Immune response

General objectives: Creating of attenuated mutant Vibrio parahaemolyticus strains causing immune response for fish to develop production of vaccines against nephrotic and hepatic necrosis disease for some marine fish.

27p tunelove 10-06-2021 13 4   Download

Hepatitis B Infection is an occupational high risk factor. Immune response for vaccination was measured by Antibody to Hepatitis B surface antigen (anti HBs) levels. A course of Hep B vaccine yields a protective immune response (anti-HBs ≥10 mIU/mL) this study was designed to determine the anti HBs levels in Health Care Workers (HCWs) working as laboratory technician and staff nurses.

7p angicungduoc6 22-07-2020 23 3   Download

Regulator of G-protein signaling (RGS) proteins were originally identified as negative regulators of G-protein-coupled receptor (GPCR) signaling via their GTPase-accelerating protein (GAP) activity. All RGS proteins contain evolutionarily conserved RGS domain; however, they differ in their size and regulatory domains. RGS1 and RGS10 are smaller than other RGS proteins, and their functions involve various inflammatory responses including autoimmune responses in both the periphery and the central nervous system (CNS). Neuroinflammation is the chronic inflammatory response in the CNS.

5p caothientrangnguyen 09-05-2020 29 2   Download

Schistosomiasis is an endemic disease in Egypt caused by the trematode Schistosoma which has different species. c the best known form of chronic disease with a wide range of clinical manifestations. The pathogenesis of schistosomiasis is related to the host cellular immune response. This leads to granuloma formation and neo angiogenesis with subsequent periportal fibrosis manifested as portal hypertension, splenomegaly and esophageal varices. Intestinal schistosomiasis is another well identified form of chronic schistosomal affection.

8p kethamoi1 17-11-2019 30 2   Download

Cannabinoid receptors and their endogenous ligands, the endocannabinoids, have been detected in several blood immune cells, including monocytes/macrophages, basophils and lymphocytes. However, their presence in dendritic cells, which play a key role in the initiation and development of the immune response, has never been investigated.

8p system191 01-06-2013 29 4   Download

The hepatocyte growth factor-regulated tyrosine kinase substrate Hrs is an early endosomal protein that is thought to play a regulatory role in the trafficking of growth factor/ receptor complexes through early endosomes. Stimulation of cells with epidermal growth factor (EGF) rapidly leads to phosphorylation of Hrs, raising the question whether the receptor tyrosine kinase phosphorylates Hrs directly. Here, we present evidence that a downstream kinase, rather than the active receptor kinase is responsible. ...

7p research12 23-04-2013 38 2   Download

We have analyzed the response of a number of human cell lines to treatment with antisense oligodeoxynucleotides (ODNs) directed against RNA polymerase II, replication protein A, and Ha-ras. ODN-delivery to the cells was liposome-mediated or via electroporation, which resulted in di€erent intracellular locations of the ODNs. The ODN-mediated target mRNA reduction varied consider-ably between the cell lines.

10p research12 23-04-2013 42 3   Download

Basic ®broblast growth factor (bFGF) regulates diversi®ed biological functions in rat Sertoli cells. This report demon-strates that bFGF inhibits steroidogenesis in developing rat Sertoli cells. Follicle stimulating hormone (FSH)-stimulated estradiol production was reduced by bFGF. Moreover, the amount of cytochrome P450 aromatase, responsible for the irreversible transformation of androgens into estrogens, is decreased by bFGF at the transcriptional level.

10p research12 23-04-2013 31 2   Download

Drosophilais a powerful model system to study the regula-tory and effector mechanisms of innate immunity. To iden-tify molecules induced in the course of viral infection in this insect, we have developed a model based on intrathoracic injection of the picorna-like DrosophilaCvirus(DCV).We have used MALDI-TOF mass spectrometry to compare the hemolymph of DCV infected flies and control flies. By contrast with the strong humoral response triggered by injectionof bacteriaor fungal spores, we have identifiedonly one molecule induced in the hemolymph of virus infected flies....

10p tumor12 20-04-2013 27 3   Download

The mitogen activated protein (MAP) kinase module: (Raf fiMEKfiERKs) is central to the control of cell growth, cell differentiation and cell survival. The fidelity of signalling and the spatio-temporal activation are key deter-minants in generating precise biological responses. The fidelity is ensured by scaffold proteins – protein kinase
insulators – andby specific docking sites. The duration and the intensity of the response are in part controlled by the compartmentalization of the signalling molecules. ...

9p tumor12 20-04-2013 30 2   Download

Approximately 10% of amylolytic enzymes are able to bind and degrade raw starch. Usually a distinct domain, the starch-binding domain (SBD), is responsible for this property. These domains have been classified into families of carbohydrate-binding modules (CBM). At present, there are six SBD families: CBM20, CBM21, CBM25, CBM26, CBM34, and CBM41.

