Conserved domain

The objective of this study was to analyze the features of FtsZ proteins in the Bacillus and Lactococcus genus groups. By exploring the available genomes, we identified and characterized FtsZ proteins in 19 Bacillus and 22 Lactococcus species.

12p vibecca 01-10-2024 1 1   Download

Regulator of G-protein signaling (RGS) proteins were originally identified as negative regulators of G-protein-coupled receptor (GPCR) signaling via their GTPase-accelerating protein (GAP) activity. All RGS proteins contain evolutionarily conserved RGS domain; however, they differ in their size and regulatory domains. RGS1 and RGS10 are smaller than other RGS proteins, and their functions involve various inflammatory responses including autoimmune responses in both the periphery and the central nervous system (CNS). Neuroinflammation is the chronic inflammatory response in the CNS.

5p caothientrangnguyen 09-05-2020 24 1   Download

Lecture Digital image processing - Spatial and frequency domain filter design include all of the following content: Spatial domain filter design (average/mean filter, median filter, gaussian smoothing, conservative smoothing), frequency – based filter design.

10p youcanletgo_04 17-01-2016 44 2   Download

The transcription factor serum response factor (SRF) regulates expression of growth factor-dependent genes and muscle-specific genes in vertebrates. Homologous factors regulate differentiation of some ectodermic tissues in invertebrates. To explore the molecular basis of these different physiological functions, the functionality of human, Drosophila melanogaster and Artemia franciscana SRFs in mammalian cells has been compared in this article. D. melanogaster and, to a lesser extend, A.

9p system191 01-06-2013 32 5   Download

The human immunodeficiency virus type 1, HIV-1, genome encodes a highly conserved regulatory gene product, Vpr (96 amino acids), which is incorporated into virions in quantities equivalent to those of the viral Gag protein. In infected cells, Vpr is believed to function during the early stages of HIV-1 replication (such as transcription of the proviral genome and migration of preintegration nuclear complex), blocks cells in G2 phase and triggers apoptosis.

10p system191 01-06-2013 44 3   Download

The centromere–kinetochore complex is a highly specialized chromatin domain that both mediates and monitors chromosome–spindle interactions responsible for accurate partitioning of sister chromatids to daughter cells. Centromeres are distinguished from adjacent chromatin by specific patterns of histone modification and the presence of a centromere-specific histone H3 variant (e.g. CENP-A). Centromere-proximal regions usually correspond to sites of avid and persistent sister chromatid cohesion mediated by the conserved cohesin complex.

15p research12 01-06-2013 49 4   Download

A technique has been developed to search a proteome database for new members of a functional class of membrane protein. It takes advantage of the highly conserved secondary structure of functionally related membrane proteins. Such proteins typically have the same number of transmembrane domains located at similar relative positions in their polypeptide sequence. This gives rise to a characteristic pattern of peaks in their hydropathy profiles.

7p research12 01-06-2013 32 4   Download

Ligand binding by the aryl hydrocarbon receptor (AhR), a member of the bHLH-PAS family of transcriptional reg-ulatory proteins, has been mapped to a region within the second ÔPASÕ domain, a conserved sequence motif ®rst discovered in the Per-ARNT-Sim family of proteins.

6p research12 29-04-2013 43 3   Download

Theb1,3-glycosyltransferase enzymes identi®ed to date share several conserved regions and conserved cysteine res-idues, all being located in the putative catalytic domain. To investigate the importance of these motifs and cysteines for the enzymatic activity, 14 mutants of the murine b1,3-galactosyltransferase-I gene were constructed and expressed in Sf9 insect cells. Seven mutations abolished the galacto-syltransferase activity.

7p research12 29-04-2013 38 3   Download

Protein phosphatase 2A (PP2A) is an abundant heterotri-meric serine/threonine phosphatase containing highly con-served structural (A) and catalytic (C) subunits. Its diverse functions in the cell are determined by its association with a highly variable regulatory and targeting B subunit. At least three distinct gene families encoding B subunits are known: B/B55/CDC55, B¢/B56/RTS1 and B¢¢/PR72/130. No homologyhasbeen identi®edamong theBfamilies, and little is knownabout howtheseBsubunits interactwith the PP2A A and C subunits....

7p research12 23-04-2013 29 3   Download

Previous structural andmutagenesis studies indicate that the invariant a-amino and a-carboxyl groups of glutamate receptor agonists are engaged in polar interactions with oppositely charged, conserved arginine and glutamate resi-dues in the ligand-binding domain ofa-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor.To examine the role of these residues (R507 and E727 in the GluR-D subunit) in the discrimination between agonists and anta-gonists, we analyzed the ligand-binding properties of homomeric GluR-D and its soluble ligand-binding domain withmutations at thesepositions....

