Medical common knowledge

This research project aimed to: (a) determine the demographic profile and common conditions of patients in a Teaching Clinic; (b) describe the adverse events from Chinese medicine treatment; (c) examine the overall treatment outcomes and treatment interventions of Chinese medicine in patients with respiratory disorders; and (d) assess the level of knowledge and compliance with Chinese medicine in patients with respiratory disorders.

304p runthenight07 01-03-2023 9 3   Download

Two neuropeptides have been isolated and identi®ed from the secretions of the skin glands of the Stony Creek Frog Litoria lesueuri. The ®rst of these, the known neuropeptide caerulein 1.1, is a common constituent of anuran skin secretions, and has the sequence pEQY(SO3 )TGWMDF-NH2 . This neuropeptide is smooth muscle active, an anal-gaesic more potent thanmorphine and is also thought to be a hormone.

10p research12 29-04-2013 41 3   Download

This paper describes violaxanthin de-epoxidation in model lipid bilayers. Unilamellar egg yolk phosphatidylcholine (PtdCho) vesicles supplemented with monogalactosyldi-acylglycerol were found to be a suitable system for studying this reaction. Such a system resembles more the native thylakoid membrane and offers better possibilities for studying kinetics and factors controlling de-epoxidation of violaxanthin than a systemcomposed only of monogalacto-syldiacylglycerol and is commonly used in xanthophyll cycle studies. ...

10p research12 23-04-2013 30 3   Download

DNA ligases are the enzymes responsible for the repair of single-strandedanddouble-strandednicks indsDNA.DNA ligases are structurally similar, possibly sharing a common molecular mechanism of nick recognition and ligation catalysis. This mechanism remains unclear, in part because the structure of ligase in complex with dsDNAhas yet to be solved.DNA ligases share common structural elementswith DNA polymerases, which have been cocrystallized with dsDNA. Based on the observed DNA polymerase–dsDNA interactions, we propose a mechanism for recognition of a single-stranded nick by DNA ligase....

7p tumor12 22-04-2013 39 2   Download

Thehemolysateofthe AntarcticteleostGobionotothen gibberifrons(family Nototheniidae) contains two hemoglo-bins (Hb 1 andHb 2). The concentration of Hb 2 (15–20% of the total hemoglobin content) is higher than that found in most cold-adapted Notothenioidei. Unlike the other Antarctic species so far examined having two hemoglobins, Hb 1 and Hb 2 do not have globin chains in common. Therefore this hemoglobin system is made of four globins (two a-and twob-chains). The complete amino-acid sequence of the two hemoglobins (Hb 1,a 1 2b 1 2 ;Hb 2,a 2 b 2 2 ) has been established. ...

7p tumor12 20-04-2013 27 4   Download

We have compared the site-by-siteN-glycosylation status of human lactoferrin (Lf) produced in maize, a monocotyle-don, and in tobacco, usedas amodel dicotyledon.Maizeand tobacco plants were stably transformed and recombinant Lf was purified from both seeds and leaves. N-glycopeptides were generated by trypsin digestion of recombinant Lf and purified by reverse-phase HPLC. The N-glycosylation pat-tern of each site was determined by mass spectrometry. Our results indicated that the N-glycosylation patterns of recombinant Lf produced in maize and tobacco share common structural features....

8p tumor12 20-04-2013 43 2   Download

Structural analysis of the lipopolysaccharide (LPS) from nontypeableHaemophilus influenzaestrain 981 has been achieved using NMRspectroscopy and ESI-MS on O-deacylatedLPSand core oligosaccharide (OS)material as well as by ESI-MS n on permethylated dephosphorylated OS. A heterogeneous glycoform population was identified, resulting from the variable length of the OS branches attached to the glucose residue in the common structural element of H. influenzae LPS, L-a-D-Hepp-(1fi2)-[PEtnfi6]-L-a-D-Hepp-(1fi3)-[b-D-Glcp-(1fi4)]-L-a-D-Hepp-(1fi5)-[PPEtnfi4]-a-Kdop-(2fi6)-Lipid A....

13p fptmusic 16-04-2013 44 2   Download

Interleukin (IL)-10 was cloned from the common carp (Cyprinus carpioL.) using IL-10 primers from carp head kidney following stimulation with concanavalin A and lipopolysaccharide. The cDNA consisted of a 1096 bp se-quence containing a 55 bp 5¢ untranslated region and a 498 bp 3¢ untranslated region. An open reading frame of 543 bp encoded a putative 180 amino acid protein with a putative signal peptide of 22 amino acids.

8p fptmusic 12-04-2013 31 3   Download

Ultrasensitive responses are common in cellular information transfer because they allow cells to decode extracellular stimuli in an all-or-none manner. Biochemical responses are usually analyzed by fitting the Hill equation, and the estimated Hill coefficient is taken as a measure of sensi-tivity. However, this approach is not appropriate if the response under con-sideration significantly deviates from the best-fit Hill equation.

