Antimicrobial assay

Part 2 book "Laboratory procedures for veterinary technicians" includes content: Sample collection and handling, automated analyzers, protein assays and hepatobiliary function tests, kidney function tests, pancreatic function tests,electrolytes and acid–base status, miscellaneous tests, introduction to microbiology, equipment and supplies, sample collection and handling, staining specimens, culture techniques, antimicrobial sensitivity testing, additional testing,... and other contents.

258p muasambanhan05 22-01-2024 5 2   Download

Ebook "DNA topoisomerases: Methods and protocols"cover various topics, such as type 1 topos with an evolutionary perspective; antimicrobial agents targeting topos; experimental protocols related to DNA topology, including a basic technique to measure DNA supercoiling in bacteria; protocols for in vitro studies, including assays to detect RNA topo activity; topo inhibitors; and a study of plasmid-mediated quinolone resistance in bacteria.

328p lucchinguyen 28-12-2023 5 2   Download

The growth of waterborne pathogens and the discharge of partially treated or non-treated chemical waste has become a severe environmental and health risk. Conventional wastewater treatment methods have become less effective, and most current techniques require multiple stages for the disinfection of antimicrobial-resistant (AMR) pathogens and the combined removal of toxic, non-biodegradable, or emerging priority pollutants from wastewater.

273p runordie3 06-07-2022 12 1   Download

Antimicrobial resistance (AMR) is a significant threat to human health globally. Diseases caused by AMR are getting more challenging to cure due to the increasing rate of resistance of microbial pathogens. Drugs formulated with antimicrobial peptide (AMP) can be applied to treat these diseases. AMPs are found naturally within organisms, especially amphibians, and is related to the high adaptability of defence mechanisms against various pathogens and predators in the environment.

12p dolomite36 30-12-2021 8 0   Download

The crude extracts of L. shawii and P. emblica seeds proved to be the most active antimicrobials, against the entire S. aureus strains, using both quality and quantity assays for their bactericidal activity. Both L. shawii and P. emblica seeds contained remarkable amounts from active phytochemicals, alkaloids, phenolic compounds, tannins and flavonoids.

7p schindler 30-12-2021 3 0   Download

A simple synthetic method is reported for the preparation of some new phosphorus macrocycles in which the pyrazole rings are appended to a phosphorus atom. The methodology is based on the cyclocondensation reaction of bis(4-formylpyrazolyl) phosphine oxides (1a, 1b) with nitrogen nucleophiles that contain active terminal amino groups. The antimicrobial activities of the synthesized compounds were assayed.

10p langthannam 29-12-2021 7 0   Download

Marine compound dendrodoine was first obtained from tunicate species (Dendrodo grossularia). It has a five-membered ring, namely, it is a heterocycle thiadiazole, which is found rarely in natural sources. Following its biological activities, novel analogs have been investigated recently. Synthesis of the analogs for this study is realized with uncommon thiazole closure, including methylene-carbonyl condensation. Structures are elucidated by NMR (1H, 13C) and HRMS spectrums.

9p tudichquannguyet 29-11-2021 9 1   Download

In the present work, essential oil and fatty acids and extracts obtained from aerial parts of Phlomis linearis Boiss. & Bal. were investigated for chemical composition and biological activities. The phytochemical analyses were conducted with gas chromatographymass spectrometry/flame ionisation detector (GC-MS/FID) and liquid chromatography-mass spectromtetry (LC-MS/MS) techniques. The extracts and essential oil were studied for α-amylase and acetylcholinesterase activities with two different spectrophotometric methods.

13p tudichquannguyet 29-11-2021 16 3   Download

The reaction of 3,4-dichlorophenyl-1,3,4-oxadiazole-2(3H)-thione with piperidine derivatives via Mannich reaction was used to generate eleven novel compounds in moderate to good yields. Synthesized molecules were characterized according to their structure with 1 H NMR, 13C NMR and FT-IR spectral foundations, which were compatible with literature informations. Antimicrobial activity and cytotoxicity studies were done by disc diffusion and NCI-60 sulphordamine B assay methods.

12p tudichquannguyet 29-11-2021 7 0   Download

The metabolite 2-(1,1-diallyl-but-3-enyl)-5-nonyl-phenol isolated from Streptomyces sp. TC1 exhibited antimicrobial activity against bacterial leaf blight pathogen Xanthomonas oryzae pv. oryzae (Xoo). The in vitro antimicrobial activity was found to be between 1.8-2.5 cm in agar well diffusion assay against the tested Xoo isolates and 200 µg/ml in terms of minimum inhibitory concentration. The isolated compound showed well binding with the virulence proteins of Xoo by producing a better docking score in molecular docking studies.

