Cytochrome b

Luận án "Một số đặc điểm di truyền ở mức độ phân tử của 15 giống lợn nội Việt Nam" được hoàn thành với mục tiêu nhằm xác định được đa dạng di truyền, khoảng cách di truyền và cấu trúc di truyền của 15 giống lợn nội dựa trên 19 chỉ thị microsatellite. Xác định được mối quan hệ phát sinh loài của 15 giống lợn nội thông qua đa hình trình tự gen Cytochrome B ty thể.

27p conmeothayxao 28-12-2023 8 4   Download

Đồ án tốt nghiệp: Nghiên cứu mã vạch di truyền cá Tra (Pangasianodon hypopthalmus) bằng chỉ thị sinh học phân tử cytochrome b được thực hiện với mục tiêu nhằm phân loại chính xác cá Tra (P. hypophthalmus); xác định được bộ chỉ thị phân tử cytochrome b nhằm kiểm định cá Tra (P. hypophthalmus) có độ tin cậy > 95%. Mời các bạn cùng tham khảo.

64p zhangyan 13-07-2021 35 7   Download

Trong Báo cáo khoa học: Phân biệt thịt trâu và thịt bò bằng kỹ thuật PCR, kỹ thuật PCR được ứng dụng để phân biệt thịt bò và thịt trâu với mục tiêu xây dựng hai quy trình PCR-bò và PCR-trâu. Mồi xuôi (F2) được thiết kế chung cho hai quy trình dựa vào vùng tương đồng trên gen cytochrome b của bò và trâu để giảm chi phí. Còn mồi ngược RC2 (PCR-bò) và RBU2 (PCR-trâu) được thiết kế dựa vào vùng không tương đồng trên gen cytochrome b của bò và trâu. Kết quả thu được PCR-bò, PCR-trâu khuếch đại sản phẩm có kích thước 839bp và 763bp.

12p benben121 31-10-2014 126 5   Download

Trình tự cytochrome B của sao la (Pseudoryx nghetinhensis)

7p banhnamdua 25-07-2013 34 1   Download

Introducing site-directed mutations in surface-exposed residues of subunit II of the heme aa3 cytochrome c oxidase of Paracoccus denitrificans, we analyze the kinetic parameters of electron transfer from reduced horse heart cytochrome c. Specifically we address the following issues: (a) which residues on oxidase contribute to the docking site for cytochrome c, (b) is an aromatic side chain required for electron entry from cytochrome c, and (c) what is the molecular basis for the previously observed biphasic reaction kinetics....

9p system191 01-06-2013 37 3   Download

The Na+-translocating NADH:quinone oxidoreductase (Na+-NQR) from Vibrio alginolyticus was inactivated by reactive oxygen species. Highest Na+-NQR activity was observed in anaerobically prepared membranes that exhibited 1 : 1 coupling of NADH oxidation and Q reduction activities (1.6 UÆmg)1). Optical and EPR spectroscopy documented the presence of b-type cytochromes, a [2Fe)2S] cluster and an organic radical signal in anaerobically pre- pared membranes from V. alginolyticus.

6p research12 01-06-2013 48 4   Download

Conservedphenylalanine 35 is one of the hydrophobic patch residues on the surface of cytochromeb5 (cytb5). This patch is partially exposed on the surface of cyt b5while its buried face is in direct van der Waals’contact with heme b. Resi-dues Phe35 and Phe/Tyr74 also form an aromatic channel with His39, which is one of the axial ligands of heme b. By site-directedmutagenesiswehaveproduced threemutantsof cytb5:Phe35fiTyr, Phe35fiLeu, and Phe35fiHis.

10p research12 23-04-2013 39 3   Download

Human cytochrome P4501A1 (CYP1A1) is one of the key enzymes in the bioactivation of environmental pollutants such as benzo[a]pyrene (B[a]P) and other polycyclic aro-matic hydrocarbons. To evaluate the effect of membrane properties and distinct phospholipids on the activity of human CYP1A1 puri®ed insect cell-expressed human CYP1A1 and of human NADPH-P450 reductase were reconstituted into phospholipid vesicle membranes.

7p research12 23-04-2013 35 2   Download

The heme of cytochrome P460 ofNitrosomonas europaea, which is covalently crosslinked to two cysteines of the polypeptideaswithallc-type cytochromes, has anadditional novel covalent crosslink to lysine 70 of the polypeptide [Arciero,D.M.&Hooper,A.B. (1997)FEBSLett.410, 457– 460]. The protein can catalyze the oxidation of hydroxyl-amine. The gene for this protein,cyp,wasexpressedin Pseudomonas aeruginosastrain PAOlacI, resulting in for-mation of a holo-cytochrome P460 which closely resembled native cytochrome P460 purified fromN. ...

