Fibroblast

A large number of studies have demonstrated the wound-healing effects of LLLT in vitro, in animal models, and in clinical practice. However, there are also differences in the study results, which are dose and wavelength dependent of LLLT. Evaluation of the wound healing process in experimental animals treated with low- level laser therapy in clinical and histopathology.

8p viengfa 23-10-2024 3 0   Download

This study investigated the in vitro effects of ZnO NP and chemical UV filters commonly used sunscreens on the function of immortalised and primary skin cells. ZnO particulates (30 nm, 80 nm and 200 nm) were compared to the chemical UV filters: butylmethoxydibenzoylmethane (BMDBM) ethylhexylmethoxycinnamate (EHMC), ethylhexylsalicylate (EHS), homomenthylsalicylate (HMS) and 4-methylbenzylidenecamphor (MBC). Human epidermal keratinocytes (NHEK), epidermal melanocytes (NHEM), dermal fibroblasts (NHDF) and the immortalised keratinocyte HaCaT cell line were used in this study.

324p runthenight05 01-03-2023 9 3   Download

Sản phẩm vật liệu (dạng gel) nanocellulose kết hợp với chiết xuất nhung hươu do Trung tâm nghiên cứu triển khai khu công nghệ cao thành phố Hồ Chí Minh phối hợp với công ty TNHH Geneworld đạt tiêu chuẩn cơ sở, được nghiên cứu độc tính trên Fibroblast, đánh giá khả năng ngừa phát triển vi khuẩn, nghiên cứu độc tính cấp và bán trường diễn. Để có đầy đủ cơ sở tiến hành trên lâm sàng, tác giả tiến hành nghiên cứu nhằm mục tiêu đánh giá khả năng dung nạp trên da lành của động vật thực nghiệm và ảnh hưởng của chế phế khi dùng lâu dài trên động vật.

9p runthenight01 18-11-2022 12 2   Download

Bromelain trong dứa và curcumin từ nghệ kết hợp để tạo thành một hiệu ứng chống viêm, tăng tốc lành vết thương. Áp dụng công nghệ nano để chuẩn bị phức hợp silica-curcumin-bromelain tạo ra phức hợp hạt nano giúp tăng cường khả năng của cơ thể được tiếp xúc và hấp thu bởi cơ thể trong khi duy trì hoạt động sinh học.

5p viclerkmaxwel 16-02-2022 31 3   Download

Fibroblast growth factor-2 (FGF-2) is a multifunctional protein that plays an important role in the regulation of proliferation, differentiation and migration of a variety of cells. The recombinant human FGF-2 (rhFGF-2) is currently used in stem cell culture, medicine and cosmetic products. In this study, we aim to produce secreted rhFGF-2 protein from a Pichia pastoris strain containing multiple copies of the fgf-2 gene to eliminate the disadvantages of intracellular expression systems

9p larachdumlanat127 02-01-2021 13 2   Download

Nghiên cứu này được thực hiện với mục đích khảo sát một số đặc tính của màng BC do A. xylinum tạo ra và khả năng sử dụng của màng BC làm giá đỡ nuôi cấy tế bào fibroblast thu được từ chuột nhắt trắng. Đây là nghiên cứu bước đầu cho các nghiên cứu tiếp theo sử dụng vật liệu BC làm giá đỡ trong kỹ nghệ mô.

7p jangni 16-04-2018 109 3   Download

Luận văn cao học nhằm thực hiện một số nhiệm vụ cơ bản sau: Xác định độc tính của chất lỏng từ lên một số dòng tế bào ung thư và Fibroblast; khảo sát khả năng tạo tương phản ảnh của hạt nano từ bằng phương pháp chụp cộng hưởng từ hạt nhân (MRI); thử liệu pháp nhiệt trị trên mô hình ung thư thực nghiệm.

85p change14 07-07-2016 101 10   Download

Đề tài: Phân lập và tạo dòng nguyên bào sợi (fibroblast) người từ bánh nhau nhằm xây dựng một phương pháp nuôi cấy tối ưu đối với nguyên bào sợi từ mô gai nhau, đánh giá hiệu quả của các phương pháp nuôi cấy khác nhau đối với dòng nguyên bào sợi lấy từ mẫu mô gai nhau, so sánh khả năng tăng trưởng của nguyên bào sợi trên các loại môi trường nuôi khác nhau,...Mời bạn đọc cùng tham khảo.

82p langtuthangpro 16-06-2014 195 20   Download

Viral infection of fibroblastic and endothelial cells leads to the transient synthesis of interferon-b(IFN-b). The down-regulation of IFN-bsynthesis after infection results both from transcriptional repression of the IFN-bgene and rapid degradation of mRNA. As with many cytokine mRNAs, IFN-bmRNA contains an AU-rich element (ARE) in its 3¢-untranslated region (UTR). AREs are known tomediate mRNAdeadenylation and destabilization. Depending on the class of ARE, deadenylation was shown to occur through synchronous or asynchronous mechanisms....

8p tumor12 20-04-2013 23 2   Download

Functional cooperationbetween integrins andgrowth factor receptors has been reported for several systems, oneofwhich is the modulation of insulin signaling by avb3 integrin. Plasminogen activator inhibitor type-1 (PAI-1), competes withavb3 integrin for vitronectin (VN) binding. Here we report that PAI-1, in a VN-dependent manner, prevents the cooperation of avb3 integrin with insulin signaling in NIH3T3 fibroblasts, resulting in a decrease in insulin-induced protein kinase B (PKB) phosphorylation, vascular endothelial growth factor (VEGF) expression and cell migration. ...

