Induced mutation

An experiment was conducted to generate a broad genetic variability through induced mutation using physical and chemical mutagens. Three doses each of gamma rays (20, 40 and 60kR), ethyl methane sulphonate (0.2, 0.4 and 0.6%), nitrosoguanidine (0.005, 0.010, and 0.015%), maleic hydrazide (0.01, 0.02 and 0.03%) and their combinations were administered to the seed of two greengram varieties, Sujata and OBGG-52.

13p angicungduoc6 22-07-2020 20 2   Download

The development of fruits without fertilization is known as parthenocarpy. Parthenocarpy improves the fruit quality, processing attributes, production and productivity of vegetable crops like tomato, cucumber, watermelon etc. Absence of seeds can enhances the shelf life of the fruits, allowing a better conservation, fruit set in adverse climatic conditions, early and offseason production of vegetable crops. Therefore, it is important to ensure yield stability regardless of environmental conditions.

20p gaocaolon5 14-06-2020 16 3   Download

Increasing the productivity of rapeseed as one of the widely cultivated oil crops in the world is of upmost importance. As flowering time and plant architecture play a key role in the regulation of rapeseed yield, understanding the genetic mechanism underlying these traits can boost the rapeseed breeding

12p vishikamaru2711 29-04-2020 16 1   Download

General objective: To get mutant flowering flowers that have more number of petals than the study samples, larger flowers and more aromatic. To evaluate growth culture technique to generate calli and shoots for irradiation of gamma ray;

31p phongtitriet000 08-08-2019 33 4   Download

The K103N substitution is a frequently observed HIV-1 RT mutation in patients who do not respond to combinationtherapy. The drugs Efavirenz, MSC194 and PNU142721 belong to the recent generation of NNRTIs characterized by an improved resistance profile to the most common single point mutations within HIV-1 RT, including the K103N mutation. In the present study we present structural observations from Efavirenz in complex with wild-type protein and the K103N mutant and PNU142721 and MSC194 in complex with the K103N mutant.

8p system191 01-06-2013 41 4   Download

Group IID secretory phospholipase A2 (sPLA2-IID), a heparin-binding sPLA2 that is closely related to sPLA2-IIA, augments stimulus-induced cellular arachidonate release in a manner similar to sPLA2-IIA. Here we identified the residues of sPLA2-IID that are responsible for heparanoid binding, are and therefore essential for cellular function.

10p system191 01-06-2013 36 5   Download

We previously reported that GTS1 is involved in regulating ultradian oscillations of the glycolytic pathway induced by cyanide in cell suspensions as well as oscillations of energy metabolism in aerobic continuous cultures. Here, we screened a yeast cDNA library for proteins that bind to Gts1p using the yeast two-hybrid system and cloned multiple TDH cDNAs encoding the glycolytic enzyme glyceraldehyde-3-phosphate dehydrogenase (GAPDH).

10p research12 01-06-2013 40 3   Download

The Ca 2+ -induced transition in the troponin complex (Tn) regulates vertebrate striated muscle contraction. Tn was reconstituted with recombinant forms of troponin I (TnI) containing a single intrinsic 5-hydroxytryptophan (5HW). Fluorescence analysis of these mutants of TnI demonstrate that the regions in TnI that respond to Ca 2+ binding to the regulatoryN-domain of TnC are the inhibitoryregion (residues 96–116) and a neighboring region that includes position 121.

8p fptmusic 16-04-2013 39 4   Download

Guanylate cyclase-activating proteins (GCAPs) are neur-onal calcium sensors that activate membrane bound guanylate cyclases (EC 4.6.1.2.) of vertebrate photoreceptor cells when cytoplasmic Ca 2+ decreases during illumination. GCAPs contain fourEF-handCa 2+ -bindingmotifs, but the first EF-hand is nonfunctional. It was concluded that for GCAP-2, the loss of Ca 2+ -binding ability of EF-hand 1 resulted in a region that is crucial for targeting guanylate cyclase [Ermilov,A.N.,Olshevskaya, E.V.&Dizhoor,A.M. (2001) J. Biol. Chem.276, 48143–48148].

9p awards 05-04-2013 49 4   Download

The tissue-specific transcription factorsHNF1aandHNF1b are closely related homeodomain proteins conserved in vertebrate evolution. Heterozygous mutations in human HNF1b 1 but not inHNF1agenes are associated with kidney malformations. Overexpression of HNF1b in Xenopus embryos leads to defective pronephros development, while HNF1ahas no effect. We have defined the regions respon-sible for this functional difference between HNF1band HNF1ain transfected HeLa cells as well as in injected Xenopusembryos. ...