7p fptmusic 16-04-2013 53 4   Download

Whereas the majority of disease-related mitochondrial DNA mutations exhibit significant biochemical and clinical heterogeneity, mutations within the mitochondrially encoded human cytochrome b gene (MTCYB) are almost exclusively associated with isolated complex III deficiency in muscle and a clinical presentation involving exercise intolerance.

10p fptmusic 11-04-2013 36 3   Download

Cellobiose dehydrogenase (CDH) is an extracellular flavocytochrome con-taining flavin and b-type heme, and plays a key role in cellulose degrada-tion by filamentous fungi. To investigate intermolecular electron transfer from CDH to cytochrome c, Phe166, which is located in the cytochrome domain and approaches one of propionates of heme, was mutated to Tyr, and the thermodynamic and kinetic properties of the mutant (F166Y) were compared with those of the wild-type (WT) enzyme.

0p awards 06-04-2013 36 2   Download

Saccharomyces cerevisiaehas been used as a model system to characterize the effect of cytochromebmutations found in fungal and oomycete plant pathogens resistant to Qo inhibitors (QoIs), including the strobilurins, now widely employed in agriculture to control such diseases. Specific residues in the Qosite of yeast cytochromebwere modified to obtain four new forms mimicking the Qobinding site of Erysiphe graminis, Venturia inaequalis, Sphaerotheca fuligi-neaandPhytophthoramegasperma.

8p dell39 03-04-2013 36 3   Download

The mitochondrial cytochromeb missense mutation, G167E, has been reported inapatientwithcardiomyopathy. The residue G167 is located in an extramembranous helix close to the hinge region of the iron–sulfur protein. In order to characterize the effects of the mutation on the structure and function of thebc1complex, we introducedG167E into the highly similar yeast cytochromeb. The mutation had a severe effect on the respiratory function, with the activity of thebc1complex decreased to a fewper cent of the wild type....

7p dell39 03-04-2013 46 5   Download

The cytochromebc1 complex of the yeastSaccharomyces cerevisiae is composed of 10 different subunits that are assembledas a symmetrical dimer in the innermitochondrial membrane. Three of the subunits contain redox centers and participate in catalysis, whereas little is known about the function of the seven supernumerary subunits.

10p dell39 03-04-2013 61 4   Download

Cytochromesb561 are a family of transmembrane proteins found in most eukaryotic cells. Three evolutionarily closely related mammalian cyto-chromes b561 (chromaffin granule cytochrome b, duodenal cytochrome b, and lysosomal cytochromeb) were expressed in a Saccharomyces cerevisiae Dfre1Dfre2 mutant, which lacks almost all of its plasma membrane ferrire-ductase activity, to study their ability to reduce ferric iron (Fe 3+ ).

13p inspiron33 25-03-2013 31 4   Download

The protonation state of residues around the Qo binding site of the cyto-chrome bc1 complex from Paracoccus denitrificansand their interaction with bound quinone(s) was studied by a combined electrochemical and FTIR difference spectroscopic approach. Site-directed mutations of two groups of conserved residues were investigated: (a) acidic side chains located close to the surface and thought to participate in a water chain leading up to the heme bLedge, and (b) residues located in the vicinity of this site. ...

13p inspiron33 23-03-2013 42 5   Download

CYP7B1, a cytochrome P450 enzyme, metabolizes several steroids involved in hormonal signaling including 5a-androstane-3b,17b-diol (3b-Adiol), an estrogen receptor agonist, and dehydroepiandrosterone, a precursor for sex hormones. Previous studies have suggested that CYP7B1-dependent metab-olism involving dehydroepiandrosterone or 3b-Adiol may play an impor-tant role for estrogen receptor b-mediated signaling.

12p media19 06-03-2013 42 2   Download

Tuyển tập các báo cáo nghiên cứu về sinh học được đăng trên tạp chí sinh học thế giới đề tài: Estimating the frequency of Asian cytochrome B haplotypes in standard European and local Spanish pig breeds

8p toshiba19 08-11-2011 81 4   Download

Mỗi phân tử oxy được phát hành bởi các phản ứng ánh sáng nguồn cung cấp trong 4 điện tử cần thiết để làm cho 2 phân tử NADPH.Các chemiosmosis thúc đẩy bởi những 4 electron khi đi qua cytochrome b 6 / f phức tạp chỉ giải phóng đủ năng lượng để bơm 12 proton vào bên trong của thylakoid này.

6p heoxinhkute13 27-04-2011 101 7   Download