Protein chip
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Luận án "Nghiên cứu phát triển hệ thống phát hiện protein dựa trên hệ thống vi lưu thao tác tập trung protein tích hợp vi cảm biến miễn dịch kiểu tụ phẳng" tập trung nghiên cứu phát triển một hệ thống chip vi kênh tích hợp các chức năng tập trung làm giàu protein, chọn lọc protein đặc hiệu nhằm hướng tới xây dựng và phát triển một thiết bị xét nghiệm nhỏ gọn cho ứng dụng chăm sóc và theo dõi sức khỏe tại chỗ.
131p khanhchi0912 12-04-2024 4 1 Download
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The Wiskott–Aldrich syndrome protein (WASp) family of actin-nucleating factors are present in the cytoplasm and in the nucleus. The role of nuclear WASp for T cell development remains incompletely defined. Methods: We performed WASp chromatin immunoprecipitation and deep sequencing (ChIP-seq) in thymocytes and spleen CD4+ T cells.
18p vioraclene 31-03-2024 7 1 Download
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PPV is one of the most important pathogens causing porcine reproductive disorder. It has been shown in clinical cases to be a commonly mixed infection with other important swine diseases which can aggravate the severity of the disease and bring serious economic losses to the pig industry.
8p vigalileogalilei 28-02-2022 13 1 Download
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At present, pig industry in China is faced with the complex situation of mixed infection caused by multiple pathogens. It is urgent to develop some new high-throughput molecular diagnosis assays to simultaneously detect pathogens or antibodies.
9p vigandhi 23-02-2022 11 1 Download
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Chromatin immunoprecipitation-sequencing (ChIP-seq) is a widely used epigenetic approach for investigating genome-wide protein-DNA interactions in cells and tissues. The approach has been relatively well established but several key steps still require further improvement.
10p vilarryellison 29-10-2021 7 0 Download
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Mục tiêu nghiên cứu của đề tài là phát triển công nghệ polyme in phân tử (MIP) chế tạo đầu thu sinh học nhân tạo trên điện cực mực in các bon phủ hạt nano vàng ứng dụng trong cảm biến sinh học phát hiện định lượng phân tử nhỏ. Nghiên cứu chế tạo cảm biến sinh học phổ tổng trở điện hóa (EIS) sử dụng đầu thu sinh học nhân tạo MIP phát hiện protein (sarcosine và 17β-estradiol), kháng nguyên Enrofloxacin và kháng sinh trong nước hồ nuôi thủy sản và trong dược phẩm.
188p bobietbay 08-10-2021 42 8 Download
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Luận án trình bày các nội dung chính sau: Giới thiệu tổng quan về xét nghiệm protein và các phương pháp xét nghiệm protein; Mô tả nghiên cứu phát triển một chip protein với ba chức năng tập trung, chọn lọc và phát hiện đã được nghiên cứu; Kết quả đạt được của việc phát triển chip tập trung, chọn lọc và phát hiện protein với cấu trúc chip được tích hợp cảm biến điện cực tròn.
30p viyeri2711 14-09-2021 32 3 Download
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For example, gene expression is accurately measured by RNA sequencing (RNA-Seq) libraries, protein-DNA interactions are captured by chromatin immunoprecipitation sequencing (ChIP-Seq), protein-RNA interactions by crosslinking immunoprecipitation sequencing (CLIP-Seq) or RNA immunoprecipitation (RIP-Seq) sequencing, DNA accessibility by assay for transposase-accessible chromatin (ATAC-Seq), DNase or MNase sequencing libraries.
12p viseulgi2711 31-08-2021 10 1 Download
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Chromatin-immunoprecipitation followed by sequencing (ChIP-seq) is the method of choice for mapping genome-wide binding of chromatin-associated factors. However, broadly applicable methods for between-sample comparisons are lacking.
10p vigiselle2711 30-08-2021 7 1 Download
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The study aimed at the utilization of locally available feed resources for increasing growth performance and reducing enteric methane emissions from goats in Lao PDR.
34p nguathienthan12 23-05-2021 26 2 Download
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Chromatin immunoprecipitation coupled with hybridization to a tiling array (ChIP-chip) is a cost-effective and routinely used method to identify protein-DNA interactions or chromatin/histone modifications. The robust identification of ChIP-enriched regions is frequently complicated by noisy measurements.
5p viwyoming2711 16-12-2020 14 1 Download
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Chip-seq experiments are becoming a standard approach for genome-wide profiling protein-DNA interactions, such as detecting transcription factor binding sites, histone modification marks and RNA Polymerase II occupancy.
15p vikentucky2711 24-11-2020 12 1 Download
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Transcription factors (TFs) are proteins that bind to DNA and regulate gene expression. To understand details of gene regulation, characterizing TF binding sites in different cell types, diseases and among individuals is essential. However, sometimes TF binding can only be measured from biological samples that contain multiple cell or tissue types.
12p vioklahoma2711 19-11-2020 10 2 Download
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Transcription factor binding, histone modification, and chromatin accessibility studies are important approaches to understanding the biology of gene regulation. ChIP-seq and DNase-seq have become the standard techniques for studying protein-DNA interactions and chromatin accessibility respectively, and comprehensive quality control (QC) and analysis tools are critical to extracting the most value from these assay types.
13p vioklahoma2711 19-11-2020 13 0 Download
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Functional genomic and epigenomic research relies fundamentally on sequencing based methods like ChIP-seq for the detection of DNA-protein interactions. These techniques return large, high dimensional data sets with visually complex structures, such as multi-modal peaks extended over large genomic regions.
11p vioklahoma2711 19-11-2020 11 1 Download
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ChIP-seq experiments are widely used to detect and study DNA-protein interactions, such as transcription factor binding and chromatin modifications. However, downstream analysis of ChIP-seq data is currently restricted to the evaluation of signal intensity and the detection of enriched regions (peaks) in the genome.
18p vioklahoma2711 19-11-2020 5 0 Download
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Chromatin immunoprecipitation followed by DNA sequencing (ChIP-seq) and associated methods are widely used to define the genome wide distribution of chromatin associated proteins, post-translational epigenetic marks, and modifications found on DNA bases.
16p viconnecticut2711 29-10-2020 15 2 Download
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Chromatin immunoprecipitation coupled to next generation sequencing (ChIP-Seq) is a widely-used molecular method to investigate the function of chromatin-related proteins by identifying their associated DNA sequences on a genomic scale.
17p vicolorado2711 23-10-2020 7 0 Download
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ChIP (Chromatin immunoprecipitation)-exo has emerged as an important and versatile improvement over conventional ChIP-seq as it reduces the level of noise, maps the transcription factor (TF) binding location in a very precise manner, upto single base-pair resolution, and enables binding mode prediction.
14p vicolorado2711 22-10-2020 8 0 Download
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Fluorescence-enhancing microarray on plasmonic gold film is an attractive alternative to traditional enzyme-linked immunosorbent assay (ELISA) for cytokine detection because of the increased sensitivity.
6p viuchiha2711 21-04-2020 7 0 Download