Cloning and adapting

Objectives of the study: Determining the growth characteristics and adaptability of some triploid acacia clones in 3 ecological regions (Northeast, North Central and Southeast); đetermine the basic wood properties for some promising triploid acacia clones; identify some reproductive characteristics of some triploid acacia clones

27p guitaracoustic09 14-01-2022 15 2   Download

Random PCR mutagenesis was applied to theThermus thermophilus xylA gene encoding xylose isomerase. Three cold-adapted mutants were isolated with the following amino-acid substitutions: E372G, V379A (M-1021), E372G, F163L (M-1024) and E372G (M-1026). The wild-type and mutated xylAgenes were cloned and expressed in Escherichia coliHB101 using the vector pGEMÒ-T Easy, and their physicochemical and catalytic properties were determined.

7p research12 29-04-2013 54 3   Download

Thyrotropin-releasing hormone receptor (TRHR) has already been cloned in mammals where thyrotropin-releas-inghormone (TRH) isknowntoactasapowerful stimulator of thyroid-stimulating hormone (TSH) secretion. The TRH receptor of amphibians has not yet been characterized, although TRH is specifically important in the adaptation of skin color to environmental changes via the secretion of a-melanocyte-stimulating hormone (a-MSH).

11p research12 23-04-2013 32 4   Download

Apsychrotrophic bacteriumShewanellasp. strain SIB1 was grown at 4 and 20
C, and total soluble proteins extracted from the cells were analyzed by two-dimensional poly-acrylamide gel electrophoresis. Comparison ofthese pat-terns showed that the cellular content ofa protein with a molecular mass of28 kDa and an isoelectric point of four greatly increased at 4
C compared to that at 20
C.

10p dell39 03-04-2013 45 4   Download

Endonuclease I is a periplasmic or extracellular enzyme present in many different Proteobacteria. TheendAgene encoding endonuclease I from the psychrophilic and mildly halophilic bacteriumVibrio salmonicidaand from the mesophilic brackish water bacterium Vibrio choleraehave been cloned, over-expressed inEscherichia coli, and purified.

12p inspiron33 23-03-2013 57 5   Download