Isolation and molecular identification

Bacterial strain LB7 isolated from pickled melon solution was identified as Lactobacillus plantarum using molecular marker 16S rRNA gene (rDNA) sequences. The content of lactic acid in the isolate culture was relatively high (33.7 g/l) when quantified using HPLC system.

7p vimarillynhewson 02-01-2024 2 0   Download

Environments with temperatures from 50°C to 80°C are rare in nature and are almost exclusively associated with geothermal regions including hot springs, solar-heated soils and volcanic areas. Thermophilic bacteria already exist and prefer in such habitats.

9p vimulcahy 18-09-2023 6 3   Download

In this study, species DL0091 was morphologically and phylogenetically described as Metacordyceps neogunnii, containing numerous bioactive constituents, especially a high amount of adenosine and cordycepin, therefore, it was considered a valuable resource in medicine.

12p vimulcahy 18-09-2023 6 3   Download

The aim of this study was to obtain quantitative data about the frequency, genotypic characterization and antibiotic resistance profiling of Salmonella agents in chicken flocks located in eastern Turkey.

8p vigandhi 23-02-2022 12 1   Download

Gonadal sex differentiation proceeds by the interplay of various genes including the transcription factors and secretory factors in a complex network. Dmrt1 and Sox9 were considered to be the essential genes involved in the testicular differentiation in mammals, birds, reptiles, and fishes. Here, we have successfully isolated and characterized cDNAs encoding the Rana chensinensis orthologs of Dmrt1 and Sox9, and we have determined patterns of expression during gonadal differentiation and adult tissues.

12p dolomite36 30-12-2021 11 0   Download

A new microsporidian pathogen isolated from Crepidodera aurata was identified based on morphological and ultrastructural characteristics, coupled with a molecular phylogenetic analysis. The spores of the microsporidian pathogen were slightly curved in shape, and measured 2.44–3.55 µm in length and 1.25–1.55 µm in width (n = 50). Its ultrastructure is characteristic of monokaryotic groups. All lifecycle stages of the pathogen, including meronts, sporonts, sporoblasts, and mature spores, are monokaryotic. The spore has 6–8 windings of the polar filament.

9p dolomite36 30-12-2021 9 0   Download

The rose sawfly, Arge rosae L. (Hymenoptera: Argidae), is one of the most destructive pests of roses grown both indoors and outdoors in Antalya province (south-western part of Turkey). Especially in greenhouse rose cultivation, growers depend heavily on synthetic pesticides to control this pest along with other arthropod pests in their crops. The aim of this study was to evaluate biocontrol potential of some indigenous soil-borne entomopathogenic fungi (EPF) against the pest. In pathogenicity assays, a total of 17 EPF isolates, belonging to three species [Beauveria bassiana (Bals.) Vuill.

9p dolomite36 30-12-2021 8 0   Download

The present researchaimed to investigate the morphological, biochemical and genotypic characterization of dominant lactic acid bacteria that were isolated from traditional dairy products in Iranian tribes . A total of 75 samples of traditional yogurt, doogh from dairy units in Fars province were randomly collected. Isolation of lactic acid bacteria, biochemical and genotypic identification were conducted. Totally, 157 LAB isolates were selected.

6p mudbound 10-12-2021 27 1   Download

Molecular knowledge regarding the primary esophageal achalasia is essential for the early diagnosis and treatment of this neurodegenerative motility disorder. Therefore, there is a need to find the main microRNAs (miRNAs) contributing to the mechanisms of achalasia. This study was conducted to determine some patterns of deregulated miRNAs in achalasia. This case-control study was performed on 52 patients with achalasia and 50 nonachalasia controls. The miRNA expression profiling was conducted on the esophageal tissue samples using the next-generation sequencing (NGS).

13p thiencuuchu 27-11-2021 7 1   Download

Campylobacter species are a leading cause of food-borne disease and C. jejuni highlight the most potential public health impact of Campylobacter contamination by pathogens originating from animals or animal products.. The total of 33meat samples comprising 8 from sheep (5) and goat (3) also 25 beef samples were screened by morphological, biochemical and molecular technique.

9p chauchaungayxua11 23-03-2021 15 2   Download

Nigrospora sphaerica (Sacc.) Mason. found to infect leaves of Cajanus cajan L. in the Marathwada region of Maharashtra, India is a new host record. Symptoms on leaves appeared in the form of semi circular to irregular, grey to black coloured spots. Based on morphological, cultural and molecular study. The fungus isolated was identified as Nigrospora sphaerica Mason. Pathogenecity test of the fungus was confirmed as per Koch's postulates.

4p chauchaungayxua11 23-03-2021 13 3   Download

An increasing interest has emerged with respect to the importance of microbial diversity in soil habitats. The extent of the diversity of microorganisms in soil is seen to be critical to the maintenance of soil health and quality, as a wide range of microorganisms is involved in important soil functions.

7p cothumenhmong9 04-01-2021 6 1   Download

Cellulolytic bacteria were previously isolated from the termite guts and efforts were taken to optimize parameters for cellulase production. Activities of diversity of enzymes which include, CMCase, avicelase and filterpaperase (FPase) were studied from crude cellulase extracts. CMCase activity was found optimum at different temperatures (35°C, 40°C and 45°C) at pH 7 and after 48 hrs of incubation time.

19p gaocaolon9 22-12-2020 5 1   Download

The present investigation 610 serum samples, abomasal contents and tissue samples from 5 aborted fetuses were collected from dairy farms of cattle and buffalo in and around Mumbai and Pune region.

12p gaocaolon9 22-12-2020 6 1   Download

The aim is to diagnose Dengue infection, its local epidemiology, seroprevalance and determination in Thanjavur district by viral isolation using cell line, serological tests, and molecular methods and genome sequencing.

7p gaocaolon9 22-12-2020 9 2   Download

Present experiment formulated to study bacterial decolourization of Red HE7B dye with identification and molecular characterization by RAPD.

7p gaocaolon9 22-12-2020 8 2   Download

Keeping in view above considerations, the present study was framed for isolation, biochemical characterization and molecular identification of iron nanoparticles synthesizing bacteria by use of 16S rRNA technology to determine evolutionary rates of different bacterial isolates and close synteny between them by using various bioinformatics tools.

12p angicungduoc8 07-11-2020 26 3   Download

Identification of bacteria may be based on sequencing and molecular analysis of a specific locus such as 16S rRNA, or a set of loci such as in multilocus sequence typing. In the near future, healthcare institutions and routine diagnostic microbiology laboratories may need to sequence the entire genome of microbial isolates.

10p vioklahoma2711 19-11-2020 19 1   Download

In this study, we used traditional methods to isolate and screen for fungal laccases that have potential for use in the textile industry (dye decolorization) and a molecular marker to identify studied samples. Fifteen strains of basidiomycetes were isolated and screened for laccase activities on phenol, guaiacol and RBBR. Five strains showed positive laccase activities on all three substrates.

7p tamynhan8 04-11-2020 7 1   Download

The aim of this study was to isolate, characterize and identify endophytic bacteria from plants growing along the stream banks in Hot Springs, South Dakota. The bacterial endophytes were isolated, identified and screened in vitrofor morphological features (Gram stain, Gram morphology, and colony morphology). Further, isolates exhibiting difference in morphological features were selected for molecular identification through partial 16S-rRNA gene sequencing.

12p trinhthamhodang1215 23-09-2020 13 2   Download