DNA fragments

Male reproductive health has experienced a substantial global decline. This meta-analysis was conducted to investigate the influence of different dietary patterns on male reproductive function based on semen parameters, sperm DNA fragmentation, and sex hormones.

13p viharuno 03-01-2025 1 1   Download

Methicillin-resistant Staphylococcus aureus (MRSA) is one of the most predominant agents that cause nosocomial infections. Objectives: To determine the rate of MRSA and the molecular characteristics of the coagulase encoding gene of these isolates based on polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP).

6p viuchiha 03-01-2025 1 1   Download

Assessment of genotype diversity was studied using released and in pipeline genotypes of wheat, of these 22 were released and 14 were in pipeline . Genetic diversity among 36 wheat genotypes was studied using Random amplified polymorphic DNA (RAPD) analysis. 21 RAPD primers (RPI primers) were used for screening 36 wheat genotypes from which 2,868 fragments were amplified. It was observed that 63.3% bands were polymorphic and 36.4% were monomorphic. The percent of polymorphic bands in banding pattern was calculated and it was highest in RPI-22 (81.3%) while lowest was recorded in RPI-2 (33.

8p chauchaungayxua6 26-06-2020 15 3   Download

Kỹ thuật RFLP là kỹ thuật nghiên cứu tính đa hình chiều dài của các phân đoạn DNA dựa trên điểm cắt các enzim giới hạn. Khi ủ DNA với enzim giới hạn ở dung dịch đệm thích hợp ở pH, nhiệt độ thích hợp sẽ sẽ tạo ra những phân đoạn DNA với kích thước khác nhau, từ đó lập nên các bản đồ gen. Tham khảo chương hai "Kỹ thuật RFLP - Restriction fragment length polymorphisms" để hiểu hơn về vấn đề này.

10p thanhbinh2305 09-11-2015 391 65   Download

The insect ecdysteroid receptor consists of a heterodimer between EcR and the RXR-orthologue, USP. We addressed the question of whether this heterodimer, like all other RXR heterodimers, may be formed in the absence of ligand and whether ligand promotes dimerization. We found that C-terminal protein fragments that comprised the ligand binding, but not the DNA binding domain of EcR and USP and which were equipped with the activation or DNA binding region of GAL4, respectively, exhibit a weak ability to interact spontaneously with each other.

9p system191 01-06-2013 30 3   Download

Binding of lamin B receptor (LBR) to chromatin was studied by means of an in vitro assay system involving recombinant fragments of human LBR and Xenopus sperm chromatin. Glutathione-S-transferase (GST)-fused proteins including LBR fragments containing the N-terminal region (residues 1–53) and arginine-serine repeat-containing region (residues 54–89) bound to chromatin. The binding of GST-fusion proteins incorporating the N-terminal and arginine-serine repeat-containing regions to chromatin was suppressed by mild trypsinization of the chromatin and by pretreatment with a DNA solution....

11p research12 01-06-2013 30 4   Download

Thea3b1 integrin is an adhesion receptor for extracellular matrix proteins including isoforms of laminin, and the changes of its expression level in various cancer cells are thought to cause their malignant phenotypes. We have cloned an approximately 4 kb DNA fragment of the 5¢-flanking region of the murinea3 integrin gene and analyzed its promoter activity.

9p research12 23-04-2013 36 3   Download

Methyl-CpG-binding protein 2 (MeCP2) is a multifunc-tional protein involved in chromatin organization and silencing of methylated DNA. MAR-BD, a 125-amino-acid residue domain of chicken MeCP2 (cMeCP2, origin-ally named ARBP), is the minimal protein fragment required to recognize MAR elements and mouse satellite DNA. Here we report the solution structure of MAR-BD as determined by multidimensional heteronuclear NMR spectroscopy.

8p tumor12 20-04-2013 36 2   Download

Sequence analysis of aPaenibacillussp. BP-23 recombinant clone coding for a previously described endoglucanase revealed the presence of an additional truncated ORF with homology to family 48 glycosyl hydrolases. The corres-ponding 3509-bp DNA fragment was isolated after gene walking and cloned inEscherichia coliXl1-Blue for expres-sion and purification. The encoded enzyme, a cellulase of 1091 amino acids with a deduced molecular mass of 118 kDa and a pI of 4.

7p fptmusic 16-04-2013 51 3   Download

We report on a sequence-specific double-stranded DNA labelling strategy in which a stem–loop triplex forming oligonucleotide (TFO) is able to encircle its DNA target. Ligation of this TFO to either a short hairpin oligonucleotide or a long double-stranded DNA fragment leads to the for-mation of a topological complex. This process requires the hybridization of both extremities of the TFO to each other on a few base pairs. The effects of different factors on the formation of these complexes have been investi-gated. ...