9p fptmusic 11-04-2013 30 2   Download

The cAMP response element consensus sequence directs the transcription of a wide range of genes. A 24-mer single-stranded cAMP response element decoy oligonucleotide (CDO) has been shown to compete with these sequences for binding transcription factors and therefore interferes with cAMP-induced gene transcription. We have examined the effect of this CDOalone and in combinationwith a range of common chemotherapeutic agents in colorectal cancer cell lines.

9p dell39 03-04-2013 40 6   Download

NontypeableHaemophilus influenzae(NTHi) is a common commensal of the human upper respiratory tract and is associatedwithotitismedia in children. The structures of the oligosaccharide portions of NTHi lipopolysaccharide (LPS) from several otitis media isolates are now well characterized but it is not knownwhether there are structural differences in LPSfrom colonizing, nondisease associated strains.

13p dell39 03-04-2013 39 3   Download

Among the RFamide peptide groups, PQRFamide peptides, such as neuro-peptide FF (NPFF) and neuropeptide AF (NPAF), share a common C-ter-minal Pro-Gln-Arg-Phe-NH2 motif. LPXRFamide (X¼L or Q) peptides, such as gonadotropin-inhibitory hormone (GnIH), frog growth hormone-releasing peptide (fGRP), goldfish LPXRFamide peptide and mammalian RFamide-related peptides (RFRPs), also share a C-terminal Leu-Pro-Leu⁄Gln-Arg-Phe-NH2 motif.

13p inspiron33 26-03-2013 41 5   Download

Fatty acid-binding protein type 1 (FABP1), commonly termed liver-type fatty acid-binding protein (L-FABP), is encoded by a single gene in mam-mals. We cloned and sequenced cDNAs for two distinct FABP1s in zebra-fish coded by genes designatedfabp1a andfabp1b. The zebrafish proteins, FABP1a and FABP1b, show highest sequence identity and similarity to the human protein FABP1.

14p inspiron33 25-03-2013 39 2   Download

Lithium is a therapeutic agent commonly used to treat bipolar disorder and its beneficial effects are thought to be due to a combination of activa-tion of the Wnt⁄b-catenin pathway via inhibition of glycogen synthase kin-ase-3b and depletion of the inositol pool via inhibition of the inositol monophosphatase-1.

17p galaxyss3 19-03-2013 24 3   Download

The methionine salvage pathway is universally used to regenerate methio-nine from 5¢-methylthioadenosine, a byproduct of certain reactions involv-ing S-adenosylmethionine. We identified and verified the genes encoding the enzymes of all steps in this cycle in a commonly used eukaryotic model system: the yeast Saccharomyces cerevisiae.

10p galaxyss3 07-03-2013 50 3   Download

Rare, inherited mutations causing familial forms of Parkinson’s disease have provided insight into the molecular mechanisms that underlie the genetic and sporadic forms of this disease. Loss of protein function result-ing from autosomal-recessive mutations in PTEN-induced putative kinase 1 (PINK1), Parkin and DJ-1 has been linked to mitochondrial dysfunction, accumulation of abnormal and misfolded proteins, impaired protein clear-ance and oxidative stress.

9p vinaphone15 28-02-2013 39 3   Download

Receptors should be properly analysed in view of the microenvironment in which they are embedded. Therefore, the concept of ‘receptosome’ was for-mulated to the complex interactions taking place between receptors and other proteins at the plasma membrane level, and to explain very heteroge-neous or divergent cellular responses to common epigenetic factors and modifications to the extracellular environment.

12p vinaphone15 27-02-2013 42 2   Download

Mixed lineage leukemias (MLLs) are histone-methylating enzymes with critical roles in gene expression, epigenetics and cancer. Although MLLs are important gene regulators little is known about their own regulation. Herein, to understand the effects of toxic stress onMLLgene regulation, we treated human cells with a common food contaminant mycotoxin, deoxynivalenol (DON).

9p viettel02 22-02-2013 33 3   Download

Chitotriosidase (CHIT1) is a chitinase that is secreted by activated macro-phages. Plasma chitotriosidase activity reflects the presence of lipid-laden macrophages in patients with Gaucher disease. CHIT1 activity can be con-veniently measured using fluorogenic 4-methylumbelliferyl (4MU)–chitotri-oside or 4MU–chitobioside as the substrate, however, nonsaturating concentrations have to be used because of apparent substrate inhibition.

11p viettel02 20-02-2013 27 1   Download

Aldo-keto reductase 1B1 and 1B3 (AKR1B1 and AKR1B3) are the pri-mary human and mouse prostaglandin F2a(PGF2a) synthases, respectively, which catalyze the NADPH-dependent reduction of PGH2, a common intermediate of various prostanoids, to form PGF2a. In this study, we found that AKR1B1 and AKR1B3, but not AKR1B7 and AKR1C3, also catalyzed the isomerization of PGH2 to PGD2 in the absence of NADPH or NADP + .

11p cosis54 05-01-2013 37 5   Download