ELISA kits

Part 2 book "The ELISA guidebook" includes content: Validation of diagnostic tests for infectious diseases, charting methods for internal quality control for competition ELISA, charting methods for internal quality control of indirect ELISA, ruggedness and robustness of tests - aspects of kit use and validation, more advanced statistical methods for quality assurance, test validation, and interpretation, internal quality control and external quality management of data in practice, immunochemical techniques.

273p oursky05 20-09-2023 1 1   Download

The aim of this study was to evaluate different immunochemical methods (Dot Blot, Immnoblotting and two different ELISA kits) for the detection of milk proteins in eleven raw and cooked model systems of meat products with 0 – 5000 ppm of powder deffated milk (PDM) and in nine raw and cooked model systems of meat products with 0-2000 ppm of dry whey (DW) and in eleven commercial meat products.

7p viisac 23-09-2023 3 1   Download

The aim of this study was to evaluate different ELISA kits for the detection of soy proteins in raw and cooked model systems (MS) added with soy protein concentrate 63% protein (SPC), and in commercial meat products. Nine bovine meat MS with 0-2000 ppm SPC, nine boneless ham cooked MS with 0-2000 ppm SPC and eight commercial meat products were analyzed.

5p viisac 23-09-2023 2 1   Download

Detection of milk traces was evaluated in cookies and extruded products model systems (MS) and in commercial products declaring milk presence through either the statement: “Contains…” or precautionary phrases. The aim of the study was to evaluate the performance of two different commercial ELISA kits for this purpose.

5p viisac 23-09-2023 5 1   Download

Ở Việt Nam, nấm Đông trùng hạ thảo (Cordyceps militaris) là dược liệu quý có nhiều ứng dụng trong phòng, trị bệnh và nâng cao sức khỏe con người. Phương pháp: Nghiên cứu tiến hành trên mô hình chuột nhắt trắng giảm năng sinh dục, xác định hàm lượng testosteron và 17β-estradiol bằng kỹ thuật ELISA theo quy trình của bộ kit được cung cấp bởi hãng Abcam.

6p viginnirometty 28-04-2022 28 1   Download

Immunization of pigs with an inactivated Mycoplasma hyopneumoniae vaccine (bacterin) generates hyperimmune serum that contains high concentrations of anti-M. hyopneumoniae IgG. Commercially available IgGELISA kits cannot distinguish between anti-M. hyopneumoniae IgG in inactivated bacterin-induced hyperimmune sera and convalescent sera resulting from natural M. hyopneumoniae infection.

10p vidarwin 23-02-2022 8 1   Download

This study aimed to screen the sera of goats and sheep from flocks suspected of Mycobacterium avium subsp. paratuberculosis (MAP) infection by a newly standardized Mce-truncated ELISA (Mt-ELISA) kit for the detection of antibodies against MAP. Four diagnostic applied tests were evaluated including Indigenous plate-ELISA (IP-ELISA), Mt-ELISA, fecal Polymerase Chain Reaction (f-PCR) and fecal culture (FC).

9p vidarwin 22-02-2022 9 1   Download

Nghiên cứu được thực hiện nhằm khảo sát đáp ứng miễn dịch của chó sau tiêm phòng vaccine dại. Mẫu huyết thanh được thu thập từ 40 chó ở huyện Phú Tân, tỉnh An Giang. Những chó này đã được tiêm vaccine phòng bệnh dại sau thời gian 9 tháng và 12 tháng. Mẫu huyết thanh sẽ được kiểm tra bằng phương pháp ELISA với bộ kít SERELISA® Rabies Ab Mono Indirect từ hãng Synbiotic của Pháp.

5p mudbound 10-12-2021 19 1   Download

The abuse of antibiotics leads to antibiotic resistance which may seriously threaten human and animal welfare, and growing levels of antibiotics or antibiotic-resistant bacteria in the environment increase the numbers of drug-resistant infection outbreaks. Therefore, many detection methods have been being developed to quickly assess antibiotic content and its residues in foods.

10p spiritedaway36 25-11-2021 7 0   Download

In this study, we developed a mAb against CA125 to be used as a capture antibody in ELISA technique for detecting CA125 concentration in sera of OC and BC patients. The results of CA125 level in this study were compared with that performed using a commercial CA125 ELISA kit.

