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Báo cáo khoa học: Mutational analysis of a type II thioesterase associated with nonribosomal peptide synthesis

Chia sẻ: Nguyen Thang | Ngày: | Loại File: PDF | Số trang:10

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Recent studies on type II thioesterases (TEIIs) involved in microbial secondary metabolism described a role for these enzymes in the removal of short acyl-S- phosphopantetheine intermediates from misprimed holo-(acyl carrier proteins) and holo-(peptidyl carrier proteins) of polyketide synthases and nonribosomal peptide synthetases. Because of the absence of structural information on this class of enzymes, we performed a mutational analysis on a prototype TEII essential for efficient productionof the lipopeptide antibiotic surfactin (TEIIsrf), which led to identificationof catalytic and structural residues. ...

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Nội dung Text: Báo cáo khoa học: Mutational analysis of a type II thioesterase associated with nonribosomal peptide synthesis

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