Antibody engineering

Ebook "Animal cell technology: Basic & applied aspects - Volume 14 (Proceedings of the 17th annual meeting of the Japanese association for animal cell technology (JAACT), Nagoya, Japan, November 15-18, 2004)" contains the proceedings of the 17th Annual and International Meeting of the Japanese Association for Animal Cell Technology (JAACT) in Nagoya, Japan, in November 2004. This volume gives the readers a complete review of the present state-of-the-art in Japan and other countries.

417p manmanthanhla0201 27-02-2024 6 2   Download

Ebook "Animal cell technology: Basic & applied aspects" ives the readers a complete review of the present state-of-the-art and will be useful for those working in either academic environments or in the biotechnology and pharmaceutical sectors, particularly cell biologists, biochemists, molecular biologists, immunologists, biochemical engineers and all other disciplines related to animal cell culture.

341p manmanthanhla0201 27-02-2024 4 1   Download

The vortex flow reactor (VFR) can be used in many chemical engineering applications. This paper assesses its novel use in the purification of monoclonal antibodies from cell broth. To this end, the IgG2a antibody was purified from the unclarified fermentation broth of transgenic mouse 55/6 hybridoma cells.

12p viginny 23-12-2022 7 3   Download

Cycle stability is important for preparative chromatography resins. Up to 200 cycles have been reported for Protein A affinity resins when used under optimized operating conditions. Through engineered ligands, alkaline resistant Protein A resins are available that can withstand repeated cleaning-in-place cycles with even 1 M NaOH.

7p vironald 15-12-2022 5 2   Download

Anti-CD3 immunotherapy was initially approved for clinical use for renal transplantation rejection prevention. Subsequently, new generations of anti-CD3 antibodies have entered clinical trials for a broader spectrum of therapeutic applications, including cancer and autoimmune diseases.

14p visilicon2711 20-08-2021 11 1   Download

Relatively poor penetration and retention in tumor tissue has been documented for large molecule drugs including therapeutic antibodies and recombinant immunoglobulin constant region (Fc)-fusion proteins due to their large size, positive charge, and strong target binding affinity.

14p vialabama2711 21-09-2020 10 0   Download

Hepatitis B viral infection is a major health problem worldwide. New cases can be prevented by immunization with hepatitis B vaccine containing the genetically engineered surface antigen. Values of ≥ 10 IU/ml are considered protective. However a small percentage of individuals fail to develop these levels. Such non responders are at risk of acquiring Hepatitis B virus infection. This is of concern especially in health care personnel. The study comprised of 100 female medical students from Mallareddy Medical College for Women, Suraram who completed the standard 3 dose course of vaccination.

7p nguaconbaynhay6 24-06-2020 25 1   Download

Antibody phage display is a proven key technology that allows the generation of human antibodies for diagnostics and therapy. From naive antibody gene libraries - in theory - antibodies against any target can be selected.

15p viuchiha2711 21-04-2020 6 0   Download

Advances in antibody engineering provide strategies to construct recombinant antibody-like molecules with modified pharmacokinetic properties. Multermerization is one strategy that has been used to produce antibody-like molecules with two or more antigen binding sites.

8p vihamax2711 21-04-2020 13 1   Download

In recognition of the enormous advances in biotechnology in recent years, we are pleased to present this Second Edition of “Biotechnology” relatively soon after the introduction of the First Edition of this multi-volume comprehensive treatise. Since this series was extremely well accepted by the scientific community, we have maintained the overall goal of creating a number of volumes, each devoted to a certain topic, which provide scientists in academia, industry, and public institutions with a well-balanced and comprehensive overview of this growing field.

658p whitedaisy1010 05-06-2013 120 33   Download

Affinity reagents capable of selective recognition of the different human immunoglobulin isotypes are important detection and purification tools in biotechnology. Here we describe the development and characterization of affinity proteins (affibodies) showing selective binding to human IgA. From protein libraries constructed by combinatorial mutagenesis of a 58-amino-acid, three-helix bundle domain derived from the IgG-binding staphylococcal protein A, variants showing IgA binding were selected by using phage display technology and IgA monoclonal antibodies (myeloma) as target molecules. ...

