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Multiplex PCR analysis
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In this study, we utilize the genome-wide singlenucleotide polymorphisms (SNPs) data obtained through Multiplexed Inter Simple Sequence Repeat Genotyping by sequencing (MIG-seq) to explore the phylogenetic relationships among Quercus species in Vietnam. The results of this study reveal that all Quercus species in Vietnam belong to subgenus Cerris and the phylogenetic analysis strongly supports the recognition of two infrageneric sections: Quercus and Ilex section for the Vietnamese Quercus.
10p
dianmotminh02
03-05-2024
2
1
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Part 2 book "PCR Technology - Current innovations" includes content: Assay design for Real-Time qPCR, primer design for large scale multiplex PCR and arrayed primer extension, hypothesis driven approaches to multivariate analysis of qPCR data, Experiment Design, data management, and univariate statistical analysis of gene expression data obtained by real time quantitative PCR,.... and other contents.
261p
oursky06
17-10-2023
3
1
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We describe a highly sensitive, quantitative, and inexpensive technique for targeted sequencing of transcript cohorts or genomic regions from thousands of bulk samples or single cells in parallel. Multiplexing is based on a simple method that produces extensive matrices of diverse DNA barcodes attached to invariant primer sets, which are all pre-selected and optimized in silico.
16p
vielonmusk
30-01-2022
21
0
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Analysis of polymorphic microsatellite markers (STR) is a helpful genotyping technique to differentiate Candida parapsilosis sensu stricto isolates. The aim of this study is to develop and perform an initial validation of an alternative protocol for the reliable and accurate microsatellite genotyping of C. parapsilosis sensu stricto isolates using high-throughput multiplex PCR.
10p
vitzuyu2711
29-09-2021
11
1
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The methodology used was quite systematic, rapid, reliable, and appropriate for qualitative detection and can be extended for multiplex development in other plant species having SSR marker technology for efficient plant genetic resources management including genetic diversity analysis, cultivar identification and evolutionary studies in plants.
9p
chauchaungayxua10
19-03-2021
10
1
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Targeted resequencing with high-throughput sequencing (HTS) platforms can be used to efficiently interrogate the genomes of large numbers of individuals. A critical issue for research and applications using HTS data, especially from long-read platforms, is error in base calling arising from technological limits and bioinformatic algorithms.
8p
viconnecticut2711
28-10-2020
9
0
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Quantitative methylation-specific PCR (qMSP) analysis for determining the methylation status of (candidate) tumor suppressor genes has potential as objective and valuable test to triage high-risk human papillomavirus (hrHPV) positive women in cervical screening.
9p
vijisoo2711
30-09-2020
13
0
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Interrogate the impact of IKZF1 deletion on therapy-outcomes of adults with common B-cell acute lymphoblastic leukemia. Methods: One hundred sixty-five consecutive adults with common B-cell ALL were tested for IKZF1 deletion and for BCR/ABL. Deletions in IKZF1 were detected using multiplex RQ-PCR, multiplex fluorescent PCR, sequence analysis and multiplex ligation-dependent probe amplification (MLPA).
8p
vinaypyidaw2711
26-08-2020
11
1
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With the introduction of Olaparib treatment for BRCA-deficient recurrent ovarian cancer, testing for somatic and/or germline mutations in BRCA1/2 genes in tumor tissues became essential for treatment decisions.
15p
vikuala271
13-06-2020
13
1
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Photoperiod and/or thermo-sensitive male sterility is an effective pollination control system in crop two-line hybrid breeding. We previously discovered the spontaneous mutation of a partially male sterile plant and developed a thermo-sensitive genic male sterile (TGMS) line 373S in Brassica napus L.
14p
viuchiha2711
21-04-2020
9
2
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Amoebiasis, caused by Entamoeba histolytica, remains a serious public health issue worldwide. For decades, microscopy remains preferred method for detection of the pathogenic species. The recent advent of molecular biology has helped in developing appropriate diagnostic technologies, of which isothermal amplification is considered to be robust and cost-effective compared to PCR that requires expensive instruments and highly skilled personnel.
6p
quenchua4
06-04-2020
12
2
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T A total of 23 putative Listeria isolates obtained from different sources, viz. food, animal, human, caterpillar and mosquito were screened for presence of the virulence factors by multiplex polymerase chain reaction (mPCR). Multiplex polymerase chain reaction for the amplification of isp and prs genes was employed for genus and species identification, while virulence profiling was employed by amplification of plcA, hlyA, actA, prfA, inlC, inlJ, luxS and fla genes. All strains harbours virulence genes plcA, hlyA, actA, prfA, inlC, inlJ, luxS and fla.
8p
nguathienthan2
25-12-2019
11
0
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Multiplex reverse transcriptase–polymerase chain reaction (MRT-PCR) is a method of choice for efficient and simultaneous analysis of gene expression at the transcript level with limited amounts of tissues; however, it is less common than other methods due to certain limitations.
8p
vikimsa
22-02-2019
22
0
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