Nonribosomal peptide synthetase

Luận văn được thực hiện với mục tiêu nhằm phân lập và đánh giá đa dạng xạ khuẩn nội sinh trên cây Màng tang thu thập tại tỉnh Phú Thọ; đánh giá khả năng kháng vi sinh vật kiểm định các chủng xạ khuẩn nội sinh và xác định sự có mặt của ba gen mã hóa các enzyme tham gia vào quá trình tổng hợp kháng sinh gồm polyketide synthases I (PKS-I), polyketide synthases II (PKS-II) và nonribosomal peptide synthetase (NRPS). Mời các bạn cùng tham khảo.

67p zhangyan 13-07-2021 34 6   Download

Nghiên cứu này tập trung vào định danh, đánh giá hoạt tính kháng VSV kiểm định và xác định sự có mặt của các gen chức năng liên quan đến sinh kháng sinh, sinh kháng sinh nhóm anthracyclin của chủng xạ khuẩn YBQ75 nội sinh phân lập từ cây quế (Cinnamomum cassia Presl) tại tỉnh Yên Bái.

7p viathena2711 08-10-2019 56 4   Download

Many pharmacologically important agents are assembled on multimodular nonribosomal peptide synthetases (NRPSs) whose modules comprise a set of core domains with all essential catalytic functions necessary for the incorporation and modification of one building block. Very often, d-amino acids are found in such products which, with few exceptions, are generated by the action of NRPS integrated epimerization (E) domains that alter the stereochemistry of the corresponding peptidyl carrier protein (PCP) bound l-intermediate....

15p fptmusic 12-04-2013 30 2   Download

Microorganisms produce a large number of pharmaco-logically and biotechnologically important peptides by using nonribosomal peptide synthetases (NRPSs). Due to their modular arrangement and their domain organization NRPSs are particularly suitable for engineering recom-binant proteins for the production of novel peptides with interesting properties.

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This work presents a computational analysis of the molecular characteris-tics shared by the adenylation domains from traditional nonribosomal pep-tide synthetases (NRPSs) and the group of the freestanding homologous enzymes: a-aminoadipate semialdehyde dehydrogenase, a-aminoadipate reductase and the protein Ebony. The results of systematic sequence com-parisons allow us to conclude that a specificity-conferring code, similar to that described for the NRPSs, can be recognized in such enzymes.

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Recent studies on type II thioesterases (TEIIs) involved in microbial secondary metabolism described a role for these enzymes in the removal of short acyl-S- phosphopantetheine intermediates from misprimed holo-(acyl carrier proteins) and holo-(peptidyl carrier proteins) of polyketide synthases and nonribosomal peptide synthetases.

10p dell39 03-04-2013 53 4   Download

Aspergillus fumigatusis an important human fungal pathogen. The Asper-gillus fumigatus genome contains 14 nonribosomal peptide synthetase genes, potentially responsible for generating metabolites that contribute to organismal virulence. Differential expression of the nonribosomal peptide synthetase gene,pes1, in four strains of Aspergillus fumigatuswas observed.

16p inspiron33 25-03-2013 31 4   Download

The family of chromodepsipeptides constitutes a class of structurally related pseudosymmetrical peptidolactones and peptidothiolactones synthe-sized by nonribosomal peptide synthetases. The chromodepsipeptides, which are analogous to the extensively characterized echinomycin, attain their DNA-bisintercalating properties from chromophore moieties attached to the N-termini of the oligopeptide chain.

13p vinaphone15 27-02-2013 34 2   Download

Nonribosomal peptide synthetases serve as multidomain protein templates for producing a wealth of pharmaceutically important natural products. For the correct assembly of the desired natural product the interactions between the different catalytic centres and the reaction intermediates bound to the peptidyl carrier protein must be precisely controlled at spatial and temporal levels.

13p mobifone23 18-01-2013 41 3   Download