9p system191 01-06-2013 38 2   Download

Hybridomas are cells that have been engineered to produce a desired antibody in large amounts. To produce monoclonal antibodies, Bcells are removed from the spleen of an animal that has been challenged with the relevant antigen. These B-cells are then fused with myeloma tumor cells that can grow indefinitely in culture (myeloma is a B-cell cancer). This fusion is performed by making the cell membranes more permeable. The fused hybrid cells (called hybridomas), being cancer cells, will multiply rapidly and indefinitely and will produce large amounts of the desired antibodies.

7p zingzing09 24-04-2013 68 4   Download

Staphylokinase (SAK) is a 15.5-kDa protein fromStaphy-lococcus aureusthat activates plasminogenby forminga1 : 1 complex with plasmin. Recombinant SAK has been shown in clinical trials to induce ®brin-speci®c clot lysis in patients withacutemyocardial infarction.However, SAKelicits high titers of neutralizing antibodies. Biochemical and protein engineering studies have demonstrated the feasibility of generatingSAKvariantswith reducedantigenicity yet intact thrombolytic potency.

7p research12 23-04-2013 38 2   Download

Important factors when choosing the best breed of chickens for your situation are: price, market situation, experience, farm management, local preference and availability. The price will determine your choice. Modern hybrids are very expen- sive. They also need very good care and high quality, balanced feed to be productive. Local breeds are cheaper and better adapted to local conditions. With adequate care, they are reasonably productive. How- ever, if you want to raise chickens on a larger scale and decide to buy balanced feed, it is better to choose the more expensive hybrids. ...

19p loginnhanh 22-04-2013 65 3   Download

Economic crises are historically times of industrial renewal. Less efficient firms fail while more dynamic ones emerge and expand. Creative destruction is an essential engine of long term efficiency in market economies, and it intensifies in downturns. Available data for many OECD countries point to a sharp increase in bankruptcies and business failures in recent months. New business models and new technologies, particularly those allowing a reduction in cost, often arise in downturns, as was the case with low-cost airlines which grew out of the recession of the early 1990s.

22p trinhcaidat 19-04-2013 62 3   Download

The Communication part is the interface for programs accessing the TOE. It is the interface between the TOE and clients performing requests. All responses to user application requests return to the client through this part of the TOE. The Relational Engine is the core of the database engine and is responsible for all security relevant decisions.

0p nhacsihuytuan 13-04-2013 34 1   Download

The title of this textbook, Biosensors: From Electric Circuits to Immunosensors, implies that we are going to learn both electric circuitry (in relation to conventional sensors such as temperature sensors) and biosensors (such as antibody-based immunosensors). The idea of putting these two topics together into a single book came from my decision to add biosensor topics to the “Sensors and Controls” class taught at the University of Arizona. At that time, I realized there was no available textbook that equally addressed these two topics.

266p nguyenthai_thinh 16-03-2013 81 13   Download

Antibodies are the paradigm for binding proteins, with their hypervariable loop region supported by a structurally rigid framework, thus providing the vast repertoire of antigen-binding sites in the immune system. Lipoca-lins are another family of proteins that exhibit a binding site with high structural plasticity, which is composed of four peptide loops mounted on a stable b-barrel scaffold.

7p galaxyss3 07-03-2013 42 2   Download

In recent years, classical antibody-based affinity reagents have been challenged by novel types of binding proteins developed by combinatorial protein engineering principles. One of these classes of binding proteins of non-Ig origin are the so-called affibody binding proteins, functionally selected from libraries of a small (6 kDa), non-cysteine three-helix bundle domain used as a scaffold.

9p galaxyss3 07-03-2013 40 3   Download

Two novel human antitumor immunoconjugates, engineered by fusion of a single-chain antibody fragment against human ErbB2 receptor, termed Erbicin, with either a human RNase or the Fc region of a human IgG1 , are selectively cytotoxic for ErbB2-positive cancer cellsin vitro andin vivo.

11p cosis54 05-01-2013 48 3   Download