Expression rates

Assessed the level of mRNA expression of CIZ1b, VEGF, EGFR mutations and the rate of Merkel cell infection in patients with non-small cell lung cancer. Assessed the level of mRNA expression of CIZ1b, VEGF, EGFR mutations and the rate of Merkel cell infection in patients with non-small cell lung cancer.

32p trinhthamhodang7 31-08-2020 35 3   Download

Evaluation of response rate and side effects of pre-operative and/or pre-radiotherapy neo-adjuvant TC chemotherapy regimen for treatment of tongue cancer at stages III and IV (M0). Determination of expression rates of p53, EGFR and Her2 markers and some factors related to overall survival of patient with stages III and IV tongue cancer.

25p angicungduoc6 21-07-2020 27 3   Download

The importance of two putative Zn2+-binding (Asp347, Glu429) and two catalytic (Arg431, Lys354) residues in the tomato leucine aminopeptidase (LAP-A) function was tested. The impact of substitutions at these positions, corresponding to the bovine LAP residues Asp255, Glu334, Arg336, and Lys262, was evaluated in His6–LAP-A fusion proteins expressed in Escherichia coli. Sixty-five percent of the mutant His6–LAP-A proteins were unstable or had complete or partial defects in hexamer assembly or stability. The activity of hexameric His6–LAP-As on Xaa-Leu and Leu-Xaa dipeptides was tested.

11p system191 01-06-2013 44 4   Download

Cloning and over-expression of human glucose 6-phosphate dehydrogenase (Glc6P dehydrogenase) has for the first time allowed a detailed kinetic study of a preparation that is genetically homogeneous and in which all the protein molecules are of identical age. The steady-state kinetics of the recombinant enzyme, studied by fluorimetric initial-rate measurements, gave converging linear Lineweaver–Burk plots as expected for a ternary-complex mechanism.

8p research12 01-06-2013 66 4   Download

Two genes (CM-AAT1 and CM-AAT2) with strong sequence homology (87% identity at the protein level) putatively involved in the formation of aroma volatile esters have been isolated from Charentais melon fruit. They belong to a large and highly divergent family of multifunctional plant acyl-transferases and show at most 21% identity to the only other fruit acyl-transferase characterized so far in strawberry. RT-PCR studies indicated that both genes were specifically expressed in fruit at increasing rates in the early and mid phases of ripening.

8p research12 01-06-2013 53 4   Download

Thermobifida fusca xyloglucan-specific endo-b-1,4-gluca-nase (Xeg)74 and the Xeg74 catalytic domain (CD) were cloned, expressed inEscherichia coli, purified and charac-terized.This enzymehas aglycohydrolase family-74CDthat is a specific xyloglucanase followed by a family-2 carbo-hydrate binding module at the C terminus.The Michaelis constant (Km) andmaximal rate (Vmax) values for hydrolysis of tamarind seed xyloglucan (tamXG) are 2.4lMand 966lmol xyloglucan oligosaccharides (XGOs) min )1 Ælmol protein )1 ....

9p tumor12 20-04-2013 49 5   Download

Heterologous expression of the putative open reading frame MJ0303 ofMethanococcus jannaschiiprovided a recombin-ant protein catalysing the formation of the riboflavin precursor, 6,7-dimethyl-8-ribityllumazine, by condensation of 5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione and 3,4-dihydroxy-2-butanone 4-phosphate. Steady state kinetic analysis at 37
C and pH 7.0 indicated a catalytic rate of 11 nmolÆmg )1 Æmin )1 ;Kmvalues for 5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione and 3,4-dihydroxybutanone 4-phosphate were 12.5 and 52lM, respectively....

8p tumor12 20-04-2013 37 3   Download

Human novel reductase 1 (NR1) is an NADPH dependent diflavin oxidoreductase related to cytochrome P450reduc-tase (CPR). The FAD/NADPH- and FMN-binding domains of NR1 have been expressed and purified and their redox properties studied by stopped-flow and steady-state kinetic methods, and by potentiometry.

12p tumor12 20-04-2013 42 2   Download

Plasminogen activator inhibitor type-2 (PAI-2) is a nonconventional serine protease inhibitor (serpin) with unique and tantalizing properties that is generally considered to be an authentic and physiological inhibitor of uro-kinase. However, the fact that only a small percentage of PAI-2 is secreted has been a long-standing argument for alternative roles for this serpin.

10p fptmusic 12-04-2013 41 2   Download

The addition of glucose toSaccharomyces cerevisiaecells causes reprogramming of gene expression. Glucose is sensed bymembrane receptorsaswell as (so far elusive) intracellular sensing mechanisms. The availability of four yeast strains that display different hexose uptake capacities allowed us to study glucose-induced effects at different glycolytic rates. Rapid glucose responses were observed in all strains able to take up glucose, consistent with intracellular sensing.

