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Báo cáo khoa học: Identification and characterization of novel PKA holoenzymes in human T lymphocytes

Chia sẻ: Nguyen Thang | Ngày: | Loại File: PDF | Số trang:0

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Cyclic AMP-dependent protein kinase (PKA) is a holoenzyme that consists of a regulatory (R) subunit dimer and two catalytic (C) subunits that are released upon stimulation by cAMP. Immunoblotting and immunoprecipita-tion of T-cell protein extracts, immunofluorescence of permeabilized T cells and RT⁄PCR of T-cell RNA using C subunit-specific primers revealed expression of two catalytically active PKA C subunits Ca1 (40 kDa) and Cb2 (47 kDa) in these cells.

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Nội dung Text: Báo cáo khoa học: Identification and characterization of novel PKA holoenzymes in human T lymphocytes

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