14p awards 05-04-2013 44 3   Download

Several oncogenic proteins are known to influence cellular glycosylation. In particular, transfection of codon 12 point mutated H-Ras increases CMP-Neu5Ac: Galb1,4GlcNAc a2,6-sialyltransferase I (ST6Gal I) activity in rodent fibroblasts. Given that Ras mediates its effects through at least three secondary effector pathways (Raf, RalGEFs and PI3K) and that transcriptional control ofmouse ST6Gal I is achieved by the selective use of multiple promoters, we attempted to identifywhichof these parameters are involved in linking the Ras signal to ST6Gal I gene transcription in mouse fibroblasts. ...

12p awards 05-04-2013 42 3   Download

We report here thatEscherichia coli pyrophosphatase aggregates in the presence of millimolar Cd 2+ . This highly cooperative process was specific to both the metal ion and the protein and could be reversed fully by decreasing the Cd 2+ concentration. Aggregation was enhanced by Mg 2+ , the natural cofactor of pyrophosphatase, and Mn 2+ . Mutations at the intersubunit metal-binding site had no effect, whereas mutation at Glu139, which is part of the peripheral metal-binding site found in pyrophosphatase crystals near the contact region between two enzyme mole-cules, suppressed aggregation.

0p awards 05-04-2013 34 2   Download

The information required for successful assembly of an icosahedral virus is encoded in the native conformation of the capsidproteinand in its interactionwith the nucleic acid. Here we investigated how the packing and stability of virus capsids are sensitive to single amino acid substitutions in the coat protein. Tryptophan fluorescence, bis-8-anilinonaph-thalene-1-sulfonate fluorescence, CD and light scattering were employed to measure urea- and pressure-induced effects on MS2 bacteriophage and temperature sensitive mutants. ...

11p dell39 03-04-2013 37 4   Download

CCN4⁄Wnt-induced secreted protein 1 (WISP1) is one of the CCN (CTGF⁄Cyr61⁄Nov) family proteins. CCN members have typical structures composed of four conserved cysteine-rich modules and their variants lack-ing certain modules, generated by alternative splicing or gene mutations, have been described in various pathological conditions.

11p galaxyss3 21-03-2013 37 4   Download

Rare, inherited mutations causing familial forms of Parkinson’s disease have provided insight into the molecular mechanisms that underlie the genetic and sporadic forms of this disease. Loss of protein function result-ing from autosomal-recessive mutations in PTEN-induced putative kinase 1 (PINK1), Parkin and DJ-1 has been linked to mitochondrial dysfunction, accumulation of abnormal and misfolded proteins, impaired protein clear-ance and oxidative stress.

9p vinaphone15 28-02-2013 39 3   Download

The chitosanase fromStreptomycessp. N174 (CsnN174) is an inverting glycoside hydrolase belonging to family 46. Previous studies identified Asp40 as the general base residue. Mutation of Asp40 into glycine revealed an unexpectedly high residual activity. D40G mutation did not affect the stereochemical mechanism of catalysis or the mode of interaction with sub-strate.

12p vinaphone15 27-02-2013 36 2   Download

Controlled activation of epidermal growth factor receptor (EGFR) is sys-tematically guaranteed at the molecular level; however, aberrant activation of EGFR is frequently found in cancer. Transcription induced by EGFR activation often involves the coordinated expression of genes that positively and negatively regulate the original signaling pathway; therefore, altera-tions in EGFR kinase activity may reflect changes in gene expression asso-ciated with the pathway.

13p vinaphone15 25-02-2013 34 5   Download

Angiogenin is a 14 kDa protein originally identified as an angiogenic pro-tein. Recent development has shown that angiogenin acts on both endothe-lial cells and neuronal cells. Loss-of-function mutations in the coding region of the ANGgene have recently been identified in patients with amyotrophic lateral sclerosis.

13p viettel02 19-02-2013 43 4   Download

IjB-f, an essential inflammatory regulator, is specifically induced by Toll-like receptor ligands or interleukin (IL)-1bby post-transcriptional acti-vation mediated via a 165-nucleotide element in IjB-f mRNA. Here, we analyzed the Toll-like receptor–IL-1 receptor signaling components involved in the post-transcriptional regulation of IjB-fwith mutated estro-gen receptor [ER(T2)] fusion proteins.

12p cosis54 08-12-2012 37 2   Download

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: TILLING to detect induced mutations in soybean

10p panasonic06 26-12-2011 49 7   Download