iron acquisition in host organisms, siderophore bio-synthesis is decisive for virulence development in numerous important human and animal pathogens.

11p dell39 27-03-2013 28 3   Download

The C-terminal catalytic domains of the 11 mammalian phosphodiesterase families (PDEs) are important drug targets. Five of the 11 PDE families contain less well-characterized N-terminal GAF domains. cGMP is the lig-and for the GAF domains in PDEs 2, 5, 6 and 11, and cAMP is the ligand for PDE10.

10p galaxyss3 21-03-2013 31 2   Download

Nonribosomal peptide synthetases serve as multidomain protein templates for producing a wealth of pharmaceutically important natural products. For the correct assembly of the desired natural product the interactions between the different catalytic centres and the reaction intermediates bound to the peptidyl carrier protein must be precisely controlled at spatial and temporal levels.

13p mobifone23 18-01-2013 40 2   Download