![](images/graphics/blank.gif)
Chlamydomonas reinhardtii
-
In this study, various physical parameters (temperature, light intensity and UV-C radiation) which could be effective in heat shock response on C.reinhardtii by using molecular tools were investigated. In total, 256 transformants were obtained, among them, 160 transformants had continuous expression while 96 of them had heat-inducible expression. In these transformants, arylsulfatase activities were detected qualitatively and quantitatively.
10p
lyhuyenthu
31-01-2023
5
2
Download
-
In this paper we describe the use of camelid antibody fragments (VHHs) and a streamlined ELISA assay as powerful new tools for obtaining mono-specific reagents for detecting individual algal cell wall components and for isolating algae that share a particular cell surface component.
12p
viminato2711
22-05-2020
6
0
Download
-
The versatile Vacuole Membrane Protein 1 (VMP1) has been previously investigated in six species. It has been shown to be essential in macroautophagy, where it takes part in autophagy initiation.
13p
viminato2711
22-05-2020
13
0
Download
-
The PII proteins constitute a large superfamily, present in all domains of life. Until now, PII proteins research in Chloroplastida (green algae and land plants) has mainly focused on post-translation regulation of these signal transductors.
10p
vishikamaru2711
29-04-2020
13
0
Download
-
Proteasomes remove regulatory proteins in eukaryotic cells, and control a variety of plant processes. Proteasomes are localized to the cytosol and nuclear, but their role in plant biology has recently been extended to chloroplasts, where it regulates TOC complex.
10p
viharuno2711
21-04-2020
11
1
Download
-
In plant-derived animal feedstuffs, nearly 80 % of the total phosphorus content is stored as phytate. However, phytate is poorly digested by monogastric animals such as poultry, swine and fish, as they lack the hydrolytic enzyme phytase; hence it is regarded as a nutritionally inactive compound from a phosphate bioavailability point of view.
7p
viuchiha2711
21-04-2020
17
0
Download
-
Chlamydomonas reinhardtii is an unicellular green alga used for functional genomics studies and heterologous protein expression. A major hindrance in these studies is the low level and instability of expression of nuclear transgenes, due to their rearrangement and/or silencing over time.
12p
vihamax2711
21-04-2020
9
0
Download
-
Trong nghiên cứu này, câc tác giả đã sử dụng kỹ thuật xung điện để chuyển gen vp28 của WSSV vào hệ gen nhân của C. reinhardtii, nhằm tạo ra chủng tảo lục tái tổ hợp biểu hiện protein VP28 như là vaccine đường ăn cho tôm.
8p
shiwo_ding7
05-06-2019
59
3
Download
-
This review provides a brief summary of the current knowledge on oleaginous algae and their fatty acid and TAG biosynthesis, algal model systems and genomic approaches to a better understanding of TAG production, and a historical perspective and path forward for microalgae-based biofuel research and commercialization.
19p
vannhat_xq
25-02-2017
76
4
Download
-
Chapter 14 - Prokaryotic and organelle genetics. This chapter begins with a discussion of the origins of yeast mitochondrial work. It then discusses the development of Chlamydomonas reinhardtii as a model organism. It also shows how early work on the organelle genetics of Chlamydomonas and yeast were carried out relatively independently; but inevitably the rationales soon paralleled one another.
69p
tangtuy04
12-03-2016
53
3
Download
-
Starch defines a semicrystalline polymer made of two different polysaccharide fractions. The A- and B-type crystalline lattices define the distinct structures reported in cereal and tuber starches, respectively. Amylopectin, the major fraction of starch, is thought to be chiefly responsible for this semicrystalline organization while amylose is generally considered as an amorphous polymer with little or no impact on the overall crystalline organization.
11p
system191
01-06-2013
44
3
Download
-
The physiological role of sulfoquinovosyl diacylglycerol (SQDG) in photosynthesis was investigated with a SQDG defective mutant (hf-2) of Chlamydomonas reinhardtii that did not have any detectable amount of SQDG. The mutant showed a lower rate of photosystem II (PSII) activity by 40% and also a lower growth rate than those of the wildtype. Results of genetical analysis of hf-2 strongly suggest that the SQDG defect and the lowered PSII activity are due to a single gene mutation.