10p fptmusic 12-04-2013 32 2   Download

We have determined the nucleotide sequence of a DNA fragment covering the flanking region of the R-stereoselective amidase gene, ramA, from the Pseudomonassp. MCI3434 genome and found an additional gene, bapA, coding for a protein showing sequence similarity to DmpA aminopeptidase from Ochrobactrum anthropiLMG7991 (43% identity).

10p fptmusic 11-04-2013 32 3   Download

Pulchellin is a type 2 ribosome-inactivating protein isolated from seeds of the Abrus pulchellus tenuiflorusplant. This study aims to obtain active and homogeneous protein for structural and biological studies that will clarify the functional aspects of this toxin. The DNA fragment encoding pulchellin A-chain was cloned and inserted into pGEX-5X to express the recombinant pulchellin A-chain (rPAC) as a fusion protein in Escherichia coli. The deduced amino acid sequence analyses of the rPAC presented a high sequential identity ( 86%) with the A-chain of abrin-c. ...

0p awards 05-04-2013 40 3   Download

Folding of DNA into chromatin is mediated by binding to histones such as H4; association of DNA with histones is regulated by covalent histone modifications,e.g. acetylation, methylation,andbiotinylation.We sought to identifyamino-acid residues that are biotinylated in histone H4,and to determine whether acetylation and methylation of histones affect biotinylation. Synthetic peptides spanning fragments of human histone H4 were biotinylated enzymatically using biotinidase. Peptide-bound biotin was probed with strept-avidin–peroxidase....

7p dell39 03-04-2013 37 4   Download

The Hitachi SV1100 utilizes capillary electrophoresis on a microchip that is capable of rapidly sizing DNAfrag-ments. Reproducibility of electrophoresis in different channels was shown by comparing the migration times of the internal controls, DNAfragments of 100 and 800 bp. The range of DNAsizing for this microchip is between 100 and 800 bp, and accuracy in sizing of a 322 bp DNA fragment of a pUC118 PvuII digest was observed, inde-pendent of DNAconcentration. Although relatively good quantification of this fragment was observed with a DNA concentration of 1.

7p dell39 03-04-2013 41 4   Download

Neuronal PAS domain protein 2 (NPAS2) is a circadian rhythm-associated transcription factor with two heme-binding sites on two PAS domains. In the present study, we compared the optical absorption spectra, resonance Raman spectra, heme-binding kinetics and DNA-binding characteristics of the isolated fragment containing the N-terminal basic helix–loop–helix (bHLH) of the first PAS (PAS-A) domain of NPAS2 with those of the PAS-A domain alone.

12p inspiron33 26-03-2013 44 4   Download

Restriction landmark genomic scanning (RLGS) is a powerful method for the systematic detection of genetic mutations in DNA length and epigenetic alteration due to DNA methylation. However, the identification of poly-morphic spots is difficult because the resulting RLGS spots contain very little target DNA and many non-labeled DNA fragments.

9p media19 06-03-2013 35 2   Download

Junction ribonuclease (JRNase) recognizes the transition from RNA to DNA of an RNA–DNA⁄DNA hybrid, such as an Okazaki fragment, and cleaves it, leaving a mono-ribonucleotide at the 5¢ terminus of the RNA– DNA junction. Although this JRNase activity was originally reported in calf RNase H2, some other RNases H have recently been suggested to pos-sess it.

12p vinaphone15 28-02-2013 26 3   Download

Cell death-inducing DFF45-like effector (CIDE) family proteins, including cell death-inducing DFF45-like effector A (CIDEA), cell death-inducing DFF45-like effector B (CIDEB) and cell death-inducing DFF45-like effec-tor C (CIDEC) [fat-specific protein of 27 kDa in rodent (FSP27) in rodents], were originally identified by their sequence homology to the N-terminal region of DNA fragmentation factor DFF40⁄45.

11p viettel02 19-02-2013 33 3   Download

DNA fragmentation is a hallmark of apoptosis that occurs in a variety of cell types; however, it remains unclear whether caspase-3 is required for its induction. To investigate this, we produced caspase-3 knockout Chinese hamster ovary (CHO)-K1 cells and examined the effects of gene knockout and treatment with caspase-3 inhibitors.

9p mobifone23 18-01-2013 35 4   Download

Kỹ thuật AFLP được phát triển bởi công ty KeyGene trong đầu những năm 1990 và đã trở thành một trong những công nghệ in dấu vân tay di truyền (fingerprinting) phổ biến nhất trên toàn thế giới. Kỹ thuật AFLP dựa trên nguyên lý kết hợp giữa kỹ thuật RFLP (Restriction Fragment Length Polymorphism) và PCR để khuếch đại những đoạn DNA có chiều dài giới hạn sau khi được cắt hạn chế bởi enzyme cắt hạn chế. Phương pháp có độ nhạy cao trong việc tìm dấu vết của DNA của bất kỳ nguồn gốc nào hay sự phức tạp nào...

20p nguyenbaotrieuipn 09-05-2012 417 97   Download