Plant protein analysis

Two ORFs, cphA and cphB, encoding proteins CphA and CphB with strong similarities to plant phytochromes and to the cyanobacterial phytochrome Cph1 of Synechocystis sp. PCC 6803 have been identified in the filamentous cyanobacterium Calothrix sp. PCC7601. While CphA carries a cysteine within a highly conserved amino-acid sequence motif, to which the chromophore phytochromobilin is covalently bound in plant phytochromes, in CphB this position is changed into a leucine.

10p system191 01-06-2013 39 4   Download

Chimeric calcium/calmodulin dependent protein kinase (CCaMK) is characterized by the presence of a visinin-like Ca 2+ -binding domain unlike other known calmodulin-dependent kinases. Ca 2+ -Binding to the visinin-like domain leads to autophosphorylation and changes in the affinity for calmodulin [Sathyanarayanan P.V., Cremo C.R. & Poovaiah B.W. (2000)J. Biol. Chem. 275, 30417–30422]. Here, we report that the Ca 2+ -stimulated autophosphory-lation of CCaMK results in time-dependent loss of enzyme activity. ...

7p research12 29-04-2013 31 3   Download

A number of proteins related to the homotetrameric trans-port protein transthyretin (TTR) forms a highly conserved protein family, whichwe present in an integrated analysis of data from different sources combined with an initial bio-chemical characterization.Homologues of the transthyretin-relatedprotein (TRP) canbe found inawide rangeof species including bacteria, plants and animals, whereas transthyre-tinshave so faronlybeen identified invertebrates.

15p tumor12 20-04-2013 44 2   Download

Subunit G of photosystem I is a nuclear-encoded protein, predicted to form two transmembrane a-helices separated by a loop region. We use in vitro import assays to show that the positively charged loop domain faces the stroma, whilst the N- and C-termini most likely face the lumen. PSI-G constructs in which a His- or Strep-tag is placed at the C-terminus or in the loop region insert with the same topology as wild-type photosys-tem I subunit G (PSI-G).

9p fptmusic 11-04-2013 43 2   Download

Replication protein A (RPA), a heterotrimeric protein composed of 70, 32 and 14-kDa subunits, has been shown to be essential for DNA replication, repair, recombination, and transcription. Previously, we found that, in two seed plants, rice and Arabidopsis, there are two different types of RPA70-kDa subunit. Substantial biochemical and genetic characterization of these two subunits, termed OsRPA70aand OsRPA70bor AtRPA70a and AtRPA70b, respectively, is described in this report.

12p fptmusic 11-04-2013 42 3   Download

PsbO protein is an extrinsic subunit of photosystem II (PSII) and has been proposed to play a central role in stabilization of the catalytic manganese cluster. Arabidopsis thalianahas twopsbO genes that express two PsbO proteins; PsbO1 and PsbO2. We reported previously that a mutant plant that lacked PsbO1 (psbo1) showed considerable growth retardation despite the presence of PsbO2 [Murakami, R., Ifuku, K., Takabayashi, A., Shika-nai, T., Endo, T., and Sato, F. (2002)FEBS Lett523,138–142]. In the pre-sent study, we characterized the functional differences between PsbO1 and PsbO2....

0p awards 06-04-2013 30 2   Download

The conduction of protons through human Nox2 has pre-viously been shown to be dependent upon His115. Align-ment of sequences for both animal and plant Nox proteins indicated that histidines 115 and 119 are both highly con-served, while His111 was conserved among animal homo-logues of Nox1–4. To investigate the possible role that these histidine residues might play in the conduction of protons through Nox2, we have introduced both paired and single mutations into these histidine residues.

8p awards 05-04-2013 43 4   Download

During a search for cDNAs encoding plant sterol acyl-transferases, we isolated four full-length cDNAs fromAra-bidopsis thaliana that encode proteins with substantial identity with animal lecithin : cholesterol acyltransferases (LCATs). The expressionof one of these cDNAs,AtLCAT3 (At3g03310), invarious yeast strains resulted in the doubling of the triacylglycerol content.

13p awards 05-04-2013 39 4   Download

Although the type-2 ribosome-inactivatingproteins (SNA-I, SNA-V, SNLRP) from elderberry (Sambucus nigraL.) are all devoid of rRNAN-glycosylase activity towards plant ribosomes, some of them clearly show polynucleotide– adenosine glycosylase activity towards tobaccomosaic virus RNA. This particular substrate specificity was exploited to further unravel the mechanism underlying the in planta antiviral activity of ribosome-inactivating proteins. Trans-genic tobacco (Nicotiana tabacumL.

