Sequencing reaction

Heterogeneous nuclear ribonucleoprotein (HNRNP) A1 and A2 are the most abundant HNRNPs with nearly identical functions, and play important roles in regulating gene expression at multiple levels (i.e. transcription, posttranscription, and translation). However, the expression and regulation mechanism of HNRNPA1 and A2 themselves remain unclear. In this study, the amino acid sequences of HNRNPA1 and HNRNPA2 were compared and found to have 78% and 86% homology in key functional domains.

10p thiencuuchu 27-11-2021 10 1   Download

Hệ kháng nguyên bạch cầu người (HLA, human lymphocyte antigen) nằm trên nhiễm sắc thể thứ 6, đây là một hệ kháng nguyên rất phức tạp và đa dạng. Hệ HLA không những là tiêu chí di truyền của mỗi người, mà đồng thời còn liên hệ chặt chẽ tới chức năng điều hòa miễn dịch và các bệnh tật khác. Mời các bạn cùng tham khảo nghiên cứu.

9p thiendiadaodien_5 08-01-2019 58 2   Download

Hiện nay có nhiều phương pháp để xác định HLA, nhưng phương pháp dựa trên sinh học phân tử là hiện đại, chính xác. Và nghiên cứu tiến hành từ 9/2010 Trung tâm truyền máu Bệnh viện Chợ Rẫy triển khai kỹ thuật HLA (Human Leucocyte Antigen) bằng phương pháp PCR-SSO (Polymerase Chain Reaction – Sequence-specific Oligonucleotide Probes).

4p hanh_tv11 15-01-2019 52 2   Download

Đề tài này được tiến hành nhằm xác định kháng nguyên HLA-A, -B, -C, -DRB1, -DRB3/4/5 và –DQB1 ở người cho và người nhận tế bào gốc tại Bệnh Viện Truyền Máu Huyết Học Thành Phố Hồ Chí Minh, với phương pháp định type HLA ở 203 đối tượng nhờ kỹ thuật polymerase chain reaction-sequence specific primers (PCR-SSP).

5p hanh_tv11 15-01-2019 97 3   Download

Hệ kháng nguyên bạch cầu người (HLA, human lymphocyte antigen) nằm trên nhiễm sắc thể thứ 6, đây là một hệ kháng nguyên rất phức tạp, và đa dạng. Hệ HLA không những là tiêu chí di truyền của mỗi người mà đồng thời còn liên hệ chặt chẽ với chức năng điều hoà miễn dịch và các bệnh tật khác. Những người không cùng một loại hình phân bố các allele của HLA thì tính cảm thụ hay sức đề kháng với một số bệnh không giống nhau. Nhiều tác giả nước ngoài đã nghiên cứu sự khác biệt về phân bố allele HLA - DQA1 trong quần thể người đã phát...

5p sunshine_3 28-06-2013 102 9   Download

Ngày nay, do nhu cầu đời sống của con ngƣời đƣợc nâng cao nên vấn đề thƣ giãn tinh thần ngày càng đƣợc coi trọng. Hiện nay vấn đề chơi cây cảnh trên địa bàn Tp. Hồ Chí Minh (Tp: thành phố) ngày càng phát triển trong đó có xƣơng rồng. Xƣơng rồng đang dần đƣợc khẳng định trên địa bàn Tp. Hồ Chí Minh vì chúng tƣơng đối dễ trồng, giá cả phải chăng, ai cũng có thể chơi và chăm sóc. Xƣơng rồng dù sống trên sa mạc khô cằn vẫn nở hoa sặc sở, thể hiện một...

83p canhchuon_1 19-06-2013 72 16   Download

Previous data showing an increase of receptor binding activity of [R16]VIP, a vasoactive intestinal peptide (VIP) structural analogue containing arginine at the position 16 of its amino acid sequence, have pointed out the importance of a positive charge at this site. Here, the functional characterization of three VIP polyaminated adducts (VIPDap, VIPSpd, and VIPSpm), obtained by a transglutaminasecatalysed reaction between the VIP Gln16 residue and 1,3-diaminopropane (Dap), spermidine (Spd), or spermine (Spm), is reported. ...

9p system191 01-06-2013 42 4   Download

A series of trehalose-based oligosaccharides were isolated from the cytoplasmic fraction of Mycobacterium smegmatis and purified by gel-filtration and paper chromatography and TLC. Their structures were determined by HPLC and GLC to determine sugar composition and ratios, MALDI-TOF MS to measure molecular mass, methylation analysis to determine linkages, 1H-NMR to obtain anomeric configurations of glycosidic linkages, and exoglycosidase digestions followed by TLC to determine sequences and anomeric configurations of the monosaccharides....

8p system191 01-06-2013 36 5   Download

In the biosynthesis of the antiarrhythmic alkaloid ajmaline, polyneuridine aldehyde esterase (PNAE) catalyses a central reaction by transforming polyneuridine aldehyde into epivellosimine, which is the immediate precursor for the synthesis of the ajmalane skeleton. The PNAE cDNA was previously heterologously expressed in E. coli. Sequence alignments indicated that PNAE has a 43% identity to a hydroxynitrile lyase from Hevea brasiliensis, which is a member of the a/b hydrolase superfamily. The catalytic triad, which is typical for this family, is conserved.

