Báo cáo khoa học: Isoprenoid biosynthesis in plants – 2C-methyl-D-erythritol4-phosphate synthase (IspC protein) of Arabidopsis thaliana
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TheispC gene of Arabidopsis thalianawas expressed in pseudomature form without the putative plastid-targeting sequence in a recombinant Escherichia colistrain. The recombinant protein was purified by affinity chromatography and was shown to catalyze the formation of 2C-methyl-d-erythritol 4-phosphate from 1-deoxy-d-xylulose 5-phosphate at a rate of 5.6lmolÆmin )1 Æmg )1 (kcat 4.4 s )1 ). The Michaelis constants for 1-deoxy-d-xylulose 5-phosphate and the cosubstrate NADPH are 132 and 30lm, respectively. ...
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