Eras of medicine

Era is a highly conserved GTPase essential for bacterial growth. The N-terminal part of Era contains a conserved GTPase domain, whereas the C-terminal part of the protein contains anRNA- andmembrane-binding domain, theKH domain. To investigate whether the binding of Era to 16S rRNA and membrane requires its GTPase activity and whether the GTPase domain is essential for these acti-vities, the N- and C-terminal parts of the Streptococ-cus pneumoniaeEra –Era-N(aminoacids 1–185) andEra-C (amino acids 141–299), respectively – were expressed and purified. ...

9p tumor12 20-04-2013 32 3   Download

The availability of extensive genomic information and content has spawned an era of high-throughput screening that is generating large sets of func-tional genomic data. In particular, the need to understand the biochemical wiring within a cell has introduced novel approaches to map the intricate networks of biological interactions arising from the interactions of proteins.

14p fptmusic 12-04-2013 29 2   Download

We have defined thein vivo andin vitro metabolic fate of internalized chol-era toxin (CT) in the endosomal apparatus of rat liver. In vivo, CT was internalized and accumulated in endosomes where it underwent degrada-tion in a pH-dependent manner. In vitro proteolysis of CT using an endo-somal lysate required an acidic pH and was sensitive to pepstatin A, an inhibitor of aspartic acid proteases.

13p fptmusic 11-04-2013 35 3   Download

Research in diatom biology has entered the postgenomic era since the recent completion of the Thalassiosira pseudonanagenome project. How-ever, the molecular tools available for genetic manipulation of diatoms are still sparse, impeding the functional analysis of diatom genes in vivo. Here we describe the first method for inducible gene expression in transgenic diatoms.

11p fptmusic 11-04-2013 45 2   Download

In the postgenomic era it is essential that protein sequences are annotated correctly in order to help in the assignment of their putative functions. Over 1300 proteins in current pro-tein sequence databases are predicted to contain a PAS domainbaseduponaminoacid sequence alignments.Oneof theproblemswith the current annotationof thePASdomain is that this domain exhibits limited similarity at the amino acid sequence level.

11p dell39 03-04-2013 37 5   Download

Estrogen is known to influence glucose homeostasis but the role of estrogen receptors in muscle glucose metabolism is unknown. Therefore, we investigated the expression of the two estrogen receptors, ERa and ERb and their influence on regulation of GLUT4, and its associated structural protein, caveolin-1, in mouse muscle. ERaand ERbare co-expressed in the nuclei of most muscle cells and their levels were not affected by absence of estradiol (in aromatase knockout, ArKO, mice).

6p inspiron33 26-03-2013 46 4   Download