Resonant energy transfer

Dựa trên vị trí cắt đặc hiệu của protease HIV-1, chúng tôi đã thiết kế cơ chất peptide huỳnh quang của protease HIV-1 (ký hiệu peptide HF) theo nguyên tắc FRET (Fluorescence resonance energy transfer).

7p vitheseus2711 28-10-2019 12 0   Download

Trong bài viết này, tác giả xem xét hiện tượng truyền năng lượng cộng hưởng giữa hai phân tử trong sự hiện diện của khối trụ điện môi. Kết quả số cho thấy tốc độ truyền năng lượng có thể tăng hoặc giảm nhiều bậc do ảnh hưởng của khối trụ. Hấp thụ vật chất có xu hướng làm giảm độ truyền năng lượng cộng hưởng, đặc biệt ở các khoảng cách xa.

8p thiendiadaodien_3 27-12-2018 46 3   Download

Trong bài viết này, tác giả xem xét hiện tượng truyền năng lượng cộng hưởng giữa hai phân tử trong sự hiện diện của khối trụ điện môi. Kết quả số cho thấy tốc độ truyền năng lượng có thể tăng hoặc giảm nhiều bậc do ảnh hưởng của khối trụ. Hấp thụ vật chất có xu hướng làm giảm độ truyền năng lượng cộng hưởng, đặc biệt ở các khoảng cách xa.

8p allbymyself_07 02-02-2016 37 5   Download

The binding of Thermomyces lanuginosa lipase and its mutants [TLL(S146A), TLL(W89L), TLL(W117F, W221H, W260H)] to the mixed micelles of cis-parinaric acid/ sodium taurodeoxycholate at pH 5.0 led to the quenching of the intrinsic tryptophan fluorescence emission (300–380 nm) and to a simultaneous increase in the cis-parinaric acid fluorescence emission (380–500 nm). These findings were used to characterize the Thermomyces lanuginosa lipase/cisparinaric acid interactions occurring in the presence of sodium taurodeoxycholate....

9p system191 01-06-2013 49 5   Download

The ¯uorescence resonance energy transfer parameter, f, is de®ned as the eciency of the energy transfer normalized by the quantum yield of the donor in the presence of acceptor. It is possible to characterize the ¯exibility of the protein matrix between the appropriate ¯uorescent probes by monitoring the temperature dependence of f. The intermonomer ¯exibility of the Ca-actin and Mg-actin ®laments was characterized by using this method at pH values of 6.5 and 7.4.

8p research12 01-06-2013 52 4   Download

Mammalian adenylate cyclases are predicted to possess complex topologies, comprising two cassettes of six trans-membrane-spanningmotifs followedbyacytosolic, catalytic ATP-binding domain. Recent studies have begun to provide insights on the tertiary assembly of these proteins; crystal-lographic analysis has revealed that the two cytosolic domains dimerize to forma catalytic core, whilemore recent biochemical and cell biological analysis shows that the two transmembrane cassettes also associate to facilitate the functional assembly and trackingof the enzyme. ...

9p research12 23-04-2013 36 2   Download

The calcium-sensing receptor (CaR) belongs to family C of the G-protein coupled receptor superfamily. The receptor is believed to exist as a homodimer due to covalent and non-covalent interactions between the two amino terminal domains (ATDs). It is well established that agonist binding to family C receptors takes place at the ATD and that this causes theATDdimer to twist.However, very little isknown about the translationof theATDdimer twist intoG-protein coupling to the 7 transmembrane moieties (7TMs) of these receptor dimers....

12p tumor12 22-04-2013 40 2   Download

Human D2Long(D2L)andD2Short(D2S) dopamine receptor isoforms were modified at their N-terminus by the addition of a human immunodeficiency virus (HIV) or a FLAG epitope tag. The receptors were then expressed inSpodop-tera frugiperda 9(Sf9)cells using the baculovirus system, and their oligomerization was investigated by means of co-immunoprecipitation and time-resolved fluorescence resonance energy transfer (FRET).

11p tumor12 20-04-2013 39 2   Download

A new baculovirus-based fluorescence resonance energy transfer (Bv-FRET) assay for measuringmultimerization of cell surface molecules in living cells is described. It has been demonstrated that gonadotropin-releasing hormone recep-tor (GnRH-R) was capable of forming oligomeric com-plexes in the plasma membrane under normal physiological conditions. The mouse gonadotropin-releasing hormone receptor GnRH-R was used to evaluate the efficiency and potential applications of this assay.

9p fptmusic 12-04-2013 58 4   Download

A wide range of approaches has been applied to examine the quaternary structure of G protein-coupled receptors, the basis of such protein–protein interactions and how such interactions might modulate the pharmacology and function of these receptors. These include coimmunoprecipitation, var-ious adaptations of resonance energy transfer techniques, functional com-plementation studies and the analysis of ligand-binding data.

12p fptmusic 11-04-2013 49 3   Download

Rad51 and disrupted meiotic cDNA1 (Dmc1) are the two eukaryotic DNA recombinases that participate in homology search and strand exchange reactions during homologous recombination mediated DNA repair. Rad51 expresses in both mitotic and meiotic tissues whereas Dmc1 is confined to meiosis.

10p inspiron33 26-03-2013 54 5   Download

The tumor suppressor von Hippel–Lindau (VHL) gene product forms a com-plex with elongin B and elongin C, and acts as a recognition subunit of a ubiquitin E3 ligase. Interactions between components in the complex were investigated in living cells by fluorescence resonance energy transfer (FRET)–fluorescence lifetime imaging microscopy (FLIM).

9p media19 04-03-2013 42 2   Download

The production and further metabolism of inositol 1,4,5-trisphosphate [Ins(1,4,5)P3] require several calcium-dependent enzymes, but little is known about subsequent calcium-dependent changes in cellular Ins(1,4,5)P3.

11p media19 04-03-2013 41 3   Download

Loose interaction between the glycolytic enzymes glyceraldehyde-3-phos-phate dehydrogenase (GAPDH) and phosphoglycerate kinase (PGK) was visualized in living CHO-K1 cells by fluorescence resonance energy transfer (FRET), using time-domain fluorescence lifetime imaging microscopy.

9p mobifone23 18-01-2013 44 3   Download

The present study aimed to investigate the role played by the leaflets of the plasma membrane in the uptake of drugs into cells and in their extrusion by P-glycoprotein and multidrug resistance-associated protein 1. Drug accumulation was monitored by fluorescence resonance energy transfer from trimethylammonium-diphenyl-hexatriene (TMA-DPH) located at the outer leaflet to a rhodamine analog.

11p mobifone23 18-01-2013 40 5   Download