17p fptmusic 12-04-2013 38 4   Download

The Goodpasture antigen-binding protein, GPBP, is a serine⁄threonine kinase whose relative expression increases in autoimmune processes. Tumor necrosis factor (TNF) is a pro-inflammatory cytokine implicated in auto-immune pathogenesis. Here we show that COL4A3BP, the gene encoding GPBP, maps head-to-head withPOLK, the gene encoding for DNA polymerase kappa (polj), and shares with it a 140-bp promoter containing a Sp1 site, a TATA-like element, and a nuclear factor kappa B (NFjB)-like site

15p fptmusic 12-04-2013 36 3   Download

The induction of the heat shock response as well as its termination is auto-regulated by heat shock protein activities. In this study we have investi-gated whether Hsp90 functional protein levels influence the characteristics and duration of the heat shock response. Treatment of cells with several benzoquinone ansamycin inhibitors of Hsp90 (geldanamycin, herbimycin A) activated a heat shock response in the absence of heat shock, as reported previously.

13p fptmusic 12-04-2013 50 3   Download

The complex C1 triggers the activation of the Complement classical pathway through the recognition and binding of antigen–antibody complex by its subunit C1q. The globular region of C1q is responsible for C1 binding to the immune complex. C1q can also bind nonimmune molecules such as DNAand sulfated polysaccharides, leading either to the activation or inhibition of Complement. The binding site of these nonimmune ligands is debated in the literature, and it has beenproposed to be located either in the globular region or in the collagen-like regionof C1q, or inboth....

7p fptmusic 12-04-2013 41 2   Download

TheRel/NF-jBfamilyof transcription factorsplaykey roles in morphogenesis and immune responses. We reported previously that As-rel1 and As-rel2 of the ascidianHalo-cynthia roretziare involved in notochord formation. The As-rel1 protein is a typical Rel/NF-jB family member, whereas the As-rel2 protein is a novel truncated product of As-rel1 that lacks a nuclear localization signal and the unique C-terminal region. Here, we present conclusive evi-dence that As-rel1 and As-rel2 are generated from a single gene by alternative splicing....

10p fptmusic 12-04-2013 41 2   Download

In the horseshoe crab, bacterial lipopolysaccharide (LPS) induces exocyto-sis by granular hemocytes, resulting in the secretion of various defense molecules, such as lectins and antimicrobial peptides, via a G protein-mediating signaling pathway. This response is a key component of the horseshoe crab innate immune response against infectious microorganisms. Here, we report an endogenous amplification mechanism for LPS-induced hemocytes exocytosis.

9p fptmusic 11-04-2013 43 2   Download

Juvenile hormones (JHs) of insects are sesquiterpenoids that regulate a great diversity of processes in development and reproduction. As yet the molecular modes of action of JH are poorly understood. TheMethoprene-tolerant (Met) gene of Drosophila melanogasterhas been found to be responsible for resistance to a JH analogue (JHA) insecticide, methoprene. Previous studies onMethave implicated its involvement in JH signaling, although direct evidence is lacking.

0p awards 05-04-2013 33 1   Download

Formyl peptide receptor-like 1 (FPRL1) is a seven transmembrane domain, G protein-coupled receptor that interacts with a variety of exogenous and host-derived agonists. In order to identify domains crucial for ligand recog-nition by FPRL1, we used chimeric receptors with segments in FPRL1 replaced by corresponding amino acid sequences derived from the proto-type formyl peptide receptor FPR. The chimeric receptors were stably transfected into human embryonic kidney epithelial cells and the capacity of the cells to migrate in response to formyl peptide receptor agonists was evaluated. ...

10p awards 05-04-2013 36 3   Download

High-pressure NMR experiments performed on the histi-dine-containing phosphocarrier protein (HPr) fromSta-phylococcus carnosushave shown that residue Ile14, which is located in the active-centre loop, exhibits a peculiarly small pressure response. In contrast, the rest of the loop shows strong pressure effects as is expected for typical protein interaction sites. To elucidate the structural role of this residue, the mutant protein HPr(I14A), in which Ile14 is replaced by Ala, was produced and studied by solution NMR spectroscopy. ...

10p awards 05-04-2013 49 3   Download

We have shown recently that in human T lymphocytes, leptin stimulates activity and expression of the endocan-nabinoid-degrading enzyme fatty acid amide hydrolase (FAAH), through STAT3 (signal transducer and activator of transcription 3) and its CRE (cAMP response element)-like transcriptional target in the FAAH promoter [Maccar-rone, M., Di Rienzo, M., Finazzi-Agro`,A., &Rossi,A. (2003) J. Biol. Chem. 278, 13318–13324]. We have also shown that progesterone, alone or additively with leptin, up-regulates theFAAHgene in human T-cells, through the Ikaros transcription factor [Maccarrone, M.

11p awards 05-04-2013 37 3   Download