Combination disc method

The study was carried out according to the single-factor experimental method to determine the main working parameters of the aerodynamic sowing unit combined with the inclined disc.The results showed that an inclined seed disc is more effective than a vertical one in receiving and holding seeds during the sowing process.

10p vifalcon 18-05-2023 4 2   Download

This paper proposed solutions for profiling the disc tool in machining a helical cylindrical surface with constant pitch. A section method has been developed in AutoCAD environment together with a Boolean operation. Especially, this work presented a computational problem of determining the any section of a helical surface, while other documents only refer to the section along axis and cross section. The authors used a combinative method of analytics, graphics and programming to solve that problem, applying in designing cutting tool for machining helical cylindrical surfaces.

5p quenchua12 10-05-2021 21 3   Download

Axial loading during MRI (alMRI) combined with T2 mapping recently was shown as a promising method to reveal biomechanical intervertebral disc (IVD) characteristics. This feasibility study aims to investigate whether there is a diurnal variation in the IVD T2-value when using alMRI.

8p vitennessee2711 02-02-2021 5 0   Download

The present study was undertaken with an objective of phenotypic and molecular detection of beta-lactam antimicrobial resistance in E. coli isolated from freshwater fish in Andhra Pradesh.

7p gaocaolon9 22-12-2020 11 2   Download

Extended spectrum beta lactamase producers are posing a major obstacle in the therapeutic outcome of patients. They cause enzymatic hydrolysis of the beta lactum ring, resulting in resistance to penicillins and cephalosporins. The rise in ESBL producers results in prolonged treatment which leads to increased hospital stay and financial burden on the patient. It is necessary for all the hospitals to monitor ESBL producers to formulate preventive and therapeutic measures accordingly. The study was conducted in a tertiary care hospital during the period from December 2015 to November 2016.

7p angicungduoc8 07-11-2020 15 1   Download

Antimicrobial resistance is a growing threat worldwide. Increasing resistance to third generation cephalosporins has become a cause for concern. The prevalence of extended spectrum–lactamases (ESBLs) and metallobetalactamases (MBL) among members of Enterobacteriaceae constitutes a serious threat to current -lactam therapy leading to treatment failure. A total of 276 Gram negative isolates were processed. ESBL was detected by phenotypic confirmatory disc diffusion test (PCDDT) using ceftazidime alone and in combination with clavulanic acid.

8p angicungduoc6 20-07-2020 19 1   Download

Acinetobacter the aerobic non fermenting GNCB are emerging as important pathogens in health care associated infection, exhibiting increased antimicrobial resistance by virtue of multiple resistant mechanisms with practically no therapeutic options. The objective was to determine the multitude of resistant mechanisms by phenotypic and genotypic characterization and the prevalent antimicrobial susceptibility pattern. 175 clinically significant non-duplicate Acinetobacter isolates were included.

10p nguaconbaynhay6 23-06-2020 12 1   Download

The aim of this study was to analyze the extended spectrum of β lactamase (ESBL), metallo β lactamase (MBL) and AmpC production in Pseudomonas aeruginosa in various clinical samples. A Total of 100 clinical isolates of P. aeruginosa were collected from different clinical specimen and confirmed by standard tests. Antibiotic susceptibility was determined by the Kirby-Bauer disc diffusion method. ESBL screening was done using 3rd generation cephalosporins and confirmatory combined double disc test, imipenem-EDTA double disc synergy test for MBL enzyme and AmpC test using Cefoxitin disc.

9p trinhthamhodang1213 30-05-2020 11 1   Download

Non fermenting Gram Negative Bacilli (NFGNB) once considered as contaminants, now emerged as a major cause of life threatening nosocomial infections and as multidrug resistant pathogens. Acinetobacter species are the opportunistic pathogens with increasing prevalance in the nosocomial infections. Community acquired infections are also common in Acinetobacter. It accounts for 10% of all community-acquired bacteremic pneumonias. To isolate, identify and detect Carbapenem resistance producing Acinetobacter spp., and confirm Metallo Beta Lactamase ( MBL) production by phenotypic methods.

