Epidermal growth factor receptor

Luận án xác định sự thay đổi của HIP, EGFR ở mức độ mRNA và Protein tại mô ung thư vú so với u xơ tuyến vú lành; khảo sát sự thay đổi của HIP, EGFR ở mức độ mRNA và Protein trong các thể ung thư vú phân loại theo mô bệnh học.

39p hieuminhdo 03-09-2019 50 2   Download

Calmodulin (CaM) is phosphorylated in vitro and in vivo by multiple protein-serine/threonine and protein-tyrosine kinases. Casein kinase II and myosin light-chain kinase are two of the well established protein-serine/threonine kinases implicated in this process. On the other hand, within the protein-tyrosine kinases involved in the phosphorylation of CaM are receptors with tyrosine kinase activity, such as the insulin receptor and the epidermal growth factor receptor, and nonreceptor protein-tyrosine kinases, such as several members of the Src family kinases, Janus kinase 2, and p38Syk....

13p research12 01-06-2013 45 6   Download

A series of chimeric receptors was generated between the epidermal growth factor (EGF) receptor, ErbB-1, and its homologue, ErbB-4, to investigate the roles of the extracellular domains (I–IV) in the ligand specificities. As compared with ErbB-1 and the chimeras with both domains I and III of ErbB-1, the chimeras with only one of these domains exhibited reduced binding of 125I-labeled EGF. Particularly, the contribution of domain III was appreciably larger than that of domain I of ErbB-1 in 125I-labeled EGF binding.

7p research12 01-06-2013 48 5   Download

The hepatocyte growth factor-regulated tyrosine kinase substrate Hrs is an early endosomal protein that is thought to play a regulatory role in the trafficking of growth factor/ receptor complexes through early endosomes. Stimulation of cells with epidermal growth factor (EGF) rapidly leads to phosphorylation of Hrs, raising the question whether the receptor tyrosine kinase phosphorylates Hrs directly. Here, we present evidence that a downstream kinase, rather than the active receptor kinase is responsible. ...

7p research12 23-04-2013 36 2   Download

Nitric oxide (NO • ) strongly inhibits the proliferation of human A431 tumour cells. It also inhibits tyrosine phos-phorylation of a 170-kDa band corresponding to the epidermal growth factor receptor (EGFR) and induces the phosphorylation at tyrosine residue(s) of a 58-kDa protein which we have denoted NOIPP-58 (nitric oxide-induced 58-kDa phosphoprotein). The NO • -induced phosphoryla-tion of NOIPP-58 is strictly dependent on the presence of EGF. Phosphorylation of NOIPP-58 and inhibition of the phosphorylation of the band corresponding to EGFR are both cGMP-independent processes. ...

10p fptmusic 16-04-2013 29 3   Download

Gangliosideshavebeen found toreside inglycosphingolipid-enriched microdomains (GEM) of the plasma membrane and to be involved in the regulation of epidermal growth factor receptor (EGFr or ErbB1) activity.To gain further insight into the mechanisms involved in EGFr modulation by gangliosides, we investigated the distribution of EGFr family members in the plasma membrane of CHO-K1 cells, which were genetically modified to express different gan-glioside molecules or depleted of glycolipids.

10p dell39 03-04-2013 43 5   Download

Sponges (phylum Porifera) live in a symbiotic relationship with microorganisms, primarily bacteria. Until now, mole-cular proof for the capacity of sponges to recognize fungi in the surroundingaqueousmilieuhas not beenavailable.Here we demonstrate, for the demospongeSuberites domuncula (Porifera, Demospongiae, Hadromerida), a cell surface receptor that recognizes (1fi3)-b-D-glucans, e.g. curdlan or laminarin. This receptor, the (1fi3)-b-D-glucan-binding protein, was identified and its cDNA analysed.

14p dell39 03-04-2013 37 5   Download

Human airway trypsin-like protease (HAT), a serine protease found in the sputum of patients with chronic airway diseases, is an agonist of protease-activated receptor-2 (PAR-2). Previous results have shown that HAT enhances the release of amphiregulin (AR); further, it causes MUC5AC gene expression through the AR-epidermal growth factor receptor pathway in the airway epithelial cell line NCI-H292.

