Chemical modification

The purpose of the thesis is to synthesize the effective catalysts based on Pt/SBA-15 modifed with Al and/or B and their applicability in n-heptane hydroisomerization, tetralin hydrogenation and paracetamol detection.

129p closefriend04 17-10-2021 17 4   Download

The aim of the thesis: Study on chemical constituents of two Knema species including Knema pachycarpa and Knema saxatilis growing in Vietnam. Evaluate cytotoxic and cetylcholinesterase enzyme inhibitory activitiesof isolates to find out bioactive compounds.

25p extraenglish 24-05-2021 19 4   Download

The aim of the thesis: Study on chemical composition of starfish Acanthaster planci of Vietnam, synthesizing hydroxyl and oxime derivatives from a steroid isolated from this starfish and assessing biological activity of isolated and synthesized compounds.

26p extraenglish 24-05-2021 21 4   Download

Study on chemical composition of starfish Acanthaster planci of Vietnam, synthesizing hydroxyl and oxime derivatives from a steroid isolated from this starfish and assessing biological activity of isolated and synthesized compounds.

26p capheviahe26 25-01-2021 10 4   Download

The envelope protein gp64 of the baculovirusAutographa californicanuclear polyhedrosis virus is essential for viral entry into insect cells, as the glycoprotein bothmediates pH-dependentmembrane fusionandbinds tohost cell receptors. Surface modification of baculovirus particles by genetic engineering of gp64 has been demonstrated by various strategies and thus has become an important and powerful tool in molecular biology.

10p research12 23-04-2013 27 2   Download

It was shown previously [Riedinger, H. J., van Betteraey-Nikoleit, M & Probst, H. (2002)Eur. J. Biochem.269, 2383–2393] that initiation ofin vivo SV40 DNA replication is reversibly suppressed by hypoxia in a state where viral minichromosomes exhibit a nearly complete set of repli-cation proteins. Reoxygenation triggers fast completion and post-translational modifications.

11p tumor12 20-04-2013 30 2   Download

Antisense agents are valuable tools to inhibit the expression of a target gene in a sequence-specific manner,and may be used for functional genomics,target validation and thera-peuticpurposes.Three typesof anti-mRNAstrategies canbe distinguished. Firstly,the use of single stranded antisense-oligonucleotides; secondly,the triggering of RNA cleavage through catalytically active oligonucleotides referred to as ribozymes; and thirdly,RNA interference induced by small interfering RNA molecules.

17p fptmusic 16-04-2013 40 2   Download

Mucin type O-glycosylation is a widespread modification of eukaryotic pro-teins. The transfer of N-acetylgalactosamine to selected serine or threonine residues is catalyzed by a family of polypeptide N-acetylgalactosaminyl-transferases localized in the Golgi apparatus. The most abundant elonga-tion of O-glycans is the addition of a b1-3 linked galactose by the core-1 b1-3 galactosyltransferase (core-1b3GalT), thereby building the T-antigen or core-1 structure Gal(b1-3)GalNAc(a1-O).

11p fptmusic 11-04-2013 33 4   Download

We have investigated the molecular mechanisms that produce different structural and functional behavior in the monomeric and trimeric forms of seminal vesicle protein no. 4, a protein with immunomodulatory, anti-inflamma-tory, and procoagulant activity secreted from the rat seminal vesicle epithelium. The monomeric and trimeric forms were characterized in solution by CD.

9p dell39 03-04-2013 35 5   Download

UDP-galactose 4-epimerases from the yeastKluyvero-myces fragilisandEscherichia coliare both homodimers but the molecular mass of the former (75 kDa/subunit) is nearly double that of the latter (39kDa/subunit). Protein databank sequence homology revealed the possibility of mutarotase activity in the excess mass of the yeast enzyme. This was confirmed by three independent assay protocols. With the help of specific inhibitors and chem-ical modification reagents, the catalytic sites of epimerase and mutarotase were shown to be distinct and inde-pendent....

11p dell39 03-04-2013 44 4   Download

High-quality quantitative data generated under standardized conditions is critical for understanding dynamic cellular processes. We report strategies for error reduction, and algorithms for automated data processing and for establishing the widely used techniques of immunoprecipitation and immu-noblotting as highly precise methods for the quantification of protein levels and modifications.

12p dell39 27-03-2013 53 3   Download

Murine Atg8L⁄Apg8L has significant homology with the other known mammalian Atg8 homologs, LC3, GABARAP and GATE-16. However, it is unclear whether murine Atg8L modification is mediated by human Atg4B, Atg7 and Atg3. Expression of Atg8L in HEK293 cells led to clea-vage of its C-terminus.In vitro, the C-terminus of Atg8L was cleaved by human Atg4B, but not human Atg4A or Atg4C.

10p inspiron33 26-03-2013 40 5   Download

Protein glycation by methylglyoxal is a nonenzymatic post-translational modification whereby arginine and lysine side chains form a chemically heterogeneous group of advanced glycation end-products. Methylglyoxal-derived advanced glycation end-products are involved in pathologies such as diabetes and neurodegenerative diseases of the amyloid type.

15p inspiron33 23-03-2013 51 4   Download

The crystal structure of human pancreatic cationic trypsin showed the chemical modification of Tyr154, which was originally described as phos-phorylation [Gaboriaud C, Serre L, Guy-Crotte O, Forest E & Fontecilla-Camps JC (1996)J Mol Biol259, 995–1010]. Here we report that Tyr154 is sulfated, not phosphorylated.

7p inspiron33 23-03-2013 26 3   Download

Cellulose and wheat straw degradation byRuminococcus albuswas moni-tored using NMR spectroscopy. In situ solid-state 13 C-cross-polarization magic angle spinning NMR was used to monitor the modification of the composition and structure of cellulose and 13 C-enriched wheat straw during the growth of the bacterium on these substrates.

9p galaxyss3 07-03-2013 39 3   Download

At the optimal temperature (65C),Thermus thermophiluspolynucleotide phosphorylase (Tth PNPase), produced inEscherichia colicells and isolated to functional homogeneity, completely destroys RNAs that possess even a very stable intramolecular secondary structure, but leaves intact RNAs whose 3¢ end is protected by chemical modification or by hybridization with a complementary oligonucleotide.

13p media19 06-03-2013 43 3   Download

Peptides function as chemical signals between cells of multicellular organ-isms via specific receptors on target cells. Many hormones, neuromodula-tors and growth factors are peptides. Peptide hormones and other biologically active peptides are synthesized as higher molecular weight pre-cursor proteins (pro-hormones), which must undergo post-translational modification to yield the bioactive peptide(s).

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Strategies for the chemical construction of synthetic proteins with precisely positioned post-translational modifications or their mimics offer a powerful method for dissecting the complexity of functional protein alteration and the associated complexity of proteomes.

11p media19 06-03-2013 48 2   Download

Glycosylation is one of the most common post-translational modifications, and approximately 50% of all proteins are presumed to be glycosylated in eukaryotes. Branched N-glycans, such as bisecting GlcNAc,b-1,6-GlcNAc and core fucose (a-1,6-fucose), are enzymatic products of N-acetylglucos-aminyltransferase III,N-acetylglucosaminyltransferase V and a-1,6-fucosyl-transferase, respectively.

10p media19 06-03-2013 32 4   Download

Free nucleotides in living cells play important roles in a variety of biolo-gical reactions, and often undergo chemical modifications of their base moieties. As modified nucleotides may have deleterious effects on cells, they must be eliminated from intracellular nucleotide pools.

13p vinaphone15 27-02-2013 34 3   Download