Gene regulation

The C2H2 zinc finger protein ZAT12 has been classified as a plant core abiotic stress response gene in the early response to multiple stresses. ZAT12 links the iron deficiency and oxidative stress responses through the direct interaction with/and negative regulation of a central regulator - FIT.

8p vibecca 01-10-2024 3 1   Download

Studies on nanoparticle-protein interaction have also not been studied. This would give information on ratio influence on the structural alteration of EGCg in EGCg-AuNPs and its functionality in vivo. Herein, the optimisation of EGCg-AuNPs will be determined with a large pool of stoichiometric synthesises of gold and EGCg. These optimised EGCg-AuNPs would then be studied with bovine serum albumin (BSA) for protein interactions, binding and thermodynamics.

177p runthenight07 01-03-2023 8 3   Download

Recent sequencing technologies enable joint quantification of promoters and their enhancer regions, allowing inference of enhancer–promoter links. We show that current enhancer–promoter inference methods produce a high rate of false positive links. We introduce FOCS, a new inference method, and by benchmarking against ChIAPET, HiChIP, and eQTL data show that it results in lower false discovery rates and at the same time higher inference power.

14p vimichaelfaraday 25-03-2022 16 2   Download

Transcripts with alternative 5′-untranslated regions (UTRs) result from the activity of alternative promoters and they can determine gene expression by influencing its stability and translational efficiency, thus executing complex regulation of developmental, physiological and pathological processes.

9p vielonmusk 21-01-2022 11 0   Download

RNA polymerase II (Pol II) is a 12 subunit protein complex from yeast to human that is required for gene expression. Gdown1 containing Pol II [Pol II (G)] is a special form of Pol II that is catalytically inactive and heavily depends on the 30-subunit Mediator complex for its activator and basal dependent function in vitro. Here we report for the first time, the identification and the generation of a 15-subunit human Mediator complex via the novel multibac baculovirus expression system that is fully responsive to Pol II (G).

9p thiencuuchu 27-11-2021 21 3   Download

Drosophila model is intensively studied for the development of cancer. The diminutive (dMyc), a homolog of the human MYC gene, is responsible for cell- apoptosis and its upregulation is responsible for determining the fate of cancerous growth in humans and Drosophila model. This work implores the requirement of dMyc and its expression as one of the major regulator of cancer with other proteins and repression of dMyc mRNA in Drosophila S2 cells.

7p thiencuuchu 27-11-2021 15 1   Download

Heterogeneous nuclear ribonucleoprotein (HNRNP) A1 and A2 are the most abundant HNRNPs with nearly identical functions, and play important roles in regulating gene expression at multiple levels (i.e. transcription, posttranscription, and translation). However, the expression and regulation mechanism of HNRNPA1 and A2 themselves remain unclear. In this study, the amino acid sequences of HNRNPA1 and HNRNPA2 were compared and found to have 78% and 86% homology in key functional domains.

10p thiencuuchu 27-11-2021 10 1   Download

The objectives of the thesis: Investigate the role of A20 in regulating four physiological functions of DCs including maturation, cytokine secretion, migration and apoptosis; investigate the effect of A20 on the regulating three molecular signals including NF-κB, STAT1 and STAT3 related to the physiological process of DCs.

27p capheviahe27 23-02-2021 14 4   Download

Fibroblast growth factor-2 (FGF-2) is a multifunctional protein that plays an important role in the regulation of proliferation, differentiation and migration of a variety of cells. The recombinant human FGF-2 (rhFGF-2) is currently used in stem cell culture, medicine and cosmetic products. In this study, we aim to produce secreted rhFGF-2 protein from a Pichia pastoris strain containing multiple copies of the fgf-2 gene to eliminate the disadvantages of intracellular expression systems

9p larachdumlanat127 02-01-2021 13 2   Download

Bài viết tiến hành xác định gene BPSS1498 mã hóa hemolysin co-regulated protein 1 (hcp1), tạo dòng và biểu hiện protein bằng tế bào E. coli BL21(DE3) pLysS. Protein hcp1 có kích thước 18,7 kDa được tinh sạch. Western blot chứng minh hcp1 gắn kết đặc hiệu với kháng thể IgG của bệnh nhân nhiễm melioidosis.

6p kequaidan8 03-11-2020 50 3   Download

The development of fruits without fertilization is known as parthenocarpy. Parthenocarpy improves the fruit quality, processing attributes, production and productivity of vegetable crops like tomato, cucumber, watermelon etc. Absence of seeds can enhances the shelf life of the fruits, allowing a better conservation, fruit set in adverse climatic conditions, early and offseason production of vegetable crops. Therefore, it is important to ensure yield stability regardless of environmental conditions.

20p gaocaolon5 14-06-2020 16 3   Download

Nuclear factor erythroid-2 related factor 2 (Nrf2) is a crucial transcription factor that regulates the expression of defensive antioxidants and detoxification enzymes in cells. In a previous study, we showed that expression of the Nrf2 gene is regulated by an epigenetic modification. Rauvolfia verticillata, a traditional Chinese herbal medicine widely used in China, possesses anticancer and antioxidant effects. In this study, we investigated how Nrf2 is epigenetically regulated by reserpine, the main active component in R. verticillata, in mouse skin epidermal JB6 P+ cells.

11p caothientrangnguyen 09-05-2020 16 2   Download

The basic helix-loop-helix transcription factors play important roles in diverse cellular and molecular processes. Comparative functional genomics can provide powerful approaches to draw inferences about gene function and evolution among species.

21p vishikamaru2711 29-04-2020 15 3   Download

The auxin response factor (ARF) gene family is involved in plant development and hormone regulation. Although the ARF gene family has been studied in some plant species, its structural features, molecular evolution, and expression profiling in Brachypodium distachyon L. are still not clear.

15p vishikamaru2711 29-04-2020 31 2   Download

Increasing the productivity of rapeseed as one of the widely cultivated oil crops in the world is of upmost importance. As flowering time and plant architecture play a key role in the regulation of rapeseed yield, understanding the genetic mechanism underlying these traits can boost the rapeseed breeding

12p vishikamaru2711 29-04-2020 16 1   Download

Breast cancer is the most common cancer among women and the molecular pathways that play main roles in breast cancer regulation are still not completely understood. MicroRNAs (miRNAs) and transcription factors (TFs) are important regulators of gene expression.

17p nutifooddau 27-01-2019 13 2   Download

MicroRNAs (miRNAs) are small noncoding RNAs of about 19–25 nt that regulate gene expression posttranscriptionally under various cellular conditions, including apoptosis. The miRNAs involved in modulation of apoptotic events in T cells are partially known.

10p nutifooddau 27-01-2019 23 2   Download

Breast cancer (BC), the most common type of cancer among women worldwide, is a polygenetic disease which is caused by the interaction of several genes. Understanding the genetic factors for early diagnosis of BC is crucial to ensure the survival of BC patients. MicroRNA 27a (miR-27a), an oncogenic miRNA, has been predicted to target on the tumor suppressor ZBTB10 that can regulate many processes of cell.

11p bautroibinhyen17 13-02-2017 49 2   Download

In chapter 7 we will explore one strategy bacteria employ to control the expression of their genes: by grouping functionally related genes together so they can be regulated together easily. Such a group of contiguous, coordinately controlled genes is called an operon.

49p tangtuy05 30-03-2016 56 2   Download

A novel vector platform for vitamin H-inducible transgene expression in mammalian cells have therefore designed a strategy to convert antibiotic-responsive transcription factors into gene regulation systems responsive to non-toxic biotin, also known as vitamin H.

9p thuyancn 01-06-2015 43 5   Download