Biochemical

Campylobacter species are a leading cause of food-borne disease and C. jejuni highlight the most potential public health impact of Campylobacter contamination by pathogens originating from animals or animal products.. The total of 33meat samples comprising 8 from sheep (5) and goat (3) also 25 beef samples were screened by morphological, biochemical and molecular technique.

9p chauchaungayxua11 23-03-2021 17 2   Download

Identify biological characteristics, some indicators of physiological and biochemical blood and urine of civets in captivity; determine the change in sex hormones of female civets and evaluate the effect of sexual stimulation on the fertility of civets in captivity in order to preserve herd in the direction of quantity development.

27p larachdumlanat129 20-01-2021 24 3   Download

To investigate the changes in the haematological, serum biochemical parameters and hepato-renal biomarkers upon dietary supplementation of nano zinc oxide (nZnO) and its effect on growth performance in calves, 24 weaned (6 months age) crossbred calves were randomly distributed to four dietary plans (Control, Treatment1, Treatment2 and Treatment3). The Control(C) group was fed exclusively with basal diet; T1 was supplemented with 25ppm ZnSo4, T2 and T3 with 5ppm and 10ppm nZnO respectively, along with the basal diet. Zinc content in basal was 26.24ppm.

11p angicungduoc6 22-07-2020 17 6   Download

Surveying some clinical features and X-ray images of long bones, skulls, spine, blood biochemical tests and electrolytes in patients with Osteogenesis imperfecta. Evaluation of internal bone results using self-made tool to treat deformation of the lower limbs in patients with Osteogenesis imperfecta at Military Hospital 7 A.

29p angicungduoc6 21-07-2020 23 2   Download

A field experiment was conducted at Boodamanahalli village, (Bangalore rural district) to know the effect of tikka disease, caused together by Cercospora arachidicola and Cercosporidium personatum on biochemical parameters viz., total sugars, total phenols and total proteins as influenced by different sowing dates in groundnut. It included two varieties, TMV-2 a highly susceptible one and GBPD-4 a resistant with nine sowing dates starting from December 2008 to August 2009 at monthly intervals.

9p chauchaungayxua6 26-06-2020 19 2   Download

India accounts for 30% of neonatal deaths globally. Bacterial sepsis is a significant cause of morbidity and mortality in newborns. The study helps to make antibiotic policy in neonatal sepsis. The main objective is to study the incidence of multidrug resistant gram negative and gram positive organisms causing neonatal septicemia and their antibiotic sensitivity pattern. The study was conducted in the Department of Microbiology over a period of one year. Sample of blood was collected under aseptic precautions and processed by standard techniques.

5p gaocaolon5 14-06-2020 25 3   Download

Source and sink activity measured after anthesis in six poplar rice cultivars. The experiment was laid in randomized block design with six cultivars MTU-1001, MTU1010, MTU-7029, BPT-2231, BPT-3291 and BPT-5204 replicated four times. After anthesis, top three leaves separately, top three internodes collectively, panicle axis and grain of main culm were sampled at seven day interval and analysed for biochemical parameters that serve remobilization of nutrients. During the initial and later halves of the sampling, there was a shift in higher amylase activity from third to flag leaves.

8p gaocaolon5 14-06-2020 10 1   Download

A Total 8 semen sample from four healthy Gir breeding bulls were collected at weekly interval for two weeks period using of artificial vagina method for evaluation of semen biochemical attributes at various stages of cryopreservation of Gir bull semen. The overall mean values for oxidative stress parameters viz. lipid peroxidation and glutathione reductase were 51.14 ± 0.18 μmol/ml and 32.24 ± 0.64 U/L, respectively in neat semen samples.

7p nguaconbaynhay5 16-05-2020 16 0   Download

Trehalose metabolism is an essential component of the stress response in yeast cells. In this work we show that the products of the principal genes involved in trehalose metabolism in Schizosaccharomyces pombe, tps1+ (coding for trehalose6-P synthase, Tps1p), ntp1+ (encoding neutral trehalase, Ntp1p) and tpp1+ (that codes for trehalose-6-P phosphatase, Tpp1p), interact in vitro with each other and with themselves to form protein complexes. Disruption of the gene tps1+ blocks the activation of the neutral trehalase induced by heat shock but not by osmotic stress.

9p system191 01-06-2013 28 4   Download

The transcription factor serum response factor (SRF) regulates expression of growth factor-dependent genes and muscle-specific genes in vertebrates. Homologous factors regulate differentiation of some ectodermic tissues in invertebrates. To explore the molecular basis of these different physiological functions, the functionality of human, Drosophila melanogaster and Artemia franciscana SRFs in mammalian cells has been compared in this article. D. melanogaster and, to a lesser extend, A.

9p system191 01-06-2013 32 5   Download

In this study we describe the isolation of three genes of the CYP11B family of the guinea pig. CYP11B1 codes for the previously described 11b-hydroxylase [Bulow, H.E., Mobius, ¨ ¨ K., Bahr, V. & Bernhardt, R. (1996) Biochem. Biophys. Res. ¨ Commun. 221, 304–312] while CYP11B2 represents the aldosterone synthase gene. As no expression for CYP11B3 was detected this gene might represent a pseudogene.