10p awards 05-04-2013 34 4   Download

Glutamine is an essential amino acid in cancer cells and is required for the growth of many other cell types. Glutami-nase activity is positively correlated with malignancy in tumours andwith growth rate in normal cells. In the present work, Ehrlich ascites tumour cells, and their derivative, 0.28AS-2 cells, expressing antisense glutaminase mRNA, were assayed for apoptosis induced by methotrexate and hydrogen peroxide. It is shown that Ehrlich ascites tumour cells, expressing antisense mRNA for glutaminase, contain lower levels of glutathione than normal ascites cells. In addition, 0.

9p awards 05-04-2013 47 4   Download

An open reading frame (Acc. no. P50740) on theBacillus subtilis chromosome extending from bp 184 997–186 043 with similarity to theidi-2 gene ofStreptomycessp. CL190 specifying type II isopentenyl diphosphate isomerase was expressed in a recombinant Escherichia colistrain. The recombinant protein with a subunit mass of 39 kDa was purified to apparent homogeneity by column chromatog-raphy. The protein was shown to catalyse the conversion of dimethylallyl diphosphate into isopentenyl diphosphate and vice versa at rates of 0.23 and 0.

12p dell39 03-04-2013 45 4   Download

ThepyrGgene fromLactococcus lactisencodes CTP syn-thase (EC 6.4.3.2), an enzyme converting UTP to CTP. A series of strains were constructed with different levels of pyrGexpression by insertion of synthetic constitutive pro-moters with different strengths in front ofpyrG.These strains expressed pyrGlevels in a range from 3 to 665% relative to thewild-type expression level.Decreasing the level of CTP synthase to 43% had no effect on the growth rate, showing that the capacity of CTP synthase in the cell is in excess in a wild-type strain. ...

8p dell39 03-04-2013 46 5   Download

We cloned a cDNA for aDrosophila melanogasterhomo-logue ofmammalian heme oxygenase (HO) and constructed a bacterial expression system of a truncated, soluble form ofD. melanogasterHO (DmDHO). The purified DmDHO degraded hemin to biliverdin, CO and iron in the presence of reducing systems such as NADPH/cytochrome P450 reductase and sodium ascorbate, although the reaction rate was slower than that of mammalian HOs. Some properties of DmHO, however, are quite different from other known HOs.

12p dell39 03-04-2013 49 4   Download

GTP cyclohydrolase I (GCH) is the rate-limiting enzyme for the synthesis of tetrahydrobiopterin and its activity is important in the regulation of monoamine neurotransmit-ters such as dopamine, norepinephrine and serotonin. We have studied the action of divalent cations on the enzyme activity of purified recombinant human GCH expressed in Escherichia coli. First, we showed that the enzyme activity is dependent on the concentration ofMg-freeGTP.

7p dell39 03-04-2013 34 6   Download

Parvalbumin (PV), a small cytosolic protein belonging to the family of EF-hand calcium-binding proteins, is highly expressed in mammalian fast-twitch muscle fibers. By acting as a ‘slow-onset’ Ca 2+ buffer, PV does not affect the rapid contraction phase, but significantly contributes to increase the rate of relaxation, as demonstrated in PV–⁄– mice.

13p dell39 27-03-2013 42 3   Download

TheispC gene of Arabidopsis thalianawas expressed in pseudomature form without the putative plastid-targeting sequence in a recombinant Escherichia colistrain. The recombinant protein was purified by affinity chromatography and was shown to catalyze the formation of 2C-methyl-d-erythritol 4-phosphate from 1-deoxy-d-xylulose 5-phosphate at a rate of 5.6lmolÆmin )1 Æmg )1 (kcat 4.4 s )1 ). The Michaelis constants for 1-deoxy-d-xylulose 5-phosphate and the cosubstrate NADPH are 132 and 30lm, respectively. ...

13p inspiron33 25-03-2013 38 5   Download

Loss of E-cadherin-mediated cell–cell adhesion and expression of proteolytic enzymes characterize the transition from benign lesions to invasive, metastatic tumor, a rate-limiting step in the progression from adenoma to carcinoma in vivo. A soluble E-cadherin fragment found recently in the serum and urine of cancer patients has been shown to disrupt cell–cell adhe-sion and to drive cell invasion in a dominant-interfering manner.

14p inspiron33 23-03-2013 42 3   Download

Transcription from HIV-1 proviral DNA is a rate-determining step for HIV-1 replication. Interaction between the cyclin T1 (CycT1) subunit of positive transcription elongation factor b (P-TEFb) and the Tat transacti-vator protein of HIV-1 is crucial for viral transcription.

10p vinaphone15 25-02-2013 31 3   Download

p63 is a member of the p53 tumour suppressor family that includes p73. Thep63gene encodes a protein comprising an N-terminal transactivation domain, a DNA binding domain and an oligomerization domain, but var-ies in the organization of the C-terminus as a result of complex alternative splicing.

14p cosis54 05-01-2013 35 5   Download