8p tumor12 20-04-2013 35 3   Download

Minichromosome maintenance proteins (Mcm) 2–7 play essential roles in eukaryotic DNA replication. Several reports have indicated the usefulness of Mcm proteins as markers of cancer cells in histopathological diagnosis. However, their mode of expression and pathophysiological significance in cancer cells remain to be clarified. We com-paredthe level of expressionofMcmproteins amonghuman HeLa uterine cervical carcinoma cells, SV40-transformed human fibroblast GM00637 cells andnormal human fibro-blastWI-38 cells....

13p tumor12 20-04-2013 30 2   Download

The expression of the major protein kinase C substrate MARCKS (myristoylatedalanine-richCkinase substrate) is controlled by the stability of its mRNA. While the MARCKS mRNA is long living in quiescent fibroblasts (t1/2¼14 h), its half-life time is drastically reduced (t1/2¼2 h) in cells treated with phorbol esters to activate protein kinase C (PKC) or treatedwith growth factors. In a first step to study the underlying mechanism we identified both acis-element on the MARCKS mRNA and the cor-responding trans-acting factors. ...

16p tumor12 20-04-2013 39 3   Download

TheST2gene, which is specifically induced by growth sti-mulation in fibroblasts, encodes interleukin-1 receptor-rela-tedproteins and iswidely expressed in hematopoietic, helper T, and various cancer cells. However, the physiological as well as pathological functions of the ST2 gene products are not yet fully understood. In this study, we analyzed the expression of theST2gene in human glioma cell lines and human brain tumor samples with real-time polymerase chain reactionmethod,

8p tumor12 20-04-2013 48 4   Download

HR12 is anovel farnesyltransferase inhibitor (FTI).Wehave shown previously that HR12 induces phenotypic reversion of H-ras V12 -transformed Rat1 (Rat1/ras) fibroblasts. This reversion was characterized by formation of cell–cell con-tacts, focal adhesions and stress fibers. Here we show that HR12 inhibits anchorage independent and dependent growth of Rat1/ras cells. HR12 also suppresses motility and proliferation of Rat1/ras cells, in a wound healing assay. Rat1 fibroblasts transformed with myristoylated H-ras V12 (Rat1/myr-ras) were resistant to HR12....

14p fptmusic 16-04-2013 38 2   Download

Cyclins are the essential regulatory subunits of cyclin-dependent protein kinases. They accumulate and disappear periodically at specific phases of the cell cycle. Here we investigated whether variations in cyclin mRNA levels in exponentially growing cells can be attributed to changes in mRNA stability. Mouse EL4 lymphoma cells and 3T3 fibroblasts were synchron-ized by elutriation or cell sorting.

13p fptmusic 12-04-2013 57 3   Download

Growth factors, including fibroblast growth factor-2 (FGF-2) and transform-ing growth factor-b(TGF-b) regulate fibroblast function, differentiation and proliferation. S100A8 and S100A9 are members of the S100 family of Ca 2+ -binding proteins and are now accepted as markers of inflammation. They are expressed by keratinocytes and inflammatory cells in human⁄murine wounds and by appropriately activated macrophages, endothelial cells, epithelial cells and keratinocytesin vitro.

17p awards 06-04-2013 33 2   Download

SHIP2, the ubiquitous SH2 domain containing inositol 5-phosphatase, includes a series of protein interacting domains and has the ability to dephosphorylate phosphatidylinositol 3,4,5-trisphosphate [PtdIns(3,4,5)P3 ] in vitro. The present study, which was undertaken to evaluate the impact of SHIP2 on PtdIns(3,4,5)P3 levels, was performed in a mouse embryonic fibroblast (MEF) model using SHIP2 deficient (–⁄–) MEF cells derived from knockout mice.

0p awards 06-04-2013 40 1   Download

Although group IIA secretory phospholipase A2 (sPLA2-IIA) is known to be abundantly present in the joints of patients with rheumatoid arthritis (RA), expression of other sPLA2 s in this disease has remained unknown. In this study, we examined the expression and localization of six sPLA2s (groups IIA, IID, IIE, IIF, V and X) in human RA.

18p awards 05-04-2013 40 4   Download

Signal transduction pathways are often embedded in com-plex networks, which result from interactions between pathways and feedback circuitry. In order to understand such networks, qualitative information on which inter-actions take place and quantitative data on their strength become essential.

9p awards 05-04-2013 45 5   Download

Several oncogenic proteins are known to influence cellular glycosylation. In particular, transfection of codon 12 point mutated H-Ras increases CMP-Neu5Ac: Galb1,4GlcNAc a2,6-sialyltransferase I (ST6Gal I) activity in rodent fibroblasts. Given that Ras mediates its effects through at least three secondary effector pathways (Raf, RalGEFs and PI3K) and that transcriptional control ofmouse ST6Gal I is achieved by the selective use of multiple promoters, we attempted to identifywhichof these parameters are involved in linking the Ras signal to ST6Gal I gene transcription in mouse fibroblasts. ...

12p awards 05-04-2013 42 3   Download