Cyclic AMP

cAMP receptor protein (CRP), allosterically activated by cAMP, regulates the expression of several genes inEscheri-chia coli. As binding of cAMP leads to undefined conform-ational changes in CRP, we performed a steady-state and time-resolved fluorescence study to showhow the binding of the ligand influences the structure and dynamics of the protein. We used CRP mutants containing a single trypto-phan residue at position 85 or 13, and fluorescently labeled with 1,5-I-AEDANS attached toCys178.

11p tumor12 20-04-2013 34 3   Download

Cyclic AMP-dependent protein kinase (PKA) is a holoenzyme that consists of a regulatory (R) subunit dimer and two catalytic (C) subunits that are released upon stimulation by cAMP. Immunoblotting and immunoprecipita-tion of T-cell protein extracts, immunofluorescence of permeabilized T cells and RT⁄PCR of T-cell RNA using C subunit-specific primers revealed expression of two catalytically active PKA C subunits Ca1 (40 kDa) and Cb2 (47 kDa) in these cells.

0p awards 06-04-2013 39 3   Download

Cyclic AMP receptor protein (CRP) regulates the expression of more then 100 genes inEscherichia coli. It is known that the allosteric activation of CRP by cAMP involves a long-distance signal transmission from the N-ter-minal cAMP-binding domain to the C-terminal domain of CRP responsible for the interactions with specific sequences of DNA. In this report we have used a CRP mutant containing a single Trp13 located in the N-terminal domain of the protein.

14p awards 05-04-2013 45 4   Download

In our continued studies on corticotropin releasing factor receptor (CRFR1) signaling in the skin, we tested functional activity of CRFR1a, e, f, g and h isoforms after transfection to COS cells.Both membrane-bound and soluble variants are translatedin vivointofinal proteinproducts that undergo further post-translational modifications.

10p dell39 03-04-2013 45 3   Download

Cyclic AMP responsive element (CRE)-binding protein (CREB) is known to activate transcription when its Ser133 is phosphorylated. Two independ-ent investigations have suggested the presence of Ser133-independent acti-vation. One study identified a kinase, salt-inducible kinase (SIK), which repressed CREB; the other isolated a novel CREB-specific coactivator, transducer of regulated CREB activity (TORC), which upregulated CREB activity.

19p dell39 27-03-2013 43 4   Download

We have investigated the direct effect of arachidonic acid on cholesterol transport in intact cells or isolated mitochondria from steroidogenic cells and the effect of cyclic-AMP on the specific release of this fatty acid inside the mitochondria. We show for the first time that cyclic-AMP can regulate the release of arachidonic acid in a specialized compartment of MA-10 Leydig cells, e.g.

11p inspiron33 23-03-2013 36 5   Download

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Critical Care giúp cho các bạn có thêm kiến thức về ngành y học đề tài: Aspergillus fumigatus allergen expression is coordinately regulated in response to hydrogen peroxide and cyclic AMP...

11p coxanh_5 28-10-2011 72 9   Download

Nhiều hormon tác động đến tế bào đích của chúng thống qua tác động dẫn đến quá trình hình thánh các 3',5'-adenosine moniphosphate (cyclic AMP : AMP vòng). Sau khi được tổng hợp, cAMP sẽ tác động cho các ảnh hưởng của hormon bên trong tế bào. Như vậy, cAMP sẽ đóng vai trò là chất tac động trung gian của hormon trong tế bào và còn được gọi là chất truyền thông tin thứ hai (second mesenger)cho các tác động của hormon. ...

5p heoxinhkute5 16-11-2010 607 19   Download