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Sanger method
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This case-control study aimed to determine the association between PD-1.9 polymorphism and the risk of HCC in the cohort of chronic HBV-infected patients. Subject and method: Genotyping of PD-1.9 polymorphism was performed by direct sanger sequencing in 499 HBV-infected patients.
7p
vishekhar
01-11-2023
0
0
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To develop a method using polymerase chain reaction with confronting two-pair primers (PCR-CTPP) to genotype MTHFR rs1801133 polymorphism in Vietnamese people. Methods: Fifteen DNA samples from a group of Vietnamese people were used to evaluate this assay. The Sanger sequencing method was used to identify the standard genotypes of the rs1801133 polymorphism.
7p
viwhitewolf
11-07-2023
1
1
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Single-strand conformational polymorphism (SSCP) is still a frequently used genotyping method across different fields for the detection of single nucleotide polymorphisms (SNPs) due to its simplicity, requirement for basic equipment accessible in most laboratories and low cost.
12p
vinarcissa
21-03-2023
3
1
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For this purpose, primary hBM-MSCs with wild type (WT, C/C), heterozygote (HTZ, C/T) and homozygote (HMZ, T/T) for the MTHFR gene were obtained with ethical committee permission and donor informed. Mutations were detected using RFLP and Sanger sequencing methods from genomic DNA isolated from cells, colonization properties were investigated by CFU-F test and proliferative differences were investigated by MTT test.
13p
lyhuyenthu
31-01-2023
7
2
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Total DNA of the anabas specimens (A1, A2, A3, A4) in Vietnam were extracted using PHUSA-IHHNV kit and according to the process of Phu Sa Biochemical Company. The mitochondrial cytochrome oxidase subunit I (COI) gene were amplified using PCR reaction. The obtained PCR products were checked using 2 % agarose gel electrophoresis and were sequenced with both the forward and reverse primers using an automated sequencer ABI 3730XL of Applied Biosystems by Sangers method. The genetic distances (Dij ) between the anabas species in Vietnam (A1, A2, A3, A4) are very small from 0.0046 to 0.
9p
trinhthamhodang9
10-12-2020
15
2
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Culture-independent phylogenetic analysis of 16S ribosomal RNA (rRNA) gene sequences has emerged as an incisive method of profiling bacteria present in a specimen. Currently, multiple techniques are available to enumerate the abundance of bacterial taxa in specimens, including the Sanger sequencing, the ‘next generation’ pyrosequencing, microarrays, quantitative PCR, and the rapidly emerging, third generation sequencing, and fourth generation sequencing methods.
11p
viwyoming2711
16-12-2020
14
0
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Traditional Sanger sequencing has been used as a gold standard method for genetic testing in clinic to perform single gene test, which has been a cumbersome and expensive method to test several genes in heterogeneous disease such as cancer.
9p
vioklahoma2711
19-11-2020
7
1
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Amplicon re-sequencing based on the automated Sanger method remains popular for detection of single nucleotide polymorphisms (SNPs) and insertion-deletion polymorphisms (InDels) for a spectrum of genetics applications.
8p
vioklahoma2711
19-11-2020
6
1
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Today, sequencing is frequently carried out by Massive Parallel Sequencing (MPS) that cuts drastically sequencing time and expenses. Nevertheless, Sanger sequencing remains the main validation method to confirm the presence of variants. The analysis of MPS data involves the development of several bioinformatic tools, academic or commercial.
11p
vioklahoma2711
19-11-2020
10
2
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Targeted Next Generation Sequencing (NGS) assays are cost-efficient and reliable alternatives to Sanger sequencing. For sequencing of very large set of genes, the target enrichment approach is suitable. However, for smaller genomic regions, the target amplification method is more efficient than both the target enrichment method and Sanger sequencing.
9p
viflorida2711
30-10-2020
15
1
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The detection of known human papillomaviruses (PVs) from targeted wet-lab approaches has traditionally used PCR-based methods coupled with Sanger sequencing. With the introduction of next-generation sequencing (NGS), these approaches can be revisited to integrate the sequencing power of NGS.