8p system191 01-06-2013 35 4   Download

The present work reports isolation and characterization of a highly glycosylated protein from bovine milk fat globule membranes, known as PAS III. Partial amino-acid sequencing of the purified protein allowed construction of degenerate oligonucleotide primers, enabling isolation of a full-length cDNA encoding a protein of 330 amino-acid residues. N-terminal amino-acid sequencing of derived peptides and the purified protein confirmed 76% of the sequence and demonstrated presence of a cleavable signal peptide of 23 residues, leaving a mature protein of 307 amino acids.

9p system191 01-06-2013 49 4   Download

Manganese lipoxygenase was isolated to homogeneity from the take-all fungus, Gaeumannomyces graminis. The C-terminal amino acids and several internal peptides were sequenced, and the information was used to obtain a cDNA probe by RT/PCR. Screening of a genomic library of G. graminis yielded a full-length clone of the Mn-Lipoxygenase gene. cDNA analysis showed that the gene spanned 2.6 kb and contained one intron (133 bp). Northern blot analyses indicated two transcripts (2.7 and 3.1 kb). The deduced amino-acid sequence of the Mn-Lipoxygenase precursor (618 amino acids, 67.

8p system191 01-06-2013 36 4   Download

An exo-1,3-b-glucanase has been isolated from cultural filtrate of Trichoderma viride AZ36. The N-terminal sequence of the purified enzyme (m ¼ 61 ^ 1 kDa) showed no significant homology to other known glucanases. The 1,3-b-glucanase displayed high activity against laminarins, curdlan, and 1,3-b-oligoglucosides, but acted slowly on 1,3-1,4-b-oligoglucosides. No significant activity was detected against high molecular mass 1,3-1,4-bglucans. The enzyme carried out hydrolysis with inversion of the anomeric configuration.

9p system191 01-06-2013 44 6   Download

We report the biochemical characterization of calhepatin, a calcium-binding protein of the S100 family, isolated from lungfish (Lepidosiren paradoxa) liver. The primary structure, determined by Edman degradation and MS/MS, shows that the sequence identities with the other members of the family are lower than those between S100 proteins from different species. Calhepatin is composed of 75 residues and has a molecular mass of 8670 Da. It is smaller than calbindin D9k (78 residues), the smallest S100 described so far....

9p research12 01-06-2013 44 4   Download

A systematic search for Escherichia coli proteins with the zinc-binding activity was performed using the assay of radioactive Zn(II) binding to total E. coli proteins fractionated by two methods of two-dimensional gel electrophoresis. A total of 30–40 radioactive spots were identified, of which 14 have been assigned from N-terminal sequencing.

11p research12 01-06-2013 35 3   Download

Two genes (CM-AAT1 and CM-AAT2) with strong sequence homology (87% identity at the protein level) putatively involved in the formation of aroma volatile esters have been isolated from Charentais melon fruit. They belong to a large and highly divergent family of multifunctional plant acyl-transferases and show at most 21% identity to the only other fruit acyl-transferase characterized so far in strawberry. RT-PCR studies indicated that both genes were specifically expressed in fruit at increasing rates in the early and mid phases of ripening.

8p research12 01-06-2013 49 4   Download

A technique has been developed to search a proteome database for new members of a functional class of membrane protein. It takes advantage of the highly conserved secondary structure of functionally related membrane proteins. Such proteins typically have the same number of transmembrane domains located at similar relative positions in their polypeptide sequence. This gives rise to a characteristic pattern of peaks in their hydropathy profiles.

7p research12 01-06-2013 32 4   Download

A eukaryotic-type protein serine/threonine kinase, PknA, was cloned from Mycobacterium tuberculosis strain H37Ra. Sequencing of the clone indicated 100% identity with the published pknA sequence of M. tuberculosis strain H37Rv. PknA fused to maltose-binding protein was expressed in Escherichia coli; it exhibited a molecular mass of % 97 kDa. The fusion protein was purified from the soluble fraction by affinity chromatography using amylose resin.

8p research12 01-06-2013 41 3   Download

To search for novel regulatory regions, we examined the features of chromatin structure in the rat B29/Ig-b gene and its flanking regions by determining DNase I hypersensitive sites (DHS) in plasmacytoma-derived Y3 cells. Six Y3 cellspecific DHS were detected at )8.6, promoter, +0.7, +4.4, +6.0, and +8.7 kb. The DHS at +4.4, +6.0, and +8.7 kb were present in the intergenic region between B29/Ig-b and growth hormone (GH) genes and were mapped inside conserved sequences in rat and humans.

10p research12 01-06-2013 29 4   Download

A gene having high sequence homology (45–49%) with the glycerol-1-phosphate dehydrogenase gene from Methanobacterium thermoautotrophicum was cloned from the aerobic hyperthermophilic archaeon Aeropyrum pernix K1 (JCM 9820). This gene expressed in Escherichia coli with the pET vector system consists of 1113 nucleotides with an ATG initiation codon and a TAG termination codon. The molecular mass of the purified enzyme was estimated to be 38 kDa by SDS/PAGE and 72.4 kDa by gel column chromatography, indicating presence as a dimer....

8p research12 01-06-2013 27 4   Download

Mitogen activated protein kinase kinase (MKK) 3 and 6 are the main p38 mitogen-activated protein kinase activators in mammals. In the present study, three Atlantic salmon MKK6 orthologs were identified. The deduced amino acid sequences of the salmon MKK6 proteins were highly similar to mammalian MKK6 sequences, and they were ubiquitously expressed. All three were shown to be upstream activators of salmon p38.

16p research12 29-04-2013 27 3   Download