10p tumor12 22-04-2013 38 3   Download

Thefushi tarazu factor 1 (FTZ-F1) nuclear receptor sub-family comprises orphan receptors with crucial roles in development and sexual differentiation in vertebrates and invertebrates. We describe the structure and functional properties of an FTZ-F1 from the platyhelminth parasite of humans,Schistosoma mansoni, the first receptor from this family tobe characterized inaLophotrochozoan. It contains awell conservedDNA-binding domain (55–63%identity to other family members) and a poorly conserved ligand-binding domain (20% identity to that of zebrafish FF1a)....

12p tumor12 22-04-2013 44 2   Download

Era is a highly conserved GTPase essential for bacterial growth. The N-terminal part of Era contains a conserved GTPase domain, whereas the C-terminal part of the protein contains anRNA- andmembrane-binding domain, theKH domain. To investigate whether the binding of Era to 16S rRNA and membrane requires its GTPase activity and whether the GTPase domain is essential for these acti-vities, the N- and C-terminal parts of the Streptococ-cus pneumoniaeEra –Era-N(aminoacids 1–185) andEra-C (amino acids 141–299), respectively – were expressed and purified. ...

9p tumor12 20-04-2013 32 3   Download

The dermal glands of frogs produce antimicrobial peptides that protect the skin against noxious microorganisms and assist in wound repair. The sequences of these peptides are very dissimilar, both within and between species, so that the 5000 living anuran frogs may produce
100 000 different antimicrobial peptides.

14p fptmusic 16-04-2013 41 2   Download

A conserved putative dimerization GxxxG motif located in the unique membrane-spanning segment of the ATP syn-thase subunit e was altered in yeast both by insertion of an alanine residue and by replacement of glycine by leucine residues. These alterations led to the loss of subunit gand the loss of dimeric and oligomeric forms of the yeast ATP syn-thase. Furthermore,as in null mutants devoid of either subunit e or subunit g,mitochondria displayed anomalous morphologies with onion-like structures. ...

10p fptmusic 16-04-2013 46 2   Download

Caspases are a conserved family of cysteine proteases. They play diverse roles in inflammatory responses and apoptotic pathways. Among the casp-ases is a subgroup whose primary function is to initiate apoptosis. Within their long prodomains, caspases-2, -9 and -12 contain a caspase activation and recruitment domain while caspases-8 and -10 bear death effector domains.

18p fptmusic 12-04-2013 42 2   Download

Oxysterol binding protein (OSBP) and its homologs have been shown to regulate lipid metabolism and vesicular transport. However, the exact molecular function of individual OSBP homologs remains uncharacterized. Here we demonstrate that the yeast OSBP homolog, Osh6p, bound phos-phatidic acid and phosphoinositides via its N-terminal half containing the conserved OSBP-related domain (ORD). Using a green fluorescent protein fusion chimera, Osh6p was found to localize to the cytosol and patch-like or punctate structures in the vicinity of the plasma membrane....

13p fptmusic 12-04-2013 52 3   Download

In humans, eight types of histone H1 exist (H1.1–H1.5, H1
, H1t and H1oo), all consisting of a highly conserved globular domain and less con-served N- and C-terminal tails. Although the precise functions of these iso-forms are not yet understood, and H1 subtypes have been found to be dispensable for mammalian development, it is now clear that specific func-tions may be assigned to certain individual H1 subtypes.

11p fptmusic 11-04-2013 25 1   Download

Class III adenylyl cyclases usually possess six highly conserved catalytic residues. Deviations in these canonical amino acids are observed in several putative adenylyl cyclase genes as apparent in several bacterial genomes. This suggests that a variety of catalytic mechanisms may actually exist. The geneRv0386from Mycobacterium tuberculosiscodes for an adenylyl cyclase catalytic domain fused to an AAA-ATPase and a helix-turn-helix DNA-binding domain.

8p fptmusic 11-04-2013 26 3   Download

Sixgenes are homologs ofDrosophila sine oculisand encode transcription factors that are characterized by a conserved Six domain and homeo-domain. Of the six family members (Six1–Six6) in mice, Six1and Six4 show similar expression patterns during embryogenesis. Six1 –⁄ – mice show defective formation of various organs such as inner ear, nose, skeletal mus-cle, kidney and thymus, whereasSix4 –⁄ – mice show little anomaly in organo-genesis.

16p awards 06-04-2013 28 2   Download