18p
vicolorado2711
22-10-2020
7
0
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Processing and analysis of DNA sequences obtained from nextgeneration sequencing (NGS) face some difficulties in terms of the correct prediction of DNA sequencing outcomes without the implementation of bioinformatics approaches.
13p
vicolorado2711
22-10-2020
13
0
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High resolution melting curve analysis is a cost-effective rapid screening method for detection of somatic gene mutation. The performance characteristics of this technique has been explored previously, however, analytical parameters such as limit of detection of mutant allele fraction and total concentration of DNA, have not been addressed.
12p
virose2711
25-09-2020
32
0
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Since patients diagnosed with BRAF V600E and V600K mutated advanced melanoma show response to treatment with MAP kinase inhibitors, several sensitive methods have been developed to determine the V600 allele status of melanoma patients.
9p
vilisa271
22-09-2020
13
0
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Germline DNA mutations that increase the susceptibility of a patient to certain cancers have been identified in various genes, and patients can be screened for mutations in these genes to assess their level of risk for developing cancer. Traditional methods using Sanger sequencing focus on small groups of genes and therefore are unable to screen for numerous genes from several patients simultaneously.
11p
vialabama2711
21-09-2020
5
0
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To evaluate the role of FCN2 polymorphism and ficolin-2 protein concentration in pathophysiological disorders in dengue fever; 278 patients with Dengue patients were collected in the 2017 outbreak at Military Hospital 103, diagnosed and grouped according to the “Guidelines for diagnosis and treatment for Dengue of Vietnam Ministry of Health” in 2011, the polymorphisms in the promoter region and exon 8 were determined by Sanger sequencing method. Serum ficolin-2 protein concentration was determined by ELISA method.
10p
kequaidan7
04-09-2020
23
2
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Maple syrup urine disease (MSUD) is an autosomal recessive inherited metabolic disorder caused by malfunction of the branched-chain α-ketoacid dehydrogenase complex (BCKDH). This enzyme complex participates in the catalyzing metabolisms of the branched-chain αketoacids, the second step of the degradation of branched-chain amino acids. Impaired activities of the BCKAD complex lead to an increase of the levels of branched- chain amino acid such as leucine, valine, and isoleucine in the blood.
7p
xilank_of
22-07-2020
32
2
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Lung cancer is one of the quickest and most fatal diseases and is divided into two types: small cell lung cancer (SCLC) and non-small cell lung cancer (NSCLC). Approximately 50% of NSCLC patients harbour mutations in the epidermal growth factor receptor (EGFR) gene, spanning from exon 18 to exon 21, which are responsible for the tyrosine kinase domain of the EGFR protein. Currently, gefitinib and erlotinib are two drugs inhibiting EGFR which are used in the treatment of lung cancer. The indication for use of these drugs depends on the mutation status of EGFR.
8p
caygaocaolon1
13-11-2019
34
1
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Waxy genes of the original variety and its mutant type were sequenced by Sanger method and compared through Nucleotide Basic Local Alignment Search Tool (BLASTN) to clarify differences. BLASTN result showed four nucleotide mutations in coding regions and 59 nucleotide mutations in noncoding regions.
17p
vineptune2711
04-11-2019
14
2
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2. Phương pháp enzyme Sanger Đây là phương pháp tạo đầu tận cùng của chuỗi (chain terminator method). Nguyên lý của phản ứng này là sử dụng dideoxynucleotide không có nhóm OH ở vị trí 3’ trong phản ứng tổng hợp DNA. Do đó, khi DNA polymerase gắn chúng vào sợi DNA thì quá trình tổng hợp bị ngừng lại. Vì vậy, phương pháp này còn gọi là phương pháp dideoxy (dideoxy method). Đầu tiên sợi đôi DNA (sợi khuôn cần đọc trình tự) bị biến tính tách thành hai sợi đơn và được lai với primer. ...
18p
iiduongii9
09-05-2011
95
20
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