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Mouse embryo
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Ebook "p53 Protocols (Second edition)" expert researchers in the field detail many of the methods that address the challenging questions of the p53 field. These methods include the identification of the target genes and binding partners of gain of function p53 mutants, methods to determine stress response, autophagy or senescence induced by p53, cell cycle analysis, analysis of different phases of DNA replication modified by p53, and generation of pluripotent stem cells.
251p
dongmelo
27-05-2024
3
2
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The diagnostic rate of Mendelian disorders in sequencing studies continues to increase, along with the pace of novel disease gene discovery. However, variant interpretation in novel genes not currently associated with disease is particularly challenging and strategies combining gene functional evidence with approaches that evaluate the phenotypic similarities between patients and model organisms have proven successful.
20p
viellison
28-03-2024
1
1
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Ebook "Zygotic genome activation: Methods and protocols" provides an overview of zygotic genome activation (ZGA) and the use of recent tools to elucidate the events that occur during ZGA. The chapters in this book cover topics such as: clearance of maternal RNAs, detection of miRNA in mammalian oocytes and embryos, RNA FISH to study ZGA in early mouse embryos, and CRISPR/Cas9-mediated gene targeting during embryogenesis in the mouse and pig.
271p
lucchinguyen
28-12-2023
6
2
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Part 1 book "Transgenic mouse - Methods and protocols" includes content: Introduction - Strategies for developing genetically modified mice; genetic modification of the mouse - general technology – pronuclear and blastocyst injection; generation of chimeras by aggregation of embryonic stem cells with diploid or tetraploid mouse embryos; cryopreservation of mouse spermatozoa and in vitro fertilization; autopsy and histological analysis of the transgenic mouse; transgene design; inducible transgenic mouse models; lentiviral transgenesis; transgenesis in mouse embryonic stem cells.
175p
oursky09
12-11-2023
4
3
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Part 1 book "RT-PCR Protocols" includes content: Single cell RT-PCR on mouse embryos - A general approach for developmental biology; detection of influenza a virus neuraminidase and pb2 gene segments by one step reverse transcription polymerase chain reaction; RT-PCR amplification and cloning of large viral sequences,... and other contens.
189p
oursky05
20-09-2023
1
0
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Part 1 book "Transgenic mouse, methods and protocols" includes content: Introduction - Atrategies for developing genetically modified mice; genetic modification of the mouse - general technology – pronuclear and blastocyst injection; generation of chimeras by aggregation of embryonic stem cells with diploid or tetraploid mouse embryos; cryopreservation of mouse spermatozoa and in vitro fertilization,.... and other contents.
175p
oursky05
20-09-2023
3
1
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Part 1 book "Avian embryology" includes content: Operations on primitive streak stage avian embryos; quail–chick transplantations, other chimeras - quail–duck and mouse–chick; manipulations of neural crest cells or their migratory pathways; embryo slices and strips - guidance and adhesion assays in the avian embryo; embryo slices and strips - guidance and adhesion assays in the avian embryo; cell division, diverentiation, and death in avian embryos;.... and other contents.
227p
oursky05
20-09-2023
2
2
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In higher vertebrates the notochord is a transient rod-like structure in the midline of the embryo. In mouse embryos beginning at embryonic stage E8.5 the notochord arises as a distinct anatomical entity. Its anterior end is close to Radtke’s pouch from which it extends posteriorly to the tip of the tail.
17p
vinarcissa
21-03-2023
1
1
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In this research, we evaluated the preimplantation development, embryo morphology, the time of cell division and the cell number of the blastocyst stage of single mouse blastomeres biopsied from 8-cell embryos. One blastomere was biopsied from each 8-cell embryo using micromanipulation system with XYClone laser.
8p
vimalfoy
08-02-2023
1
1
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This study examined the effects of Scriptaid, a histone deacetylase inhibitor (HDAC) during the first mitotic cell cycle on preimplantation development of mouse embryos generated by injection of round spermatid into mature oocytes.
8p
vimalfoy
08-02-2023
4
1
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Prime editors, novel genome-editing tools consisting of a CRISPR-Cas9 nickase and an engineered reverse transcriptase, can induce targeted mutagenesis. Nevertheless, much effort is required to optimize and improve the efficiency of prime-editing.
11p
viarchimedes
26-01-2022
8
0
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Transposable elements (TEs) are a significant component of eukaryotic genomes and play essential roles in genome evolution. Mounting evidence indicates that TEs are highly transcribed in early embryo development and contribute to distinct biological functions and tissue morphology.
25p
viarchimedes
26-01-2022
6
0
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In this study, three alkaloid fractions from Huperzia serrata (Thunb.) Trevis, Lycopodium clavatum L. and Huperzia squarrosa (G. Forst.) Trevis were tested in two toxicity models: mouse primary neuronal cell (in vitro) and zebrafish (Danio rerio) embryos (in vivo).
9p
guitaracoustic02
08-12-2021
15
1
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The high-density lipoprotein receptor SR-B1 mediates cellular uptake of several lipid species, including cholesterol and vitamin E. During early mouse development, SR-B1 is located in the maternal-fetal interface, where it facilitates vitamin E transport towards the embryo.
15p
vitzuyu2711
29-09-2021
8
1
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Long non-coding RNAs (lncRNAs), a type of epigenetic regulator, are thought to play important roles in embryonic development in mice, and several developmental defects are associated with epigenetic modification disorders.
13p
vitzuyu2711
29-09-2021
19
1
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While DNA and RNA methods are routine to disrupt the expression of specific genes, complete understanding of developmental processes requires also protein methods, because: oocytes and early embryos accumulate proteins and these are not directly affected by DNA and RNA methods.
19p
visilicon2711
20-08-2021
11
1
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Genetic studies in mouse have demonstrated the crucial function of PAX4 in pancreatic cell differentiation. This transcription factor specifies β- and δ-cell fate at the expense of α-cell identity by repressing Arx gene expression and ectopic expression of PAX4 in α-cells is sufficient to convert them into β-cells.
16p
vimariana2711
22-12-2020
7
0
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Morphometric quantification of subtle craniofacial variation in studies of experimentally modified embryonic mice has proved valuable in determining the effects of developmental perturbations on craniofacial morphogenesis.
12p
vimariana2711
22-12-2020
7
1
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The sub-cortical maternal complex (SCMC), located in the subcortex of mouse oocytes and preimplantation embryos, is composed of at least four proteins encoded by maternal effect genes: OOEP, NLRP5/ MATER, TLE6 and KHDC3/FILIA.
13p
vimariana2711
22-12-2020
9
1
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Definitive endoderm (DE) gives rise to the respiratory apparatus and digestive tract. Sox17 and Cxcr4 are useful markers of the DE. Previously, we identified a novel DE marker, Decay accelerating factor 1(Daf1/CD55), by identifying DE specific genes from the expression profile of DE derived from mouse embryonic stem cells (ESCs) by microarray analysis, and in situ hybridization of early embryos.
9p
viwyoming2711
16-12-2020
14
1
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