9p system191 01-06-2013 39 3   Download

Starch defines a semicrystalline polymer made of two different polysaccharide fractions. The A- and B-type crystalline lattices define the distinct structures reported in cereal and tuber starches, respectively. Amylopectin, the major fraction of starch, is thought to be chiefly responsible for this semicrystalline organization while amylose is generally considered as an amorphous polymer with little or no impact on the overall crystalline organization.

11p system191 01-06-2013 45 4   Download

A recombinant lipase cloned from Pseudomonas fragi strain IFO 3458 (PFL) was found to retain significant activity at low temperature. In an attempt to elucidate the structural basis of this behaviour, a model of its three-dimensional structure was built by homology and compared with homologous mesophilic lipases, i.e. the Pseudomonas aeruginosa lipase (45% sequence identity) and Burkholderia cepacia lipase (38%).

8p system191 01-06-2013 48 4   Download

Guanosine triphosphate nucleotide analogues such as GppNHp (also named GMPPNP) or GTPcS are widely used to stabilize rapidly hydrolyzing protein-nucleotide complexes and to investigate biochemical reaction pathways. Here we describe the chemical synthesis of guanosine 5¢-O-(c-amidotriphosphate) (GTPcNH2) and a new synthesis of guanosine 5¢-O-(c-fluorotriphosphate) (GTPcF). The two nucleotides were characterized using NMR spectroscopy and isothermal titration calorimetry. Chemical shift data on 31P, 19F and 1H NMR resonances are tabulated.

9p system191 01-06-2013 32 5   Download

In a previous study, the S100A8/A9 protein, a Ca2+- and arachidonic acid-binding protein, abundant in neutrophil cytosol, was found to potentiate the activation of the redox component of the O2– generating oxidase in neutrophils, namely the membrane-bound flavocytochrome b, by the cytosolic phox proteins p67phox, p47phox and Rac ` (Doussiere J., Bouzidi F. and Vignais P.V. (2001) Biochem. Biophys. Res. Commun. 285, 1317–1320). This led us to check by immunoprecipitation and protein fractionation whether the cytosolic phox proteins could bind to S100A8/A9.

10p system191 01-06-2013 30 4   Download

The expression of mitochondrial and hydrogenosomal ADP/ATP carriers (AACs) from plants, rat and the anaerobic chytridiomycete fungus Neocallimastix spec. L2 in Escherichia coli allows a functional integration of the recombinant proteins into the bacterial cytoplasmic membrane. For AAC1 and AAC2 from rat, apparent Km values of about 40 lM for ADP, and 105 lM or 140 lM, respectively, for ATP have been determined, similar to the data reported for isolated rat mitochondria.

10p system191 01-06-2013 37 3   Download

We report here the full coding sequence of a novel mouse putative membrane-associated mucin containing three extracellular EGF-like motifs and a mucin-like domain consisting of at least 20 tandem repeats of 124–126 amino acids. Screening a cosmid and a BAC libraries allowed to isolate several genomic clones. Genomic and cDNA sequence comparisons showed that the gene consists of 25 exons and 24 introns covering a genomic region of  52 kb. The first intron is  16 kb in length and is followed by an unusually large exon ( 9.5 kb) encoding Ser/Thr-rich tandemly repeated sequences....

10p system191 01-06-2013 45 4   Download

There is extensive evidence that FcR c-chain couples to the collagen receptor glycoprotein VI (GPVI) and becomes phosphorylated on tyrosines upon receptor cross-linking. However, it is not established whether this receptor complex is sufficient to initiate the signalling cascade. We transfected GPVI and the FcR c-chain into the human erythroleukaemia cell line K562, which lacks detectable expression of GPVI and the FcR c-chain. The results show that GPVI is unable to signal when expressed alone, despite its surface expression, upon stimulation with the snake C-type lectin, convulxin....

10p system191 01-06-2013 35 3   Download

Recently, we have identified and purified minisatellite DNA binding proteins (MNBPs) that bind to the mouse hypervariable minisatellite Pc-1, from NIH3T3 cells. This study describes the isolation and characterization of a mouse leucine-rich protein (mLRP130) as one of the MNBPs that binds to the C-rich strand of Pc-1. The mLRP130 cDNA was demonstrated to encode a polypeptide of 1306 aminoacid residues with a deduced molecular mass of 137 kDa, and the mLRP130 mRNA is detected in various organs, including heart, brain, liver, skeletal muscle, kidneys and testes.

7p system191 01-06-2013 25 4   Download

Laboratory for Lipoprotein Chemistry and 4Flanders Interuniversity Institute for Biotechnology, Department of Medical Protein Research, Faculty of Medicine, Department of Biochemistry, Ghent University, Belgium; 2Laboratory of Biomolecular Dynamics, Katholieke Universiteit Leuven, Belgium; 3Ecole Normale Supe´rieure, Paris, France Penetratin is a 16-amino-acid peptide, derived from the homeodomain of antennapedia, a Drosophila transcription factor, which can be used as a vector for the intracellular delivery of peptides or oligonucleotides....

9p system191 01-06-2013 33 4   Download