8p dell39 03-04-2013 39 4   Download

Volkensin, a type 2 ribosome-inactivating protein from the roots of Adenia volkensiiHarms (kilyambiti plant) was characterized both at the protein and nucleotide level by direct amino acid sequencing and cloning of the gene enco-ding the protein. Gene sequence analysis revealed that vol-kensin is encoded by a 1569-bp ORF (523 amino acid residues) without introns, withan internal linker sequence of 45 bp.

10p dell39 03-04-2013 68 3   Download

A novel, type 1 ribosome-inactivating protein designated charybdin was isolated from bulbs of Charybdis maritimaagg. The protein, consisting of a single polypeptide chain with a molecular mass of 29 kDa, inhibited trans-lation in rabbit reticulocytes with an IC50of 27.2 nm. Plant genomic DNA extracted from the bulb was amplified by PCR between primers based on the N-terminal and C-terminal sequence of the protein from dissolved crys-tals.

9p dell39 27-03-2013 44 3   Download

Peroxisome proliferator-activated receptora(PPARa) is thought to play an important role in lipid metabolism in the liver. To clarify the extra-hepatic and⁄or unknown function of PPARa, we previously performed a proteome analysis of the intestinal proteins and identified 17b-hydroxyster-

9p dell39 27-03-2013 33 3   Download

A phylogenetic analysis of more than 350 multicopper oxidases (MCOs) from fungi, insects, plants, and bacteria provided the basis for a refined classification of this enzyme family into laccasessensu stricto (basidiomyc-etous and ascomycetous), insect laccases, fungal pigment MCOs, fungal ferroxidases, ascorbate oxidases, plant laccase-like MCOs, and bilirubin oxidases.

19p inspiron33 26-03-2013 53 4   Download

Mutational analysis of functional domains in Mrs2p, the mitochondrial Mg2+ chanThe nuclear geneMRS2in Saccharomyces cerevisiaeencodes an integral protein (Mrs2p) of the inner mitochondrial membrane. It forms an ion channel mediating influx of Mg 2+ into mitochondria. Orthologues of Mrs2p have been shown to exist in other lower eukaryotes, in vertebrates and in plants.nel protein of Saccharomyces cerevisiae ` Julian Weghuber, Frank Dieterich, Elisabeth M. Froschauer, Sona Svidova and Rudolf J. Schweyen Max F.

12p inspiron33 26-03-2013 66 5   Download

The virus protein linked to the genome (VPg) of plant potyviruses is a 25-kDa protein covalently attached to the genomic RNA 5¢ end. It was previously reported that VPg binds specifically to eIF4E, the mRNAcap-binding protein of the eukaryotic translation initiation complex. We per-formed a spectroscopic study of the interactions between lettuce eIF4E and VPg from lettuce mosaic virus (LMV).

11p inspiron33 26-03-2013 67 4   Download

We used a proteomic approach to identify constitutively formed extracellu-lar proteins of Hypocrea atroviridis(Trichoderma atroviride), a known bio-control agent. The fungus was cultivated on glucose and the secretome was examined by two-dimensional gel electrophoresis.

14p inspiron33 25-03-2013 35 4   Download

Antimicrobial peptides are an abundant group of proteinaceous com-pounds widely produced in the plant kingdom. Among them, thec-thionin family, also known as plant defensins, represents one typical family and comprises low molecular mass cysteine-rich proteins, usually cationic and distributed in different plant tissues.

9p inspiron33 25-03-2013 31 4   Download

The signal of the plant hormone cytokinin is perceived by membrane-located sensor histidine kinases and transduced by other members of the plant two-component system. InArabidopsis thaliana, 28 two-component system proteins (phosphotransmitters and response regulators) act down-stream of three receptors, transmitting the signal from the membrane to the nucleus and modulating the cellular response.

14p inspiron33 23-03-2013 42 4   Download

Mollicutes are wall-less bacteria and cause various diseases in humans, ani-mals and plants. They have the smallest genomes with low G + C content and lack many genes of DNA, RNA and protein precursor biosynthesis. Nucleoside diphosphate kinase (NDK), a house-keeping enzyme that plays a critical role in the synthesis of nucleic acids precursors, i.e.

8p galaxyss3 19-03-2013 37 4   Download

Mitochondria, which are the cellular energy plants, also act as the integra-tion center of cellular signaling pathways. Apoptosis is a well-known pathway in which mitochondria are involved. Protein kinase Cehas been classified as a novel type of protein kinase C and is involved in many cellular events regulating mitochondrial function.

9p galaxyss3 07-03-2013 29 3   Download