6p
research12
01-06-2013
34
3
Download
-
Chlamydomonas reinhardtii, a unicellular green alga, contains a hydrogenase enzyme, which is induced by anaerobic adaptation of the cells. Using the suppression subtractive hybridization (SSH) approach, the differential expression of genes under anaerobiosis was analyzed. A PCR fragment with similarity to the genes of bacterial Fe-hydrogenases was isolated and used to screen an anaerobic cDNA expression library of C. reinhardtii.
11p
research12
01-06-2013
38
4
Download
-
All living organisms contain redox systems involving thior-edoxins (Trx), proteins featuringanextremelyconservedand reactive active site that perform thiol-disul®de interchanges with disul®de bridges of target proteins. In photosynthetic organisms, numerous isoforms of Trx coexist, as revealedby sequencing ofArabidopsisgenome. The speci®c functions of many of them are still unknown.
11p
research12
29-04-2013
33
1
Download
-
We have isolated and characterized a second [Fe]-hydro-genasegene fromthegreenalga,Chlamydomonas reinhardtii. TheHydA2gene encodes a protein of 505 amino acids that is 74% similar and 68% identical to the known HydA1 hydrogenase fromC. reinhardtii.HydA2 contains all the conserved residues and motifs found in the catalytic core of the family of [Fe]-hydrogenases. We demonstrate that both theHydA1and theHydA2transcripts are expressed upon anaerobic induction, achieved either by neutral gas purging or by sulfur deprivation of the cultures. ...
9p
fptmusic
16-04-2013
44
1
Download
-
The composition of the chloroplast-localized protease complex, ClpP, from the green alga Chlamydomonas reinhardtiiwas characterized by nondena-turing electrophoresis, immunoblotting and MS. The detected ClpP complex has a native mass of540 kDa, which is200 kDa higher than ClpP complexes in higher plant chloroplasts, mitochondria or bacteria. The 540-kDa ClpP complex contains two nuclear-encoded ClpP proteins (ClpP3 and P5) and five ClpR (R1, R2, R3, R4 and R6) proteins, as well two pro-teins, ClpP1Land ClpP1H, both probably derived from the plastid clpP1 gene. ...
14p
fptmusic
12-04-2013
42
1
Download
-
The State 1 to State 2 transition in the photosynthetic membranes of plants and green algae involves the functional coupling of phosphorylated light-harvesting complexes of photosystem II (LHCII) to photosystem I (PSI). We present evidence suggesting that inChlamydomonas reinhardtiithis coupling may be aided by a hyper-phosphorylated form of the LHCII-like CP29 protein (Lhcbm4).
10p
fptmusic
12-04-2013
33
1
Download
-
The glyceraldehyde-3-phosphate dehydrogenase (GAPDH) in the chloroplast ofChlamydomonas reinhardtiiis part of a complex that also includes phosphoribulokinase (PRK) and CP12. We identified two residues of GAPDH involved in protein–protein interactions in this complex, by changing residuesK128 andR197 intoAorE.K128A/Emutants had aKmfor NADH that was twice that of the wild type and a lower catalytic constant, whatever the cofactor.
8p
awards
05-04-2013
30
2
Download
-
To test thioredoxin resistance to oxidizing free radicals, we have studied the one-electron oxidation of wild-type thio-redoxin and of two forms with the point mutations D30A and W35A, using azide radicals generated byc-ray or pulse radiolysis. The oxidation patterns of wild-type thioredoxin and D30A are similar. In these forms, Trp35 is the primary target and is repaired by one-electron reduction; first by intramolecular electron transfer from tyrosine, and then fromother residues. Conversely, duringoxidationofW35A, Trp13 is poorly reactive.
7p
awards
05-04-2013
34
3
Download
-
Sulfoquinovosyl diacylglycerol (SQDG) is involved in the maintenance of photosystem II (PSII) activity in Chlamydomonas reinhardtii[Minoda, A., Sato, N., Nozaki, H., Okada, K., Takahashi, H., Sonoike, K. & Tsuzuki, M. et al.(2002) Eur. J. Biochem.269, 2353–2358]. To under-stand the spread of the taxa in which PSII interacts with SQDG, especially in cyanobacteria, we produced a mutant defective in the putativesqdBgene responsible for SQDG synthesis from two cyanobacteria, Synechocystis sp.
9p
dell39
03-04-2013
44
2
Download
CHỦ ĐỀ BẠN MUỐN TÌM
![](images/graphics/blank.gif)