Báo cáo khoa học: Mutational analysis of substrate recognition by human arginase type I ) agmatinase activity of the N130D variant
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Upon mutation of Asn130 to aspartate, the catalytic activity of human arginase I was reduced to17% of wild-type activity, theKmvalue for arginine was increased9-fold, and thekcat ⁄Kmvalue was reduced50-fold. The kinetic properties were much less affected by replacement of Asn130 with glutamine.
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