Biochemical changes

Currently, along with the economic development, the urbanization process has been taking place strongly, putting great pressure on the water supply for urban areas and industrial zones, leading to conflicts, overlapping and obstacles to the water supply management.

4p vibecca 01-10-2024 2 1   Download

Identify biological characteristics, some indicators of physiological and biochemical blood and urine of civets in captivity; determine the change in sex hormones of female civets and evaluate the effect of sexual stimulation on the fertility of civets in captivity in order to preserve herd in the direction of quantity development.

27p larachdumlanat129 20-01-2021 23 3   Download

The total organic acid content in papaya was relatively low and tends to decrease from young fruit stage to ripe fruit stage. Based on this study results, Taiwanese papaya fruit should be harvested after physiological maturity and before ripening completely (from 13 weeks of age to 15 weeks of age) to ensure the nutritional value of the fruit during storage.

7p tamynhan9 02-12-2020 14 2   Download

The present study reports the seasonal and spatial changes in water quality of river Yamuna, India. Surface water samples were collected from three different stretches of river Yamuna i.e. Delhi, Mathura and Agra on seasonal basis from April 2014 to February 2015 and were analyzed for different water quality parameters i.e. water temperature, pH, electrical conductivity, total dissolved solids, total alkalinity, biochemical oxygen demand, chemical oxygen demand, dissolved oxygen, nitrates and phosphates...

19p kequaidan7 01-09-2020 29 1   Download

To investigate the changes in the haematological, serum biochemical parameters and hepato-renal biomarkers upon dietary supplementation of nano zinc oxide (nZnO) and its effect on growth performance in calves, 24 weaned (6 months age) crossbred calves were randomly distributed to four dietary plans (Control, Treatment1, Treatment2 and Treatment3). The Control(C) group was fed exclusively with basal diet; T1 was supplemented with 25ppm ZnSo4, T2 and T3 with 5ppm and 10ppm nZnO respectively, along with the basal diet. Zinc content in basal was 26.24ppm.

11p angicungduoc6 22-07-2020 15 5   Download

A field experiment was conducted at Boodamanahalli village, (Bangalore rural district) to know the effect of tikka disease, caused together by Cercospora arachidicola and Cercosporidium personatum on biochemical parameters viz., total sugars, total phenols and total proteins as influenced by different sowing dates in groundnut. It included two varieties, TMV-2 a highly susceptible one and GBPD-4 a resistant with nine sowing dates starting from December 2008 to August 2009 at monthly intervals.

9p chauchaungayxua6 26-06-2020 19 2   Download

This study was conducted to investigate the role of silibinin in the change of biochemical indices of rabbits poisoned with of amanita virosa to provide the experimental evidence on the ability of hepatocellular protection of silibinin against toxic effect of amatoxin.

9p trieusuper 13-09-2018 39 0   Download

P-glycoprotein (Pgp), a membrane pump often responsible for the multidrug resistance of cancer cells, undergoes conformational changes in the presence of substrates/modulators, or upon ATP depletion, reflected by its enhanced reactivity with the UIC2 monoclonal antibody. When the UIC2-shift was elicited by certain modulators (e.g. cyclosporin A or vinblastine, but not with verapamil or Tween 80), the subsequent binding of other monoclonal anti-Pgp Ig sharing epitopes with UIC2 (e.g. MM12.10) was abolished [Nagy, H., Goda, K., Arceci, R., Cianfriglia, M., ´ Mechetner, E. & Szabo Jr, G.

6p system191 01-06-2013 62 7   Download

Chimeric calcium/calmodulin dependent protein kinase (CCaMK) is characterized by the presence of a visinin-like Ca 2+ -binding domain unlike other known calmodulin-dependent kinases. Ca 2+ -Binding to the visinin-like domain leads to autophosphorylation and changes in the affinity for calmodulin [Sathyanarayanan P.V., Cremo C.R. & Poovaiah B.W. (2000)J. Biol. Chem. 275, 30417–30422]. Here, we report that the Ca 2+ -stimulated autophosphory-lation of CCaMK results in time-dependent loss of enzyme activity. ...

7p research12 29-04-2013 31 3   Download

Unfolding, inactivation and dissociation of the lectin from Artocarpus hirsutaseedswere studiedbychemical (guanidine hydrochloride, GdnHCl) and thermal denaturation. Con-formational transitions were monitored by intrinsic ¯uor-escence and circular dichroism. The gradual red shift in the emissionmaxima of the native protein from335 to 356 nm, change intheellipticityat 218 nmandsimultaneousdecrease in the sugar binding activity were observed with increasing concentration of GdnHCl in the pH range between 4.0 and 9.0. ...