10p nguaconbaynhay10 22-01-2021 12 1   Download

The objective of this study was to evaluate the antioxidant, antimicrobial and chemical composition of the aqueous extract of dried Shiitake mushrooms obtained from local trade. Four methods were used for analysis of antioxidant activity: ABTS, DPPH, reducing power and chelating activity of Fe+2.

11p caygaocaolon8 07-11-2020 20 1   Download

In the present study, isolated some bacterial strains and the isolated strains were subjected to screen its antibacterial potential against human pathogenic bacteria. The antimicrobial assay showed one potential bacterium could able to produce antibacterial compound against enterobacteria members.

5p angicungduoc8 07-11-2020 13 2   Download

In the conventional broth microdilution assay, the optical density (OD) of drug dilutions, inoculated with microorganisms after sufficient incubation is taken into consideration, which correlates with the most metabolically active cells in the mid log phase of growth. The particles present in the liposomes and other particulate solutions interfere with the OD measurement and produce an erratic result. Resazurin method is more reliable and realistic as it depends on metabolic activity of cells rather than OD of particulate matter present in the samples.

8p nguathienthan8 20-10-2020 14 2   Download

The aim of the present study was to access probiotic attributes such as antimicrobial activity, acid and bile tolerance, auto aggregation capacity, hydrophobicity assay and antibiotic susceptibility of two screened potential lactic acid bacterial strains from a novel traditional fermented product (viz. Luske, prepared using fermented lassi) Solan District of Himachal Pradesh. Six isolates were isolated from the traditional fermented foods of a Trans Himalayan state of India and were subjected to biochemical characterization.

10p nguaconbaynhay8 13-10-2020 13 3   Download

As plants have substances of medicinal values, they are used to treat number of diseases since long time. They had minimal or less side effect on human beings. In the present study, the antimicrobial and antioxidant properties of the flower extract of Nerium oleander was evaluated as a remedy for pathogenic diseases. The screening of various phytochemicals was conducted using a standard procedure using different solvents. Antimicrobial activity was evaluated by the disc diffusion method.

8p cothumenhmong7 08-09-2020 21 2   Download

The present study was conducted to evaluate antimicrobial and cytotoxicactivity of Sisymbrium irio L extract against MDRB (Staphylococcus aureus, Enterococcus faecium, Klebsiella pneumoniae, Acinetobacter baumanni, Enterobacter cloacae and Pseudomonas aeruginosa) and Candida albicans. Antibiotic sensitivity profile was performed using disc diffusion method. Cytotoxicity was measured against African Green Monkey Kidney (VERO) cell line using the colorimetric MTT assay.

13p kethamoi6 29-06-2020 10 1   Download

The development of nanotechnology interests the researchers for synthesis of nanoparticles with various bio-applications. The green synthesis of copper nanoparticles using Momordica charantia fruit extract acts as both reducing and capping agent. The biosynthesized CuNps were characterized by using UV-Vis analysis, Dynamic Light Scattering (DLS), Fourier Transform Infrared analysis (FTIR), X-ray diffraction analysis (XRD), Scanning Electron Microscopy (SEM) and Transmission Electron Microscopy (TEM) analysis.

11p kethamoi6 29-06-2020 10 2   Download

The aim of the present study was to determine the distribution of pathogens and their antibiotic profile against the commonly used antibiotic therapy. A total of 5614 blood specimens from patients were received from intensive care units in Villupuram Medical College, Tamilnadu, India, were cultured by standard microbiological procedures for the isolation of pathogens.

4p chauchaungayxua6 26-06-2020 9 1   Download

The purpose of this study was to investigate the antibacterial and anti-fungal activities of four species Sargassum namely: Sargassum polycystum, Sargassum oligocystum, Sargassum crassifolium and Sargassum cristaefolium collected along the coastal areas of Diora-Zinungan Sta. Ana Cagayan, Philippines. Extracts of powdered seaweeds were prepared using sequential extraction with different organic solvents in order to increasing the polarity (Ethanol, n-hexane, dichloromethane and ethyl acetate and aqueous).

14p nguaconbaynhay6 24-06-2020 19 1   Download

Objective of the research was to validate the potential of Syzygium aromaticum and Kaempheria galanga against biofilm formation and plasmid borne drug resistance exhibited by Pseudomonas spp. Antimicrobial property of S.aromaticum and K.galanga on P.aeruginosa culture was assessed using the agar well diffusion method. Crystal violet assay was employed to determine the percentage inhibition of P.aeruginosa biofilm by the extracts following generation of biofilm on multiwell plates.

7p nguaconbaynhay6 23-06-2020 31 0   Download