10p trinhthamhodang5 16-05-2020 15 1   Download

The present study was done to detect Extended spectrum β lactamase and metallo-βlactamase producing isolates of Pseudomonas aeruginosa from various clinical samples from patients admitted in Tirunelveli Government medical college hospital. In this study we studied the prevalence of ESBL and MBL among 112 Pseudomonas aeruginosa isolates, following standard methods of isolation and identification of these bacteria from clinical materials

8p kethamoi4 18-04-2020 15 0   Download

Resistance to carbapenems is an emerging problem among Klebsiella pneumoniae isolates. A simple and effective phenotypic detection method for carbapenemase production is required in health care settings. A total of 100 clinical isolates of Klebsiella pneumoniae from various samples were screened for imipenem resistance. The imipenem resistant strains were further tested for metallo-beta-lactamase (MBL) production by imipenem combined disc test and ceftazidime combined disc test.

6p kethamoi4 16-04-2020 8 1   Download

In the present study 105 numbers of microbial isolates were isolated from 96 milk samples and were processed as per routine method of microbial isolation. Various types of tea extracts like black tea and green tea were used in combination with antibiotic discs obtained from HiMedia, Mumbai. The synergistic effects of antibiotics and tea extracts against resistant bovine pathogens lead to new choices for development of combination therapy during chronic mastitis.

7p caygaocaolon2 11-03-2020 17 4   Download

Metallo beta-lactamase (MBL) and biofilm production is the most worrisome resistant mechanisms observed in Pseudomonas aeruginosa. Increased resistance of Pseudomonas to antibiotic therapy is an emerging global health concern which can be overcomed by Silver nanoparticles (AgNPs). Therefore this study aims to evaluate the antibacterial and antibiofilm activity of Silver nanoparticles on MBL and biofilm producing P. aeruginosa isolates using Transmission Electron microscope (TEM) and Confocal laser scanning microscope (CLSM) respectively. A total 40 isolates of p.

18p chauchaungayxua3 07-02-2020 11 0   Download

Neonatal calf diarrhoea (NCD) is a major threat to dairy industries. It is a multi-etiology disease with E. coli as the predominant cause. Emergence of antimicrobial resistance is of utmost concern because it has rendered the present day antibiotics less effective. The present study aims for isolation, molecular characterization and antimicrobial resistant profiling of E. coli from neonatal calves. One hundred two fecal samples were collected from neonatal calves for isolation of E. coli. All the isolates showed positive result on biochemical analysis. But only 78.

12p chauchaungayxua3 07-02-2020 24 0   Download

One of the common causes of morbidity and mortality following burn injury is infections. The emergence of metallo-β-lactamase (MBL) in Gram negative bacilli (GNB) has reduced the available therapeutic options for effective treatment. Therefore, for screening of carbapenemase producers, a simple and inexpensive testing method is essential. To know the prevalence of MBL production in various gram negative isolates, to evaluate different phenotypic methods to detect MBL production and to find out antibiotic sensitivity profile of MBL producing gram negative bacilli.

9p quenchua2 15-12-2019 14 0   Download

Carbapenem antibiotics are very often used against multidrug resistant strains clinically troublesome pathogens which developed and proved that the resistance and metallo-βlactamases (MBL) production were a disaster in treating infections. The identification and detection of MBL-producing bacterial strains were having crucial importance for the prevention of nosocomial infections. Therefore the present study was undertaken for screening MBL production Gram Negative bacteria.

8p quenchua2 15-12-2019 13 1   Download

Non-fermenting gram-negative bacilli (NFGNB) are an emerging problem in Blood stream infections. A major concern is multi-drug resistance which severely limits treatment options. Earlier it was believed to be non pathogenic, but recently they are more frequently isolated as primary pathogen. Usually they cause hospital acquired infection (HAI). A prospective study was conducted to isolate the NFGNB from blood samples, to identify the risk factors leading to blood stream infections and to determine the antibiotic susceptibility pattern of them.

14p nguaconbaynhay1 04-12-2019 17 1   Download