13p dell39 27-03-2013 34 1   Download

Two H7721 human hepatocarcinoma cell lines showing moderate and high expression of a1,3-fucosyltransferase (FucT)-VII cDNA were established and designated FucTVII-M and FucTVII-H, respectively. In a1,3-FucT-VII-transfected cells, expression of insulin receptor (InR)a- and bsubunits and epidermal growth factor receptor (EGFR) on the cell surface and in cells, as well as the sialyl Lewis X (SLe x , the product of a1,3-FucT-VII) content of the EGFR were unchanged.

13p inspiron33 23-03-2013 41 4   Download

O-Fucosylation is a post-translational glycosylation in which anO-fucose is covalently attached to the hydroxyl group of a specific serine or threon-ine residue. This modification occurs within the consensus sequence C2X4)5(S⁄T)C3 present on epidermal growth factor-like repeats of several proteins, including the Notch receptors and their ligands.

10p galaxyss3 21-03-2013 45 4   Download

Epidermal growth factor receptor (EGFR)-mediated signal transduction is often hyperactivated in tumour cells and therefore considered a promising target for cancer therapy. A number of computational models have been developed which describe the pathway in great detail.

13p media19 04-03-2013 39 3   Download

We report the development of a high-level bacterial expression system for the Alzheimer’s disease-associated amyloid b-peptide (Ab), together with a scaleable and inexpensive purification procedure. Ab(1–40) and Ab(1–42) coding sequences together with added ATG codons were cloned directly into a Pet vector to facilitate production of Met-Ab(1–40) and Met-Ab(1– 42), referred to as Ab(L1–40) and Ab(L1–42), respectively.

11p vinaphone15 27-02-2013 48 2   Download

Alterations resulting in enhanced epidermal growth factor receptor (EGFR) expression or function have been documented in a variety of tumors. Therefore, EGFR-tyrosine kinase is a promising therapeutic target. Althoughin vitro andin vivo studies have shown the anti-tumor activity of EGFR-tyrosine kinase inhibitors against various tumor types, little is known about the mechanism by which such inhibitors effect their anti-tumor action.

22p vinaphone15 27-02-2013 43 4   Download

Controlled activation of epidermal growth factor receptor (EGFR) is sys-tematically guaranteed at the molecular level; however, aberrant activation of EGFR is frequently found in cancer. Transcription induced by EGFR activation often involves the coordinated expression of genes that positively and negatively regulate the original signaling pathway; therefore, altera-tions in EGFR kinase activity may reflect changes in gene expression asso-ciated with the pathway.

13p vinaphone15 25-02-2013 34 5   Download

The function of S100A4, a member of the calcium-binding S100 protein family, has been associated with tumor invasion and metastasis. Although an essential pro-metastatic role of extracellular S100A4 in tumor progres-sion has been demonstrated, the identification of the precise underlying mechanisms and protein partners (receptors) has remained elusive.

13p viettel02 20-02-2013 38 2   Download

Both the epidermal growth factor receptor signaling pathway and micro-RNA (miRNA) play an important role in lung cancer development and progression. To address the potential role of miRNA in epidermal growth factor receptor signaling, we identified miR-125a-5p as a downstream target, using an miRNA array.

8p viettel02 20-02-2013 41 3   Download

We previously reported the use of a humanized bi-specific diabody that targets epidermal growth factor receptor and CD3 (hEx3-Db) for cancer immunotherapy. Bacterial expression can be used to express small recombi-nant antibodies on a large scale; however, their overexpression often results in the formation of insoluble aggregates, and in most cases artificial affinity peptide tags need to be fused to the antibodies for purification by affinity chromatography.

11p mobifone23 18-01-2013 42 3   Download

A common physiological response of organisms to environmental stresses is the increase in expression of heat shock proteins (Hsps). In insects, this process has been widely examined for heat stress, but the response to cold stress has been far less studied.

16p mobifone23 18-01-2013 30 4   Download

In this review, we first describe the mechanisms by which the epidermal growth factor receptor generates a Ca 2+ signal and, subsequently, we com-pile the available experimental evidence regarding the role that the Ca 2+ ⁄calmodulin complex, formed after the rise in cytosolic free Ca 2+ concentration, exerts on the receptor.

11p mobifone23 18-01-2013 32 5   Download

The function of the epidermal growth factor receptor (EGFR) is dysregu-lated in various types of malignancy as a result of gene amplification, mutations, or abnormally increased ligand production. Therefore, the tyro-sine kinase activity of the EGFR is a promising therapeutic target.

11p mobifone23 18-01-2013 43 3   Download