8p nguaconbaynhay10 20-02-2021 9 2   Download

The present work was conducted to study the seroprevalence of Brucella infection amongst the cattle population in and around Rewa district (Madhya Pradesh). Seroprevalence study was conducted using Rose Bengal Plate Test and SVANOVIR Brucella Antibody c-ELISA kit. C-ELISA is OIE referred gold standard test for detecting Brucella antibodies.

5p cothumenhmong9 04-01-2021 8 1   Download

Trong nghiên cứu này, nhóm tác giả trình bày kết quả tách dòng và biểu hiện đoạn gen mã hóa vùng quyết định kháng nguyên 56 kDa của O. tsutsugamushi thuộc nhóm Karp từ mẫu HT-09 thu thập ở Bệnh viện Quân y 105 trong tế bào E. coli BL21. Kháng nguyên 56 kDa tái tổ hợp (HT-09) sau đó được tinh chế và sử dụng làm nguyên liệu để tạo bộ kit ELISA phát hiện O. tsutsugamushi.

8p viphilippine2711 30-12-2020 35 1   Download

This study was conducted to determine the seroprevalence of Hepatitis B virus infection and to determine the anti HBs antibody titres among the health care workers. A descriptive cross sectional study was conducted among 52 health care workers of a clinical laboratory. HBsAg detection and anti HBs titre were measured using commercially available ELISA and ELFA kits respectively.

6p gaocaolon9 22-12-2020 14 1   Download

In the present study, a total 210 blood/serum samples were collected from goats housed in various farms, c in Navsari and Valsad districts screened for Peste des petits ruminants (PPR) specific antibodies using PPR C-ELISA kit.

8p nguathienthan8 20-10-2020 10 2   Download

The single chain fragment antibody (scFv) antibodies were raised against CRY2B antigen by phage display technology. In antigen-antibody affinity selection the scFv antibody displaying phages concentration was increased (4.54 × 1010 to 2.3 ×1011) and fourth round phages were higher in concentration and affinity (1.06) to antigen revealed by ELISA.

11p nguaconbaynhay8 13-10-2020 10 0   Download

Serological Survey of measles virus among children 0 – 12 years of age was carried out in some States in North Western Nigeria. A total of 450 blood samples were collected and analyzed for measles virus IgM and IgG using commercial Elisa kit Obtained from Diagnostic Automation/ Cortez Diagnostics, California, the results was interpreted according to the manufacturer’s instruction.

11p nguaconbaynhay8 13-10-2020 23 3   Download

Nghiên cứu này làm rõ vai trò của nồng độ sMICB hòa tan trong huyết thanh ở bệnh nhân HCC liên quan đến HBV. Nghiên cứu được tiến hành trên 94 bệnh nhân ung thư biểu mô tế bào gan và nhóm chứng gồm 48 người khỏe mạnh. Protein sMICB trong huyết thanh được định lượng bằng bộ kit ELISA. Phương pháp phân tích kết quả được sử dụng phần mềm SPSS 18, Statview.

5p nanhankhuoctai7 01-07-2020 29 1   Download

Toxoplasmosis is a zoonosis caused by Toxoplasma gondii, an obligate intracellular parasite. It can cause significant morbidity and mortality in the developing fetus if the pregnant women get acute infection during pregnancy. The aim of the study was to assess the seroprevalence of Toxoplasmosis in antenatal women with bad obstetric history. The seropositivity was analysed according to age, bad obstetric history, socioeconomic and literacy status. A total of 160 antenatal women with BOH and 24 antenatal women with previous normal delivery as control group attending to Govt.

10p chauchaungayxua6 26-06-2020 9 0   Download

The objective of our study was to know the efficacy of the tests like Leptocheck WB IgM(ICT), PanBio IgM ELISA(ELISA), with MAT. Hundred and eleven serum samples from 54 patients with clinical suspicion of leptospirosis and 50 serum from asymptomatic subjects was tested for IgM antibodies by the kits. Out of 54 serum collected, 42.59% were positive by ICT, 48.14% by ELISA, and 53.7% by MAT.

6p nguaconbaynhay6 23-06-2020 10 0   Download

Dengue fever is increasing in incidence each year and with each monsoon season looms a fear of morbidity and mortality by dengue fever Measures by Government to prevent its spread have not been successful to a very large extent and probably require a more intensive approach towards the etiological causes.

5p nguathienthan5 04-06-2020 4 0   Download