5p research12 29-04-2013 41 3   Download

In order to study the pressure-induced changes of biological membrane, hydrostatic pressures of from 0.1 to 400 MPa were applied to membrane-bound Na + /K + -ATPase from pigkidneyas amodel systemof proteinand lipidmembrane. The activity showed at least a three-step change induced by pressures of 0.1±100 MPa, 100±220 MPa, and 220 MPa or higher.

9p research12 29-04-2013 25 2   Download

Virus infection results in the activation of a set of cellular genes involved in host antiviral defense. IRF-3 has been identifiedas a critical transcription factor in this process. The activationmechanismof IRF-3 is not fully elucidated,yet it involves a conformational change triggered by the virus-dependent phosphorylation of its C-terminus. This con-formational change leads to nuclear accumulation,DNA binding and transcriptional transactivation.

10p tumor12 22-04-2013 38 3   Download

Eur. J. Biochem. 269, 5950–5955 (2002) Ó FEBS 2002 doi:10.1046/j.1432-1033.2002.03323.x The most active factor VIIa (FVIIa) variants identified to date carry concurrent substitutions at positions 158, 296and 298 with the intention of generating a thrombin-mimicking motif, optionally combined with additional replacements within the protease domain [Persson, E., Kjalke, M. & Olsen, O. H. (2001)Proc. Natl Acad. Sci. USA98, 13583– 13588]. Here we have characterized variants of FVIIa mutatedat one or twoof these positions toassess the relative importance of the individual replacements.

6p tumor12 22-04-2013 36 2   Download

Once platelets are activated, the first event tooccur is a rapid change in shape, associated with Ca 2+ /calmodulin-dependent myosin light chain (MLC) phosphorylation and withRhokinase activation. The purpose of this studywas to investigate which is the biochemical pathway that leads to platelet shape change in response to convulxin, a selective GpVI activator, and to verify whether MLC phosphoryla-tion is essential for this process.

7p tumor12 22-04-2013 37 3   Download

Acomputational approach is used to analyse temporal gene expression in the context of metabolic regulation. It is based on the assumption that cells developed optimal adaptation strategies to changing environmental conditions. Time-dependent enzyme profiles are calculatedwhichoptimize the function of a metabolic pathway under the constraint of limited total enzymeamount.For linearmodel pathways it is shown thatwave-like enzyme profiles are optimal for a rapid substrate turnover.

8p tumor12 22-04-2013 49 3   Download

Thea-phycoerythrocyanin subunits of the different phy-coerythrocyanin complexes of the phycobilisomes from the cyanobacterium Mastigocladus laminosusperform a remarkable photochemistry. Similar to phytochromes – the photoreceptors of higher plants – the spectral pro-perties of the molecule reversibly change according to the irradiation wavelength. To enable extensive analyses,the protein has been produced at high yield by improving purification protocols.

10p tumor12 22-04-2013 45 3   Download

Mature, active mammalian histidine decarboxylase is a di-meric enzyme of carboxy-truncatedmonomers (
53 kDa). By using a biocomputational approach, we have generateda three-dimensional model of a recombinant 1/512 fragment of the rat enzyme, which shows kinetic constants similar to those of the mature enzyme purified from rodent tissues. This model, together with previous spectroscopic data, al-lowed us to postulate that the occupation of the catalytic center by the natural substrate, or by substrate-analogs, would induce remarkable changes in the conformation of the intact holoenzyme....

12p tumor12 20-04-2013 36 2   Download

It was shown previously [Riedinger, H. J., van Betteraey-Nikoleit, M & Probst, H. (2002)Eur. J. Biochem.269, 2383–2393] that initiation ofin vivo SV40 DNA replication is reversibly suppressed by hypoxia in a state where viral minichromosomes exhibit a nearly complete set of repli-cation proteins. Reoxygenation triggers fast completion and post-translational modifications.

11p tumor12 20-04-2013 30 2   Download

The currently accepted model of moult control in crusta-ceans relies entirely on the hypothesis that moult-inhibiting hormone (MIH) and crustacean hyperglycaemic hormone (CHH) repress ecdysteroid synthesis of the target tissue (Y-organ) only during intermoult, and that changes in syn-thesis and/or release of these neurohormones are central to moult control.

9p tumor12 20-04-2013 51 3   Download

Ascorbic acid is often regarded as an antioxidantin vivo, where it protects against cancer by scavenging DNA-dam-aging reactive oxygen species. However, the detailed mech-anism of the action of ascorbic acid on genetic DNA is still unclear. We examined the effect of ascorbic acid on the higher-order structure of DNA through real-time observa-tion by fluorescence microscopy. We found that ascorbic acid generates a pearling structure in single giant DNA molecules, with elongated and compact regions coexisting along a molecular chain. ...

6p tumor12 20-04-2013 32 5   Download